DEVELOPMENT OF FUSION RECOMBINANT PROTEINS BASED ON VP6 AND VP8 OF HUMAN ROTAVIRUS A

Rotaviruses are the most frequent cause of children enteritis. This infection often leads to severe dehydration of organism. Many infectious diseases are accompanied by fluid loss being the most common cause of death. Nowadays an early vaccination is considered to be the most effective way for preve...

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Autores principales:	I. V. Dukhovlinov, E. G. Bogomolova, E. A. Fedorova, A. S. Simbirtsev
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2014
Materias:	rotavirus gastroenteritis vaccine recombinant fusion proteins. Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/a8c80c2db3fb4d84a137bd749529a115
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spelling	oai:doaj.org-article:a8c80c2db3fb4d84a137bd749529a1152021-11-22T07:09:47ZDEVELOPMENT OF FUSION RECOMBINANT PROTEINS BASED ON VP6 AND VP8 OF HUMAN ROTAVIRUS A2220-76192313-739810.15789/2220-7619-2014-3-229-234https://doaj.org/article/a8c80c2db3fb4d84a137bd749529a1152014-08-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/181https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Rotaviruses are the most frequent cause of children enteritis. This infection often leads to severe dehydration of organism. Many infectious diseases are accompanied by fluid loss being the most common cause of death. Nowadays an early vaccination is considered to be the most effective way for prevention of rotavirus infection. However attenuated alive vaccines are used for this purpose which can result in different complications. The candidate vaccine presented in this study was designed on the basis of recombinant fusion proteins — the crucial active agents for development of immune response against rotaviruses. As part of the study the following steps were taken: the design of fusion proteins VP6VP8 and FliCVP6VP8; protocol adjustment for induction of genes encoding these proteins expression, which yields in high level of the protein with minor expenditures; production and purification of recombinant proteins VP6VP8 and FliCVP6VP8 with metal affinity chromatography.I. V. DukhovlinovE. G. BogomolovaE. A. FedorovaA. S. SimbirtsevSankt-Peterburg : NIIÈM imeni Pasteraarticlerotavirus gastroenteritisvaccinerecombinant fusion proteins.Infectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 4, Iss 3, Pp 229-234 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	rotavirus gastroenteritis vaccine recombinant fusion proteins. Infectious and parasitic diseases RC109-216
spellingShingle	rotavirus gastroenteritis vaccine recombinant fusion proteins. Infectious and parasitic diseases RC109-216 I. V. Dukhovlinov E. G. Bogomolova E. A. Fedorova A. S. Simbirtsev DEVELOPMENT OF FUSION RECOMBINANT PROTEINS BASED ON VP6 AND VP8 OF HUMAN ROTAVIRUS A
description	Rotaviruses are the most frequent cause of children enteritis. This infection often leads to severe dehydration of organism. Many infectious diseases are accompanied by fluid loss being the most common cause of death. Nowadays an early vaccination is considered to be the most effective way for prevention of rotavirus infection. However attenuated alive vaccines are used for this purpose which can result in different complications. The candidate vaccine presented in this study was designed on the basis of recombinant fusion proteins — the crucial active agents for development of immune response against rotaviruses. As part of the study the following steps were taken: the design of fusion proteins VP6VP8 and FliCVP6VP8; protocol adjustment for induction of genes encoding these proteins expression, which yields in high level of the protein with minor expenditures; production and purification of recombinant proteins VP6VP8 and FliCVP6VP8 with metal affinity chromatography.
format	article
author	I. V. Dukhovlinov E. G. Bogomolova E. A. Fedorova A. S. Simbirtsev
author_facet	I. V. Dukhovlinov E. G. Bogomolova E. A. Fedorova A. S. Simbirtsev
author_sort	I. V. Dukhovlinov
title	DEVELOPMENT OF FUSION RECOMBINANT PROTEINS BASED ON VP6 AND VP8 OF HUMAN ROTAVIRUS A
title_short	DEVELOPMENT OF FUSION RECOMBINANT PROTEINS BASED ON VP6 AND VP8 OF HUMAN ROTAVIRUS A
title_full	DEVELOPMENT OF FUSION RECOMBINANT PROTEINS BASED ON VP6 AND VP8 OF HUMAN ROTAVIRUS A
title_fullStr	DEVELOPMENT OF FUSION RECOMBINANT PROTEINS BASED ON VP6 AND VP8 OF HUMAN ROTAVIRUS A
title_full_unstemmed	DEVELOPMENT OF FUSION RECOMBINANT PROTEINS BASED ON VP6 AND VP8 OF HUMAN ROTAVIRUS A
title_sort	development of fusion recombinant proteins based on vp6 and vp8 of human rotavirus a
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2014
url	https://doaj.org/article/a8c80c2db3fb4d84a137bd749529a115
work_keys_str_mv	AT ivdukhovlinov developmentoffusionrecombinantproteinsbasedonvp6andvp8ofhumanrotavirusa AT egbogomolova developmentoffusionrecombinantproteinsbasedonvp6andvp8ofhumanrotavirusa AT eafedorova developmentoffusionrecombinantproteinsbasedonvp6andvp8ofhumanrotavirusa AT assimbirtsev developmentoffusionrecombinantproteinsbasedonvp6andvp8ofhumanrotavirusa
_version_	1718418060488998912

DEVELOPMENT OF FUSION RECOMBINANT PROTEINS BASED ON VP6 AND VP8 OF HUMAN ROTAVIRUS A

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