DEVELOPMENT OF FUSION RECOMBINANT PROTEINS BASED ON VP6 AND VP8 OF HUMAN ROTAVIRUS A
Rotaviruses are the most frequent cause of children enteritis. This infection often leads to severe dehydration of organism. Many infectious diseases are accompanied by fluid loss being the most common cause of death. Nowadays an early vaccination is considered to be the most effective way for preve...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | article |
| Lenguaje: | RU |
| Publicado: |
Sankt-Peterburg : NIIÈM imeni Pastera
2014
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/a8c80c2db3fb4d84a137bd749529a115 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: | Rotaviruses are the most frequent cause of children enteritis. This infection often leads to severe dehydration of organism. Many infectious diseases are accompanied by fluid loss being the most common cause of death. Nowadays an early vaccination is considered to be the most effective way for prevention of rotavirus infection. However attenuated alive vaccines are used for this purpose which can result in different complications. The candidate vaccine presented in this study was designed on the basis of recombinant fusion proteins — the crucial active agents for development of immune response against rotaviruses. As part of the study the following steps were taken: the design of fusion proteins VP6VP8 and FliCVP6VP8; protocol adjustment for induction of genes encoding these proteins expression, which yields in high level of the protein with minor expenditures; production and purification of recombinant proteins VP6VP8 and FliCVP6VP8 with metal affinity chromatography. |
|---|