MicroRNA and transcriptome analysis in periocular Sebaceous Gland Carcinoma

Abstract Sebaceous gland carcinoma (SGC) is a rare, but life-threatening condition with a predilection for the periocular region. Eyelid SGC can be broadly categorised into two subtypes, namely either nodular or pagetoid with the latter being more aggressive and requiring radical excision to save li...

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Autores principales:	John C. Bladen, Jun Wang, Ajanthah Sangaralingam, Mariya Moosajee, Caroline Fitchett, Claude Chelala, Michele Beaconsfield, Edel A. O’Toole, Michael P. Philpott, Daniel G. Ezra
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a8dbd7b7e4544abea6e78840cdc42587
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spelling	oai:doaj.org-article:a8dbd7b7e4544abea6e78840cdc425872021-12-02T16:08:03ZMicroRNA and transcriptome analysis in periocular Sebaceous Gland Carcinoma10.1038/s41598-018-25900-z2045-2322https://doaj.org/article/a8dbd7b7e4544abea6e78840cdc425872018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25900-zhttps://doaj.org/toc/2045-2322Abstract Sebaceous gland carcinoma (SGC) is a rare, but life-threatening condition with a predilection for the periocular region. Eyelid SGC can be broadly categorised into two subtypes, namely either nodular or pagetoid with the latter being more aggressive and requiring radical excision to save life. We have identified key altered microRNAs (miRNA) involved in SGC shared by both subtypes, hsa-miR-34a-5p and hsa-miR-16-5p. However, their gene targets BCL2 and MYC were differentially expressed with both overexpressed in pagetoid but unchanged in nodular suggesting different modes of action of these two miRNAs on BCL/MYC expression. Hsa-miR-150p is nodular-specifically overexpressed, and its target ZEB1 was significantly downregulated in nodular SGC suggesting a tumour suppressor role. Invasive pagetoid subtype demonstrated specific overexpression of hsa-miR-205 and downregulation of hsa-miR-199a. Correspondingly, miRNA gene targets, EZH2 (by hsa-miR-205) and CD44 (by hsa-miR-199a), were both overexpressed in pagetoid SGC. CD44 has been identified as a potential cancer stem cell marker in head and neck squamous cell carcinoma and its overexpression in pagetoid cells represents a novel treatment target. Aberrant miRNAs and their gene targets have been identified in both SGC subtypes, paving the way for better molecular understanding of these tumours and identifying new treatment targets.John C. BladenJun WangAjanthah SangaralingamMariya MoosajeeCaroline FitchettClaude ChelalaMichele BeaconsfieldEdel A. O’TooleMichael P. PhilpottDaniel G. EzraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q John C. Bladen Jun Wang Ajanthah Sangaralingam Mariya Moosajee Caroline Fitchett Claude Chelala Michele Beaconsfield Edel A. O’Toole Michael P. Philpott Daniel G. Ezra MicroRNA and transcriptome analysis in periocular Sebaceous Gland Carcinoma
description	Abstract Sebaceous gland carcinoma (SGC) is a rare, but life-threatening condition with a predilection for the periocular region. Eyelid SGC can be broadly categorised into two subtypes, namely either nodular or pagetoid with the latter being more aggressive and requiring radical excision to save life. We have identified key altered microRNAs (miRNA) involved in SGC shared by both subtypes, hsa-miR-34a-5p and hsa-miR-16-5p. However, their gene targets BCL2 and MYC were differentially expressed with both overexpressed in pagetoid but unchanged in nodular suggesting different modes of action of these two miRNAs on BCL/MYC expression. Hsa-miR-150p is nodular-specifically overexpressed, and its target ZEB1 was significantly downregulated in nodular SGC suggesting a tumour suppressor role. Invasive pagetoid subtype demonstrated specific overexpression of hsa-miR-205 and downregulation of hsa-miR-199a. Correspondingly, miRNA gene targets, EZH2 (by hsa-miR-205) and CD44 (by hsa-miR-199a), were both overexpressed in pagetoid SGC. CD44 has been identified as a potential cancer stem cell marker in head and neck squamous cell carcinoma and its overexpression in pagetoid cells represents a novel treatment target. Aberrant miRNAs and their gene targets have been identified in both SGC subtypes, paving the way for better molecular understanding of these tumours and identifying new treatment targets.
format	article
author	John C. Bladen Jun Wang Ajanthah Sangaralingam Mariya Moosajee Caroline Fitchett Claude Chelala Michele Beaconsfield Edel A. O’Toole Michael P. Philpott Daniel G. Ezra
author_facet	John C. Bladen Jun Wang Ajanthah Sangaralingam Mariya Moosajee Caroline Fitchett Claude Chelala Michele Beaconsfield Edel A. O’Toole Michael P. Philpott Daniel G. Ezra
author_sort	John C. Bladen
title	MicroRNA and transcriptome analysis in periocular Sebaceous Gland Carcinoma
title_short	MicroRNA and transcriptome analysis in periocular Sebaceous Gland Carcinoma
title_full	MicroRNA and transcriptome analysis in periocular Sebaceous Gland Carcinoma
title_fullStr	MicroRNA and transcriptome analysis in periocular Sebaceous Gland Carcinoma
title_full_unstemmed	MicroRNA and transcriptome analysis in periocular Sebaceous Gland Carcinoma
title_sort	microrna and transcriptome analysis in periocular sebaceous gland carcinoma
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a8dbd7b7e4544abea6e78840cdc42587
work_keys_str_mv	AT johncbladen micrornaandtranscriptomeanalysisinperiocularsebaceousglandcarcinoma AT junwang micrornaandtranscriptomeanalysisinperiocularsebaceousglandcarcinoma AT ajanthahsangaralingam micrornaandtranscriptomeanalysisinperiocularsebaceousglandcarcinoma AT mariyamoosajee micrornaandtranscriptomeanalysisinperiocularsebaceousglandcarcinoma AT carolinefitchett micrornaandtranscriptomeanalysisinperiocularsebaceousglandcarcinoma AT claudechelala micrornaandtranscriptomeanalysisinperiocularsebaceousglandcarcinoma AT michelebeaconsfield micrornaandtranscriptomeanalysisinperiocularsebaceousglandcarcinoma AT edelaotoole micrornaandtranscriptomeanalysisinperiocularsebaceousglandcarcinoma AT michaelpphilpott micrornaandtranscriptomeanalysisinperiocularsebaceousglandcarcinoma AT danielgezra micrornaandtranscriptomeanalysisinperiocularsebaceousglandcarcinoma
_version_	1718384677092327424

MicroRNA and transcriptome analysis in periocular Sebaceous Gland Carcinoma

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