Biosynthesis, characterization, and antimicrobial applications of silver nanoparticles

Priyanka Singh,1 Yeon Ju Kim,2 Hina Singh,1 Chao Wang,2 Kyu Hyon Hwang,3 Mohamed El-Agamy Farh,1 Deok Chun Yang1,2 1Department of Oriental Medicinal Material and Processing, 2Graduate School of Biotechnology and Ginseng Bank, College of Life Sciences, Kyung Hee University, Yongin, 3Gyeonggi-Do Agr...

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Autores principales:	Singh P, Kim YJ, Singh H, Wang C, Hwang KH, Farh ME, Yang DC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a8eb2ba8239a480da7cc4d20f6dd8f17
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spelling	oai:doaj.org-article:a8eb2ba8239a480da7cc4d20f6dd8f172021-12-02T05:10:44ZBiosynthesis, characterization, and antimicrobial applications of silver nanoparticles1178-2013https://doaj.org/article/a8eb2ba8239a480da7cc4d20f6dd8f172015-03-01T00:00:00Zhttp://www.dovepress.com/biosynthesis-characterization-and-antimicrobial-applications-of-silver-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Priyanka Singh,1 Yeon Ju Kim,2 Hina Singh,1 Chao Wang,2 Kyu Hyon Hwang,3 Mohamed El-Agamy Farh,1 Deok Chun Yang1,2 1Department of Oriental Medicinal Material and Processing, 2Graduate School of Biotechnology and Ginseng Bank, College of Life Sciences, Kyung Hee University, Yongin, 3Gyeonggi-Do Agricultural Research & Extension Services, Gyeonggi, Republic of Korea Abstract: In the present study, the strain Brevibacterium frigoritolerans DC2 was explored for the efficient and extracellular synthesis of silver nanoparticles. These biosynthesized silver nanoparticles were characterized by ultraviolet-visible spectrophotometry, which detected the formation of silver nanoparticles in the reaction mixture and showed a maximum absorbance at 420 nm. In addition, field emission transmission electron microscopy revealed the spherical shape of the nanoparticles. The dynamic light scattering results indicated the average particle size of the product was 97 nm with a 0.191 polydispersity index. Furthermore, the product was analyzed by energy dispersive X-ray spectroscopy, X-ray diffraction, and elemental mapping, which displayed the presence of elemental silver in the product. Moreover, on a medical platform, the product was checked against pathogenic microorganisms including Vibrio parahaemolyticus, Salmonella enterica, Bacillus anthracis, Bacillus cereus, Escherichia coli, and Candida albicans. The nanoparticles demonstrated antimicrobial activity against all of these pathogenic microorganisms. Additionally, the silver nanoparticles were evaluated for their combined effects with the commercial antibiotics lincomycin, oleandomycin, vancomycin, novobiocin, penicillin G, and rifampicin against these pathogenic microorganisms. These results indicated that the combination of antibiotics with biosynthesized silver nanoparticles enhanced the antimicrobial effects of antibiotics. Therefore, the current study is a demonstration of an efficient biological synthesis of silver nanoparticles by B. frigoritolerans DC2 and its effect on the enhancement of the antmicrobial efficacy of well-known commercial antibiotics. Keywords: Brevibacterium frigoritolerans, biosynthesis, silver nanoparticles, antimicrobial activity, synergistic effectSingh PKim YJSingh HWang CHwang KHFarh MEYang DCDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 2567-2577 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Singh P Kim YJ Singh H Wang C Hwang KH Farh ME Yang DC Biosynthesis, characterization, and antimicrobial applications of silver nanoparticles
description	Priyanka Singh,1 Yeon Ju Kim,2 Hina Singh,1 Chao Wang,2 Kyu Hyon Hwang,3 Mohamed El-Agamy Farh,1 Deok Chun Yang1,2 1Department of Oriental Medicinal Material and Processing, 2Graduate School of Biotechnology and Ginseng Bank, College of Life Sciences, Kyung Hee University, Yongin, 3Gyeonggi-Do Agricultural Research & Extension Services, Gyeonggi, Republic of Korea Abstract: In the present study, the strain Brevibacterium frigoritolerans DC2 was explored for the efficient and extracellular synthesis of silver nanoparticles. These biosynthesized silver nanoparticles were characterized by ultraviolet-visible spectrophotometry, which detected the formation of silver nanoparticles in the reaction mixture and showed a maximum absorbance at 420 nm. In addition, field emission transmission electron microscopy revealed the spherical shape of the nanoparticles. The dynamic light scattering results indicated the average particle size of the product was 97 nm with a 0.191 polydispersity index. Furthermore, the product was analyzed by energy dispersive X-ray spectroscopy, X-ray diffraction, and elemental mapping, which displayed the presence of elemental silver in the product. Moreover, on a medical platform, the product was checked against pathogenic microorganisms including Vibrio parahaemolyticus, Salmonella enterica, Bacillus anthracis, Bacillus cereus, Escherichia coli, and Candida albicans. The nanoparticles demonstrated antimicrobial activity against all of these pathogenic microorganisms. Additionally, the silver nanoparticles were evaluated for their combined effects with the commercial antibiotics lincomycin, oleandomycin, vancomycin, novobiocin, penicillin G, and rifampicin against these pathogenic microorganisms. These results indicated that the combination of antibiotics with biosynthesized silver nanoparticles enhanced the antimicrobial effects of antibiotics. Therefore, the current study is a demonstration of an efficient biological synthesis of silver nanoparticles by B. frigoritolerans DC2 and its effect on the enhancement of the antmicrobial efficacy of well-known commercial antibiotics. Keywords: Brevibacterium frigoritolerans, biosynthesis, silver nanoparticles, antimicrobial activity, synergistic effect
format	article
author	Singh P Kim YJ Singh H Wang C Hwang KH Farh ME Yang DC
author_facet	Singh P Kim YJ Singh H Wang C Hwang KH Farh ME Yang DC
author_sort	Singh P
title	Biosynthesis, characterization, and antimicrobial applications of silver nanoparticles
title_short	Biosynthesis, characterization, and antimicrobial applications of silver nanoparticles
title_full	Biosynthesis, characterization, and antimicrobial applications of silver nanoparticles
title_fullStr	Biosynthesis, characterization, and antimicrobial applications of silver nanoparticles
title_full_unstemmed	Biosynthesis, characterization, and antimicrobial applications of silver nanoparticles
title_sort	biosynthesis, characterization, and antimicrobial applications of silver nanoparticles
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/a8eb2ba8239a480da7cc4d20f6dd8f17
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Biosynthesis, characterization, and antimicrobial applications of silver nanoparticles

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