Methodological deficits in diagnostic research using '-omics' technologies: evaluation of the QUADOMICS tool and quality of recently published studies.

<h4>Background</h4>QUADOMICS is an adaptation of QUADAS (a quality assessment tool for use in systematic reviews of diagnostic accuracy studies), which takes into account the particular challenges presented by '-omics' based technologies. Our primary objective was to evaluate t...

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Autores principales:	Lucy A Parker, Noemí Gómez Saez, Blanca Lumbreras, Miquel Porta, Ildefonso Hernández-Aguado
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a8db65645bcd4d189cc263f327d11594
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spelling	oai:doaj.org-article:a8db65645bcd4d189cc263f327d115942021-12-02T20:20:19ZMethodological deficits in diagnostic research using '-omics' technologies: evaluation of the QUADOMICS tool and quality of recently published studies.1932-620310.1371/journal.pone.0011419https://doaj.org/article/a8db65645bcd4d189cc263f327d115942010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20625481/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>QUADOMICS is an adaptation of QUADAS (a quality assessment tool for use in systematic reviews of diagnostic accuracy studies), which takes into account the particular challenges presented by '-omics' based technologies. Our primary objective was to evaluate the applicability and consistency of QUADOMICS. Subsequently we evaluated and describe the methodological quality of a sample of recently published studies using the tool.<h4>Methodology/principal findings</h4>45'-omics'-based diagnostic studies were identified by systematic search of Pubmed using suitable MeSH terms ("Genomics", "Sensitivity and specificity", "Diagnosis"). Three investigators independently assessed the quality of the articles using QUADOMICS and met to compare observations and generate a consensus. Consistency and applicability was assessed by comparing each reviewer's original rating with the consensus. Methodological quality was described using the consensus rating. Agreement was above 80% for all three reviewers. Four items presented difficulties with application, mostly due to the lack of a clearly defined gold standard. Methodological quality of our sample was poor; studies met roughly half of the applied criteria (mean +/- sd, 54.7+/-18.4%). Few studies were carried out in a population that mirrored the clinical situation in which the test would be used in practice, (6, 13.3%); none described patient recruitment sufficiently; and less than half described clinical and physiological factors that might influence the biomarker profile (20, 44.4%).<h4>Conclusions</h4>The QUADOMICS tool can consistently be applied to diagnostic '-omics' studies presently published in biomedical journals. A substantial proportion of reports in this research field fail to address design issues that are fundamental to make inferences relevant for patient care.Lucy A ParkerNoemí Gómez SaezBlanca LumbrerasMiquel PortaIldefonso Hernández-AguadoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 7, p e11419 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucy A Parker Noemí Gómez Saez Blanca Lumbreras Miquel Porta Ildefonso Hernández-Aguado Methodological deficits in diagnostic research using '-omics' technologies: evaluation of the QUADOMICS tool and quality of recently published studies.
description	<h4>Background</h4>QUADOMICS is an adaptation of QUADAS (a quality assessment tool for use in systematic reviews of diagnostic accuracy studies), which takes into account the particular challenges presented by '-omics' based technologies. Our primary objective was to evaluate the applicability and consistency of QUADOMICS. Subsequently we evaluated and describe the methodological quality of a sample of recently published studies using the tool.<h4>Methodology/principal findings</h4>45'-omics'-based diagnostic studies were identified by systematic search of Pubmed using suitable MeSH terms ("Genomics", "Sensitivity and specificity", "Diagnosis"). Three investigators independently assessed the quality of the articles using QUADOMICS and met to compare observations and generate a consensus. Consistency and applicability was assessed by comparing each reviewer's original rating with the consensus. Methodological quality was described using the consensus rating. Agreement was above 80% for all three reviewers. Four items presented difficulties with application, mostly due to the lack of a clearly defined gold standard. Methodological quality of our sample was poor; studies met roughly half of the applied criteria (mean +/- sd, 54.7+/-18.4%). Few studies were carried out in a population that mirrored the clinical situation in which the test would be used in practice, (6, 13.3%); none described patient recruitment sufficiently; and less than half described clinical and physiological factors that might influence the biomarker profile (20, 44.4%).<h4>Conclusions</h4>The QUADOMICS tool can consistently be applied to diagnostic '-omics' studies presently published in biomedical journals. A substantial proportion of reports in this research field fail to address design issues that are fundamental to make inferences relevant for patient care.
format	article
author	Lucy A Parker Noemí Gómez Saez Blanca Lumbreras Miquel Porta Ildefonso Hernández-Aguado
author_facet	Lucy A Parker Noemí Gómez Saez Blanca Lumbreras Miquel Porta Ildefonso Hernández-Aguado
author_sort	Lucy A Parker
title	Methodological deficits in diagnostic research using '-omics' technologies: evaluation of the QUADOMICS tool and quality of recently published studies.
title_short	Methodological deficits in diagnostic research using '-omics' technologies: evaluation of the QUADOMICS tool and quality of recently published studies.
title_full	Methodological deficits in diagnostic research using '-omics' technologies: evaluation of the QUADOMICS tool and quality of recently published studies.
title_fullStr	Methodological deficits in diagnostic research using '-omics' technologies: evaluation of the QUADOMICS tool and quality of recently published studies.
title_full_unstemmed	Methodological deficits in diagnostic research using '-omics' technologies: evaluation of the QUADOMICS tool and quality of recently published studies.
title_sort	methodological deficits in diagnostic research using '-omics' technologies: evaluation of the quadomics tool and quality of recently published studies.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/a8db65645bcd4d189cc263f327d11594
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Methodological deficits in diagnostic research using '-omics' technologies: evaluation of the QUADOMICS tool and quality of recently published studies.

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