Drosophila TRPN(=NOMPC) channel localizes to the distal end of mechanosensory cilia.

<h4>Background</h4>A TRPN channel protein is essential for sensory transduction in insect mechanosensory neurons and in vertebrate hair cells. The Drosophila TRPN homolog, NOMPC, is required to generate mechanoreceptor potentials and currents in tactile bristles. NOMPC is also required,...

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Autores principales:	Jeongmi Lee, Sungjin Moon, Yoonseok Cha, Yun Doo Chung
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a8e8d5c7a95f407cb004096a0d237a1a
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spelling	oai:doaj.org-article:a8e8d5c7a95f407cb004096a0d237a1a2021-12-02T20:21:08ZDrosophila TRPN(=NOMPC) channel localizes to the distal end of mechanosensory cilia.1932-620310.1371/journal.pone.0011012https://doaj.org/article/a8e8d5c7a95f407cb004096a0d237a1a2010-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20543979/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>A TRPN channel protein is essential for sensory transduction in insect mechanosensory neurons and in vertebrate hair cells. The Drosophila TRPN homolog, NOMPC, is required to generate mechanoreceptor potentials and currents in tactile bristles. NOMPC is also required, together with a TRPV channel, for transduction by chordotonal neurons of the fly's antennal ear, but the TRPN or TRPV channels have distinct roles in transduction and in regulating active antennal mechanics. The evidence suggests that NOMPC is a primary mechanotransducer channel, but its subcellular location-key for understanding its exact role in transduction-has not yet been established.<h4>Methodology/principal findings</h4>Here, by immunostaining, we locate NOMPC at the tips of mechanosensory cilia in both external and chordotonal sensory neurons, as predicted for a mechanotransducer channel. In chordotonal neurons, the TRPN and TRPV channels are respectively segregated into distal and proximal ciliary zones. This zonal separation is demarcated by and requires the ciliary dilation, an intraciliary assembly of intraflagellar transport (IFT) proteins.<h4>Conclusions</h4>Our results provide a strong evidence for NOMPC as a primary transduction channel in Drosophila mechansensory organs. The data also reveals a structural basis for the model of auditory chordotonal transduction in which the TRPN and TRPV channels play sequential roles in generating and amplifying the receptor potential, but have opposing roles in regulating active ciliary motility.Jeongmi LeeSungjin MoonYoonseok ChaYun Doo ChungPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 6, p e11012 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jeongmi Lee Sungjin Moon Yoonseok Cha Yun Doo Chung Drosophila TRPN(=NOMPC) channel localizes to the distal end of mechanosensory cilia.
description	<h4>Background</h4>A TRPN channel protein is essential for sensory transduction in insect mechanosensory neurons and in vertebrate hair cells. The Drosophila TRPN homolog, NOMPC, is required to generate mechanoreceptor potentials and currents in tactile bristles. NOMPC is also required, together with a TRPV channel, for transduction by chordotonal neurons of the fly's antennal ear, but the TRPN or TRPV channels have distinct roles in transduction and in regulating active antennal mechanics. The evidence suggests that NOMPC is a primary mechanotransducer channel, but its subcellular location-key for understanding its exact role in transduction-has not yet been established.<h4>Methodology/principal findings</h4>Here, by immunostaining, we locate NOMPC at the tips of mechanosensory cilia in both external and chordotonal sensory neurons, as predicted for a mechanotransducer channel. In chordotonal neurons, the TRPN and TRPV channels are respectively segregated into distal and proximal ciliary zones. This zonal separation is demarcated by and requires the ciliary dilation, an intraciliary assembly of intraflagellar transport (IFT) proteins.<h4>Conclusions</h4>Our results provide a strong evidence for NOMPC as a primary transduction channel in Drosophila mechansensory organs. The data also reveals a structural basis for the model of auditory chordotonal transduction in which the TRPN and TRPV channels play sequential roles in generating and amplifying the receptor potential, but have opposing roles in regulating active ciliary motility.
format	article
author	Jeongmi Lee Sungjin Moon Yoonseok Cha Yun Doo Chung
author_facet	Jeongmi Lee Sungjin Moon Yoonseok Cha Yun Doo Chung
author_sort	Jeongmi Lee
title	Drosophila TRPN(=NOMPC) channel localizes to the distal end of mechanosensory cilia.
title_short	Drosophila TRPN(=NOMPC) channel localizes to the distal end of mechanosensory cilia.
title_full	Drosophila TRPN(=NOMPC) channel localizes to the distal end of mechanosensory cilia.
title_fullStr	Drosophila TRPN(=NOMPC) channel localizes to the distal end of mechanosensory cilia.
title_full_unstemmed	Drosophila TRPN(=NOMPC) channel localizes to the distal end of mechanosensory cilia.
title_sort	drosophila trpn(=nompc) channel localizes to the distal end of mechanosensory cilia.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/a8e8d5c7a95f407cb004096a0d237a1a
work_keys_str_mv	AT jeongmilee drosophilatrpnnompcchannellocalizestothedistalendofmechanosensorycilia AT sungjinmoon drosophilatrpnnompcchannellocalizestothedistalendofmechanosensorycilia AT yoonseokcha drosophilatrpnnompcchannellocalizestothedistalendofmechanosensorycilia AT yundoochung drosophilatrpnnompcchannellocalizestothedistalendofmechanosensorycilia
_version_	1718374183480590336

Drosophila TRPN(=NOMPC) channel localizes to the distal end of mechanosensory cilia.

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