Identification of Extracellular DNA-Binding Proteins in the Biofilm Matrix

ABSTRACT We developed a new approach that couples Southwestern blotting and mass spectrometry to discover proteins that bind extracellular DNA (eDNA) in bacterial biofilms. Using Staphylococcus aureus as a model pathogen, we identified proteins with known DNA-binding activity and uncovered a series...

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Bibliographic Details
Main Authors: Jeffrey S. Kavanaugh, Caralyn E. Flack, Jessica Lister, Erica B. Ricker, Carolyn B. Ibberson, Christian Jenul, Derek E. Moormeier, Elizabeth A. Delmain, Kenneth W. Bayles, Alexander R. Horswill
Format: article
Language:EN
Published: American Society for Microbiology 2019
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Online Access:https://doaj.org/article/a8e208da8e384b019ca1c75e6293f2c3
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