Par3 cooperates with Sanpodo for the assembly of Notch clusters following asymmetric division of Drosophila sensory organ precursor cells

In multiple cell lineages, Delta-Notch signalling regulates cell fate decisions owing to unidirectional signalling between daughter cells. In Drosophila pupal sensory organ lineage, Notch regulates the intra-lineage pIIa/pIIb fate decision at cytokinesis. Notch and Delta that localise apically and b...

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Autores principales:	Elise Houssin, Mathieu Pinot, Karen Bellec, Roland Le Borgne
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	epithelium Notch signalling polarity Par3 asymmetric cell division Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a8d431190d1a42ecb228438407cd32eb
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spelling	oai:doaj.org-article:a8d431190d1a42ecb228438407cd32eb2021-11-15T06:06:46ZPar3 cooperates with Sanpodo for the assembly of Notch clusters following asymmetric division of Drosophila sensory organ precursor cells10.7554/eLife.666592050-084Xe66659https://doaj.org/article/a8d431190d1a42ecb228438407cd32eb2021-10-01T00:00:00Zhttps://elifesciences.org/articles/66659https://doaj.org/toc/2050-084XIn multiple cell lineages, Delta-Notch signalling regulates cell fate decisions owing to unidirectional signalling between daughter cells. In Drosophila pupal sensory organ lineage, Notch regulates the intra-lineage pIIa/pIIb fate decision at cytokinesis. Notch and Delta that localise apically and basally at the pIIa-pIIb interface are expressed at low levels and their residence time at the plasma membrane is in the order of minutes. How Delta can effectively interact with Notch to trigger signalling from a large plasma membrane area remains poorly understood. Here, we report that the signalling interface possesses a unique apico-basal polarity with Par3/Bazooka localising in the form of nano-clusters at the apical and basal level. Notch is preferentially targeted to the pIIa-pIIb interface, where it co-clusters with Bazooka and its cofactor Sanpodo. Clusters whose assembly relies on Bazooka and Sanpodo activities are also positive for Neuralized, the E3 ligase required for Delta activity. We propose that the nano-clusters act as snap buttons at the new pIIa-pIIb interface to allow efficient intra-lineage signalling.Elise HoussinMathieu PinotKaren BellecRoland Le BorgneeLife Sciences Publications LtdarticleepitheliumNotch signallingpolarityPar3asymmetric cell divisionMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	epithelium Notch signalling polarity Par3 asymmetric cell division Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	epithelium Notch signalling polarity Par3 asymmetric cell division Medicine R Science Q Biology (General) QH301-705.5 Elise Houssin Mathieu Pinot Karen Bellec Roland Le Borgne Par3 cooperates with Sanpodo for the assembly of Notch clusters following asymmetric division of Drosophila sensory organ precursor cells
description	In multiple cell lineages, Delta-Notch signalling regulates cell fate decisions owing to unidirectional signalling between daughter cells. In Drosophila pupal sensory organ lineage, Notch regulates the intra-lineage pIIa/pIIb fate decision at cytokinesis. Notch and Delta that localise apically and basally at the pIIa-pIIb interface are expressed at low levels and their residence time at the plasma membrane is in the order of minutes. How Delta can effectively interact with Notch to trigger signalling from a large plasma membrane area remains poorly understood. Here, we report that the signalling interface possesses a unique apico-basal polarity with Par3/Bazooka localising in the form of nano-clusters at the apical and basal level. Notch is preferentially targeted to the pIIa-pIIb interface, where it co-clusters with Bazooka and its cofactor Sanpodo. Clusters whose assembly relies on Bazooka and Sanpodo activities are also positive for Neuralized, the E3 ligase required for Delta activity. We propose that the nano-clusters act as snap buttons at the new pIIa-pIIb interface to allow efficient intra-lineage signalling.
format	article
author	Elise Houssin Mathieu Pinot Karen Bellec Roland Le Borgne
author_facet	Elise Houssin Mathieu Pinot Karen Bellec Roland Le Borgne
author_sort	Elise Houssin
title	Par3 cooperates with Sanpodo for the assembly of Notch clusters following asymmetric division of Drosophila sensory organ precursor cells
title_short	Par3 cooperates with Sanpodo for the assembly of Notch clusters following asymmetric division of Drosophila sensory organ precursor cells
title_full	Par3 cooperates with Sanpodo for the assembly of Notch clusters following asymmetric division of Drosophila sensory organ precursor cells
title_fullStr	Par3 cooperates with Sanpodo for the assembly of Notch clusters following asymmetric division of Drosophila sensory organ precursor cells
title_full_unstemmed	Par3 cooperates with Sanpodo for the assembly of Notch clusters following asymmetric division of Drosophila sensory organ precursor cells
title_sort	par3 cooperates with sanpodo for the assembly of notch clusters following asymmetric division of drosophila sensory organ precursor cells
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/a8d431190d1a42ecb228438407cd32eb
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Par3 cooperates with Sanpodo for the assembly of Notch clusters following asymmetric division of Drosophila sensory organ precursor cells

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