Standardization of an in Vitro Regeneration Protocol in Gerbera (Gerbera jamesonii Bolus Ex. Hooker F.)

An experiment was undertaken to develop an improved in vitro regeneration protocol in gerbera. Murashige and Skoog (MS) medium was supplemented with various growth regulators at different concentrations for callus induction and organogenesis. Newly emerging leaves of Gerbera cv. Rosalin were used as...

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Autores principales:	Koushik Dutta, Subhendu S Gantait
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2016
Materias:	gerbera gerbera jamesonii leaf explants callus in vitro regeneration. Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/a8c3740583154bec98e8a358a4f88bd9
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spelling	oai:doaj.org-article:a8c3740583154bec98e8a358a4f88bd92021-12-02T12:39:58ZStandardization of an in Vitro Regeneration Protocol in Gerbera (Gerbera jamesonii Bolus Ex. Hooker F.)0973-354X2582-4899https://doaj.org/article/a8c3740583154bec98e8a358a4f88bd92016-12-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/84https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899An experiment was undertaken to develop an improved in vitro regeneration protocol in gerbera. Murashige and Skoog (MS) medium was supplemented with various growth regulators at different concentrations for callus induction and organogenesis. Newly emerging leaves of Gerbera cv. Rosalin were used as explants. Experimental results showed that maximum rate (74.07%) of formation of callus with good growth was recorded on MS medium supplemented with 2.0mgL-1 2,4-D + BAP 0.5mgL-1. Best shoot regeneration (57.8 %) with maximum shoot number (12.0) was achieved on with BAP 2.0mgL-1 + NAA 0.5mgL-1 fortified MS medium. Maximum (66.7 %) and earliest (12.3 days) root formation in shoots was recorded on IBA 3.0mgL-1is 1/2MS media. Survival rate of regenerated plantlets was maximum (73.33 %) in the potting mixture containing garden soil, sand and vermicompost (1:1:1).Koushik DuttaSubhendu S GantaitSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlegerberagerbera jamesoniileaf explantscallusin vitro regeneration.Plant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 11, Iss 2, Pp 143-150 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	gerbera gerbera jamesonii leaf explants callus in vitro regeneration. Plant culture SB1-1110
spellingShingle	gerbera gerbera jamesonii leaf explants callus in vitro regeneration. Plant culture SB1-1110 Koushik Dutta Subhendu S Gantait Standardization of an in Vitro Regeneration Protocol in Gerbera (Gerbera jamesonii Bolus Ex. Hooker F.)
description	An experiment was undertaken to develop an improved in vitro regeneration protocol in gerbera. Murashige and Skoog (MS) medium was supplemented with various growth regulators at different concentrations for callus induction and organogenesis. Newly emerging leaves of Gerbera cv. Rosalin were used as explants. Experimental results showed that maximum rate (74.07%) of formation of callus with good growth was recorded on MS medium supplemented with 2.0mgL-1 2,4-D + BAP 0.5mgL-1. Best shoot regeneration (57.8 %) with maximum shoot number (12.0) was achieved on with BAP 2.0mgL-1 + NAA 0.5mgL-1 fortified MS medium. Maximum (66.7 %) and earliest (12.3 days) root formation in shoots was recorded on IBA 3.0mgL-1is 1/2MS media. Survival rate of regenerated plantlets was maximum (73.33 %) in the potting mixture containing garden soil, sand and vermicompost (1:1:1).
format	article
author	Koushik Dutta Subhendu S Gantait
author_facet	Koushik Dutta Subhendu S Gantait
author_sort	Koushik Dutta
title	Standardization of an in Vitro Regeneration Protocol in Gerbera (Gerbera jamesonii Bolus Ex. Hooker F.)
title_short	Standardization of an in Vitro Regeneration Protocol in Gerbera (Gerbera jamesonii Bolus Ex. Hooker F.)
title_full	Standardization of an in Vitro Regeneration Protocol in Gerbera (Gerbera jamesonii Bolus Ex. Hooker F.)
title_fullStr	Standardization of an in Vitro Regeneration Protocol in Gerbera (Gerbera jamesonii Bolus Ex. Hooker F.)
title_full_unstemmed	Standardization of an in Vitro Regeneration Protocol in Gerbera (Gerbera jamesonii Bolus Ex. Hooker F.)
title_sort	standardization of an in vitro regeneration protocol in gerbera (gerbera jamesonii bolus ex. hooker f.)
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2016
url	https://doaj.org/article/a8c3740583154bec98e8a358a4f88bd9
work_keys_str_mv	AT koushikdutta standardizationofaninvitroregenerationprotocolingerberagerberajamesoniibolusexhookerf AT subhendusgantait standardizationofaninvitroregenerationprotocolingerberagerberajamesoniibolusexhookerf
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Standardization of an in Vitro Regeneration Protocol in Gerbera (Gerbera jamesonii Bolus Ex. Hooker F.)

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