Immunomodulatory Effects of Herbal Compounds Quercetin and Curcumin on Cellular and Molecular Functions of Bovine-Milk-Isolated Neutrophils toward <i>Streptococcus agalactiae</i> Infection

Herbal phytochemicals featuring active ingredients including quercetin and curcumin have shown potential in treating human and animal diseases. The current study investigated their potential function in vitro for host immunomodulation associated with <i>Streptococcus agalactiae</i> subcl...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Purichaya Disbanchong, Wichayaporn Punmanee, Anyaphat Srithanasuwan, Noppason Pangprasit, Kanruethai Wongsawan, Witaya Suriyasathaporn, Phongsakorn Chuammitri
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	quercetin curcumin neutrophil <i>Streptococcus agalactiae</i> mastitis cow Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/a8e36f1b2cc8497c9410b0614b23100e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a8e36f1b2cc8497c9410b0614b23100e
record_format	dspace
spelling	oai:doaj.org-article:a8e36f1b2cc8497c9410b0614b23100e2021-11-25T16:20:32ZImmunomodulatory Effects of Herbal Compounds Quercetin and Curcumin on Cellular and Molecular Functions of Bovine-Milk-Isolated Neutrophils toward <i>Streptococcus agalactiae</i> Infection10.3390/ani111132862076-2615https://doaj.org/article/a8e36f1b2cc8497c9410b0614b23100e2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3286https://doaj.org/toc/2076-2615Herbal phytochemicals featuring active ingredients including quercetin and curcumin have shown potential in treating human and animal diseases. The current study investigated their potential function in vitro for host immunomodulation associated with <i>Streptococcus agalactiae</i> subclinical bovine mastitis via milk-isolated neutrophils. Our results showed a positive influence on cellular migration, reactive oxygen species (ROS) generation, phagocytosis, and bacterial killing as well as neutrophil extracellular traps (NETs) release. This study also highlighted several important molecular aspects of quercetin and curcumin in milk-isolated neutrophils. Gene expression analyses by RT-PCR revealed significant changes in the expression of proinflammatory cytokines (<i>IL1B</i>, <i>IL6</i>, and <i>TNF</i>), ROS (<i>CYBA</i>), phagocytosis (<i>LAMP1</i>), and migration (<i>RAC</i>). The expression levels of apoptotic genes or proteins in either pro-apoptosis (<i>CASP3</i> and <i>FAS</i>) or anti-apoptosis (<i>BCL2</i>, <i>BCL2L1</i>, and <i>CFLAR</i>) were significantly manipulated by the effects of either quercetin or curcumin. A principal component analysis (PCA) identified the superior benefit of quercetin supplementation for increasing both cellular and molecular functions in combating bacterial mastitis. Altogether, this study showed the existing and potential benefits of these test compounds; however, they should be explored further via in vivo studies.Purichaya DisbanchongWichayaporn PunmaneeAnyaphat SrithanasuwanNoppason PangprasitKanruethai WongsawanWitaya SuriyasathapornPhongsakorn ChuammitriMDPI AGarticlequercetincurcuminneutrophil<i>Streptococcus agalactiae</i>mastitiscowVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3286, p 3286 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	quercetin curcumin neutrophil <i>Streptococcus agalactiae</i> mastitis cow Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	quercetin curcumin neutrophil <i>Streptococcus agalactiae</i> mastitis cow Veterinary medicine SF600-1100 Zoology QL1-991 Purichaya Disbanchong Wichayaporn Punmanee Anyaphat Srithanasuwan Noppason Pangprasit Kanruethai Wongsawan Witaya Suriyasathaporn Phongsakorn Chuammitri Immunomodulatory Effects of Herbal Compounds Quercetin and Curcumin on Cellular and Molecular Functions of Bovine-Milk-Isolated Neutrophils toward <i>Streptococcus agalactiae</i> Infection
description	Herbal phytochemicals featuring active ingredients including quercetin and curcumin have shown potential in treating human and animal diseases. The current study investigated their potential function in vitro for host immunomodulation associated with <i>Streptococcus agalactiae</i> subclinical bovine mastitis via milk-isolated neutrophils. Our results showed a positive influence on cellular migration, reactive oxygen species (ROS) generation, phagocytosis, and bacterial killing as well as neutrophil extracellular traps (NETs) release. This study also highlighted several important molecular aspects of quercetin and curcumin in milk-isolated neutrophils. Gene expression analyses by RT-PCR revealed significant changes in the expression of proinflammatory cytokines (<i>IL1B</i>, <i>IL6</i>, and <i>TNF</i>), ROS (<i>CYBA</i>), phagocytosis (<i>LAMP1</i>), and migration (<i>RAC</i>). The expression levels of apoptotic genes or proteins in either pro-apoptosis (<i>CASP3</i> and <i>FAS</i>) or anti-apoptosis (<i>BCL2</i>, <i>BCL2L1</i>, and <i>CFLAR</i>) were significantly manipulated by the effects of either quercetin or curcumin. A principal component analysis (PCA) identified the superior benefit of quercetin supplementation for increasing both cellular and molecular functions in combating bacterial mastitis. Altogether, this study showed the existing and potential benefits of these test compounds; however, they should be explored further via in vivo studies.
format	article
author	Purichaya Disbanchong Wichayaporn Punmanee Anyaphat Srithanasuwan Noppason Pangprasit Kanruethai Wongsawan Witaya Suriyasathaporn Phongsakorn Chuammitri
author_facet	Purichaya Disbanchong Wichayaporn Punmanee Anyaphat Srithanasuwan Noppason Pangprasit Kanruethai Wongsawan Witaya Suriyasathaporn Phongsakorn Chuammitri
author_sort	Purichaya Disbanchong
title	Immunomodulatory Effects of Herbal Compounds Quercetin and Curcumin on Cellular and Molecular Functions of Bovine-Milk-Isolated Neutrophils toward <i>Streptococcus agalactiae</i> Infection
title_short	Immunomodulatory Effects of Herbal Compounds Quercetin and Curcumin on Cellular and Molecular Functions of Bovine-Milk-Isolated Neutrophils toward <i>Streptococcus agalactiae</i> Infection
title_full	Immunomodulatory Effects of Herbal Compounds Quercetin and Curcumin on Cellular and Molecular Functions of Bovine-Milk-Isolated Neutrophils toward <i>Streptococcus agalactiae</i> Infection
title_fullStr	Immunomodulatory Effects of Herbal Compounds Quercetin and Curcumin on Cellular and Molecular Functions of Bovine-Milk-Isolated Neutrophils toward <i>Streptococcus agalactiae</i> Infection
title_full_unstemmed	Immunomodulatory Effects of Herbal Compounds Quercetin and Curcumin on Cellular and Molecular Functions of Bovine-Milk-Isolated Neutrophils toward <i>Streptococcus agalactiae</i> Infection
title_sort	immunomodulatory effects of herbal compounds quercetin and curcumin on cellular and molecular functions of bovine-milk-isolated neutrophils toward <i>streptococcus agalactiae</i> infection
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a8e36f1b2cc8497c9410b0614b23100e
work_keys_str_mv	AT purichayadisbanchong immunomodulatoryeffectsofherbalcompoundsquercetinandcurcuminoncellularandmolecularfunctionsofbovinemilkisolatedneutrophilstowardistreptococcusagalactiaeiinfection AT wichayapornpunmanee immunomodulatoryeffectsofherbalcompoundsquercetinandcurcuminoncellularandmolecularfunctionsofbovinemilkisolatedneutrophilstowardistreptococcusagalactiaeiinfection AT anyaphatsrithanasuwan immunomodulatoryeffectsofherbalcompoundsquercetinandcurcuminoncellularandmolecularfunctionsofbovinemilkisolatedneutrophilstowardistreptococcusagalactiaeiinfection AT noppasonpangprasit immunomodulatoryeffectsofherbalcompoundsquercetinandcurcuminoncellularandmolecularfunctionsofbovinemilkisolatedneutrophilstowardistreptococcusagalactiaeiinfection AT kanruethaiwongsawan immunomodulatoryeffectsofherbalcompoundsquercetinandcurcuminoncellularandmolecularfunctionsofbovinemilkisolatedneutrophilstowardistreptococcusagalactiaeiinfection AT witayasuriyasathaporn immunomodulatoryeffectsofherbalcompoundsquercetinandcurcuminoncellularandmolecularfunctionsofbovinemilkisolatedneutrophilstowardistreptococcusagalactiaeiinfection AT phongsakornchuammitri immunomodulatoryeffectsofherbalcompoundsquercetinandcurcuminoncellularandmolecularfunctionsofbovinemilkisolatedneutrophilstowardistreptococcusagalactiaeiinfection
_version_	1718413217929101312

Immunomodulatory Effects of Herbal Compounds Quercetin and Curcumin on Cellular and Molecular Functions of Bovine-Milk-Isolated Neutrophils toward <i>Streptococcus agalactiae</i> Infection

Ejemplares similares