Fabrication of Submicro-Nano Structures on Polyetheretherketone Surface by Femtosecond Laser for Exciting Cellular Responses of MC3T3-E1 Cells/Gingival Epithelial Cells

Dong Xie,1,2,* Chenhui Xu,1,* Cheng Ye,1,* Shiqi Mei,3 Longqing Wang,1 Qi Zhu,1 Qing Chen,1 Qi Zhao,1 Zhiyan Xu,3 Jie Wei,3 Lili Yang1 1Spine Center, Department of Orthopaedics, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, 200003, People’s Republic of China;...

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Autores principales:	Xie D, Xu C, Ye C, Mei S, Wang L, Zhu Q, Chen Q, Zhao Q, Xu Z, Wei J, Yang L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	polyetheretherketone surface modification submicro-nano structures functional group cell responses Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a8ee7681dbaf4be49f1ecf7f13ab8b3f
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spelling	oai:doaj.org-article:a8ee7681dbaf4be49f1ecf7f13ab8b3f2021-12-02T14:43:39ZFabrication of Submicro-Nano Structures on Polyetheretherketone Surface by Femtosecond Laser for Exciting Cellular Responses of MC3T3-E1 Cells/Gingival Epithelial Cells1178-2013https://doaj.org/article/a8ee7681dbaf4be49f1ecf7f13ab8b3f2021-05-01T00:00:00Zhttps://www.dovepress.com/fabrication-of-submicro-nano-structures-on-polyetheretherketone-surfac-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Dong Xie,1,2,* Chenhui Xu,1,* Cheng Ye,1,* Shiqi Mei,3 Longqing Wang,1 Qi Zhu,1 Qing Chen,1 Qi Zhao,1 Zhiyan Xu,3 Jie Wei,3 Lili Yang1 1Spine Center, Department of Orthopaedics, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, 200003, People’s Republic of China; 2Department of Orthopaedics, PLA Navy No.905 Hospital, Shanghai, 200052, People’s Republic of China; 3Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China*These authors contributed equally to this workCorrespondence: Lili Yang Tel +86-021-81885793Email yangll@smmu.edu.cnPurpose: Polyetheretherketone (PEEK) exhibits high mechanical strengths and outstanding biocompatibility but biological inertness that does not excite the cell responses and stimulate bone formation. The objective of this study was to construct submicro-nano structures on PEEK by femtosecond laser (FSL) for exciting the responses of MC3T3-E1 cells and gingival epithelial (GE) cells, which induce regeneration of bone/gingival tissues for long-term stability of dental implants.Materials and Methods: In this study, submicro-nano structures were created on PEEK surface by FSL with power of 80 mW (80FPK) and 160 mW (160FPK).Results: Compared with PEEK, both 80FPK and 160FPK with submicro-nano structures exhibited elevated surface performances (hydrophilicity, surface energy, roughness and protein absorption). Furthermore, in comparison with 80FPK, 160FPK further enhanced the surface performances. In addition, compared with PEEK, both 80FPK and 160FPK significantly excited not only the responses (adhesion, proliferation, alkaline phosphatase [ALP] activity and osteogenic gene expression) of MC3T3-E1 cells but also responses (adhesion as well as proliferation) of GE cells of human in vitro. Moreover, in comparison with 80FPK, 160FPK further enhanced the responses of MC3T3-E1 cells/GE cells.Conclusion: FSL created submicro-nano structures on PEEK with elevated surface performances, which played crucial roles in exciting the responses of MC3T3-E1 cells/GE cells. Consequently, 160FPK with elevated surface performances and outstanding cytocompatibility would have enormous potential as an implant for dental replacement.Keywords: polyetheretherketone; PEEK, surface modification, submicro-nano structures, functional group, cell responsesXie DXu CYe CMei SWang LZhu QChen QZhao QXu ZWei JYang LDove Medical Pressarticlepolyetheretherketonesurface modificationsubmicro-nano structuresfunctional groupcell responsesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 3201-3216 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyetheretherketone surface modification submicro-nano structures functional group cell responses Medicine (General) R5-920
spellingShingle	polyetheretherketone surface modification submicro-nano structures functional group cell responses Medicine (General) R5-920 Xie D Xu C Ye C Mei S Wang L Zhu Q Chen Q Zhao Q Xu Z Wei J Yang L Fabrication of Submicro-Nano Structures on Polyetheretherketone Surface by Femtosecond Laser for Exciting Cellular Responses of MC3T3-E1 Cells/Gingival Epithelial Cells
description	Dong Xie,1,2,* Chenhui Xu,1,* Cheng Ye,1,* Shiqi Mei,3 Longqing Wang,1 Qi Zhu,1 Qing Chen,1 Qi Zhao,1 Zhiyan Xu,3 Jie Wei,3 Lili Yang1 1Spine Center, Department of Orthopaedics, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, 200003, People’s Republic of China; 2Department of Orthopaedics, PLA Navy No.905 Hospital, Shanghai, 200052, People’s Republic of China; 3Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China*These authors contributed equally to this workCorrespondence: Lili Yang Tel +86-021-81885793Email yangll@smmu.edu.cnPurpose: Polyetheretherketone (PEEK) exhibits high mechanical strengths and outstanding biocompatibility but biological inertness that does not excite the cell responses and stimulate bone formation. The objective of this study was to construct submicro-nano structures on PEEK by femtosecond laser (FSL) for exciting the responses of MC3T3-E1 cells and gingival epithelial (GE) cells, which induce regeneration of bone/gingival tissues for long-term stability of dental implants.Materials and Methods: In this study, submicro-nano structures were created on PEEK surface by FSL with power of 80 mW (80FPK) and 160 mW (160FPK).Results: Compared with PEEK, both 80FPK and 160FPK with submicro-nano structures exhibited elevated surface performances (hydrophilicity, surface energy, roughness and protein absorption). Furthermore, in comparison with 80FPK, 160FPK further enhanced the surface performances. In addition, compared with PEEK, both 80FPK and 160FPK significantly excited not only the responses (adhesion, proliferation, alkaline phosphatase [ALP] activity and osteogenic gene expression) of MC3T3-E1 cells but also responses (adhesion as well as proliferation) of GE cells of human in vitro. Moreover, in comparison with 80FPK, 160FPK further enhanced the responses of MC3T3-E1 cells/GE cells.Conclusion: FSL created submicro-nano structures on PEEK with elevated surface performances, which played crucial roles in exciting the responses of MC3T3-E1 cells/GE cells. Consequently, 160FPK with elevated surface performances and outstanding cytocompatibility would have enormous potential as an implant for dental replacement.Keywords: polyetheretherketone; PEEK, surface modification, submicro-nano structures, functional group, cell responses
format	article
author	Xie D Xu C Ye C Mei S Wang L Zhu Q Chen Q Zhao Q Xu Z Wei J Yang L
author_facet	Xie D Xu C Ye C Mei S Wang L Zhu Q Chen Q Zhao Q Xu Z Wei J Yang L
author_sort	Xie D
title	Fabrication of Submicro-Nano Structures on Polyetheretherketone Surface by Femtosecond Laser for Exciting Cellular Responses of MC3T3-E1 Cells/Gingival Epithelial Cells
title_short	Fabrication of Submicro-Nano Structures on Polyetheretherketone Surface by Femtosecond Laser for Exciting Cellular Responses of MC3T3-E1 Cells/Gingival Epithelial Cells
title_full	Fabrication of Submicro-Nano Structures on Polyetheretherketone Surface by Femtosecond Laser for Exciting Cellular Responses of MC3T3-E1 Cells/Gingival Epithelial Cells
title_fullStr	Fabrication of Submicro-Nano Structures on Polyetheretherketone Surface by Femtosecond Laser for Exciting Cellular Responses of MC3T3-E1 Cells/Gingival Epithelial Cells
title_full_unstemmed	Fabrication of Submicro-Nano Structures on Polyetheretherketone Surface by Femtosecond Laser for Exciting Cellular Responses of MC3T3-E1 Cells/Gingival Epithelial Cells
title_sort	fabrication of submicro-nano structures on polyetheretherketone surface by femtosecond laser for exciting cellular responses of mc3t3-e1 cells/gingival epithelial cells
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/a8ee7681dbaf4be49f1ecf7f13ab8b3f
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Fabrication of Submicro-Nano Structures on Polyetheretherketone Surface by Femtosecond Laser for Exciting Cellular Responses of MC3T3-E1 Cells/Gingival Epithelial Cells

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