Role of Episamarcandin in Promoting the Apoptosis of Human Colon Cancer HCT116 Cells through the PI3K-Akt Signaling Pathway

This study identifies the active ingredients of Ferula sinkiangensis and investigates the role and mechanism of episamarcandin in colon cancer cells. The silica gel column chromatography was utilized to separate the chemical components of Ferula sinkiangensis. Sephadex LH-20 and semipreparative HPLC...

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Autores principales:	Haiying Zhang, Jianan Sun, Ruoting Ma, Shengjun Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Other systems of medicine RZ201-999
Acceso en línea:	https://doaj.org/article/a8ce2ac4d37c40f0ae692ef33f7c7dc0
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spelling	oai:doaj.org-article:a8ce2ac4d37c40f0ae692ef33f7c7dc02021-11-15T01:19:47ZRole of Episamarcandin in Promoting the Apoptosis of Human Colon Cancer HCT116 Cells through the PI3K-Akt Signaling Pathway1741-428810.1155/2021/9663738https://doaj.org/article/a8ce2ac4d37c40f0ae692ef33f7c7dc02021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/9663738https://doaj.org/toc/1741-4288This study identifies the active ingredients of Ferula sinkiangensis and investigates the role and mechanism of episamarcandin in colon cancer cells. The silica gel column chromatography was utilized to separate the chemical components of Ferula sinkiangensis. Sephadex LH-20 and semipreparative HPLC were adopted for further separation and purification. The compound episamarcandin showed good anticolon cancer activity among the 13 monomeric compounds obtained. Its effects on the apoptosis, cell cycle, and invasion and migration of colon cancer HCT 116 cells and PI3K-Akt signaling pathway were further investigated. The results showed that, similar to positive control cisplatin, episamarcandin inhibited the proliferation, promoted the apoptosis, arrested cells at G0/G1 phase, and suppressed migration and invasion of HCT 116 cells. A large number of apoptotic HCT 116 cells were observed under a transmission electron microscope. Fluorescence real-time quantitative PCR and western blot analysis showed that episamarcandin increased the expression of PTEN, p53, and Bax and decreased the expression of P-Akt, Akt, mTOR, Bcl-xl, and Bcl-2. Conclusively, episamarcandin may inhibit cell proliferation, migration, and invasion and promote the apoptosis of human colon cancer HCT 116 cells possibly through the PI3K-Akt signaling pathway.Haiying ZhangJianan SunRuoting MaShengjun ZhaoHindawi LimitedarticleOther systems of medicineRZ201-999ENEvidence-Based Complementary and Alternative Medicine, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Other systems of medicine RZ201-999
spellingShingle	Other systems of medicine RZ201-999 Haiying Zhang Jianan Sun Ruoting Ma Shengjun Zhao Role of Episamarcandin in Promoting the Apoptosis of Human Colon Cancer HCT116 Cells through the PI3K-Akt Signaling Pathway
description	This study identifies the active ingredients of Ferula sinkiangensis and investigates the role and mechanism of episamarcandin in colon cancer cells. The silica gel column chromatography was utilized to separate the chemical components of Ferula sinkiangensis. Sephadex LH-20 and semipreparative HPLC were adopted for further separation and purification. The compound episamarcandin showed good anticolon cancer activity among the 13 monomeric compounds obtained. Its effects on the apoptosis, cell cycle, and invasion and migration of colon cancer HCT 116 cells and PI3K-Akt signaling pathway were further investigated. The results showed that, similar to positive control cisplatin, episamarcandin inhibited the proliferation, promoted the apoptosis, arrested cells at G0/G1 phase, and suppressed migration and invasion of HCT 116 cells. A large number of apoptotic HCT 116 cells were observed under a transmission electron microscope. Fluorescence real-time quantitative PCR and western blot analysis showed that episamarcandin increased the expression of PTEN, p53, and Bax and decreased the expression of P-Akt, Akt, mTOR, Bcl-xl, and Bcl-2. Conclusively, episamarcandin may inhibit cell proliferation, migration, and invasion and promote the apoptosis of human colon cancer HCT 116 cells possibly through the PI3K-Akt signaling pathway.
format	article
author	Haiying Zhang Jianan Sun Ruoting Ma Shengjun Zhao
author_facet	Haiying Zhang Jianan Sun Ruoting Ma Shengjun Zhao
author_sort	Haiying Zhang
title	Role of Episamarcandin in Promoting the Apoptosis of Human Colon Cancer HCT116 Cells through the PI3K-Akt Signaling Pathway
title_short	Role of Episamarcandin in Promoting the Apoptosis of Human Colon Cancer HCT116 Cells through the PI3K-Akt Signaling Pathway
title_full	Role of Episamarcandin in Promoting the Apoptosis of Human Colon Cancer HCT116 Cells through the PI3K-Akt Signaling Pathway
title_fullStr	Role of Episamarcandin in Promoting the Apoptosis of Human Colon Cancer HCT116 Cells through the PI3K-Akt Signaling Pathway
title_full_unstemmed	Role of Episamarcandin in Promoting the Apoptosis of Human Colon Cancer HCT116 Cells through the PI3K-Akt Signaling Pathway
title_sort	role of episamarcandin in promoting the apoptosis of human colon cancer hct116 cells through the pi3k-akt signaling pathway
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/a8ce2ac4d37c40f0ae692ef33f7c7dc0
work_keys_str_mv	AT haiyingzhang roleofepisamarcandininpromotingtheapoptosisofhumancoloncancerhct116cellsthroughthepi3kaktsignalingpathway AT jianansun roleofepisamarcandininpromotingtheapoptosisofhumancoloncancerhct116cellsthroughthepi3kaktsignalingpathway AT ruotingma roleofepisamarcandininpromotingtheapoptosisofhumancoloncancerhct116cellsthroughthepi3kaktsignalingpathway AT shengjunzhao roleofepisamarcandininpromotingtheapoptosisofhumancoloncancerhct116cellsthroughthepi3kaktsignalingpathway
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Role of Episamarcandin in Promoting the Apoptosis of Human Colon Cancer HCT116 Cells through the PI3K-Akt Signaling Pathway

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