Suppression of VAMP2 Alters Morphology of the Tegument and Affects Glucose uptake, Development and Reproduction of Schistosoma japonicum

Abstract Schistosomiasis caused by schsitosomes is a serious global public health concern. The tegument that surrounds the worm is critical to the schistosomes survival. The tegument apical membrane undergoes a continuous process of rupture and repair owing to membranous vacuoles fusing with the pla...

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Autores principales: Qian Han, Bingguang Jia, Yang Hong, Xiaodan Cao, Qi Zhai, Ke Lu, Hao Li, Chuangang Zhu, Zhiqiang Fu, Yonghong Shi, Jiaojiao Lin
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Publicado: Nature Portfolio 2017
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spelling oai:doaj.org-article:a8f0c993c0f048c1910b4bd3e7ed30f42021-12-02T15:05:22ZSuppression of VAMP2 Alters Morphology of the Tegument and Affects Glucose uptake, Development and Reproduction of Schistosoma japonicum10.1038/s41598-017-05602-82045-2322https://doaj.org/article/a8f0c993c0f048c1910b4bd3e7ed30f42017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05602-8https://doaj.org/toc/2045-2322Abstract Schistosomiasis caused by schsitosomes is a serious global public health concern. The tegument that surrounds the worm is critical to the schistosomes survival. The tegument apical membrane undergoes a continuous process of rupture and repair owing to membranous vacuoles fusing with the plasma membrane. Vesicle-associated membrane protein 2 (VAMP2), a member of soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNAREs) is required for membrane fusion. Here, we used RNA interference (RNAi) to knock down the expression of VAMP2 of Schistosoma japonicum (SjVAMP2), and both real-time PCR and western blot analysis confirmed the suppression of this molecule, as well as the suppression of the transcript levels of schistosome glucose transporters (SGTP1 and SGTP4), and insulin receptors (SjIR1 and SjIR2). SjVAMP2-suppressed worms exhibited a lower viability, and phenotypic alterations were also observed in the tegument. Moreover, the glucose consumption of SjVAMP2-suppressed worms decreased significantly in 4 and 6 days, respectively, as well as a significant reduction in egg production. We also observed a significant reduction in worm burden and hepatic eggs burden in two independent RNAi experiment in vivo, and minor pathological changes in mice treated with SjVAMP2 specific small interfering (si)RNA. These findings reveal that SjVAMP2 may play important roles in the maintenance of tegument, glucose uptake, worm development and egg production in schistosomes.Qian HanBingguang JiaYang HongXiaodan CaoQi ZhaiKe LuHao LiChuangang ZhuZhiqiang FuYonghong ShiJiaojiao LinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Qian Han
Bingguang Jia
Yang Hong
Xiaodan Cao
Qi Zhai
Ke Lu
Hao Li
Chuangang Zhu
Zhiqiang Fu
Yonghong Shi
Jiaojiao Lin
Suppression of VAMP2 Alters Morphology of the Tegument and Affects Glucose uptake, Development and Reproduction of Schistosoma japonicum
description Abstract Schistosomiasis caused by schsitosomes is a serious global public health concern. The tegument that surrounds the worm is critical to the schistosomes survival. The tegument apical membrane undergoes a continuous process of rupture and repair owing to membranous vacuoles fusing with the plasma membrane. Vesicle-associated membrane protein 2 (VAMP2), a member of soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNAREs) is required for membrane fusion. Here, we used RNA interference (RNAi) to knock down the expression of VAMP2 of Schistosoma japonicum (SjVAMP2), and both real-time PCR and western blot analysis confirmed the suppression of this molecule, as well as the suppression of the transcript levels of schistosome glucose transporters (SGTP1 and SGTP4), and insulin receptors (SjIR1 and SjIR2). SjVAMP2-suppressed worms exhibited a lower viability, and phenotypic alterations were also observed in the tegument. Moreover, the glucose consumption of SjVAMP2-suppressed worms decreased significantly in 4 and 6 days, respectively, as well as a significant reduction in egg production. We also observed a significant reduction in worm burden and hepatic eggs burden in two independent RNAi experiment in vivo, and minor pathological changes in mice treated with SjVAMP2 specific small interfering (si)RNA. These findings reveal that SjVAMP2 may play important roles in the maintenance of tegument, glucose uptake, worm development and egg production in schistosomes.
format article
author Qian Han
Bingguang Jia
Yang Hong
Xiaodan Cao
Qi Zhai
Ke Lu
Hao Li
Chuangang Zhu
Zhiqiang Fu
Yonghong Shi
Jiaojiao Lin
author_facet Qian Han
Bingguang Jia
Yang Hong
Xiaodan Cao
Qi Zhai
Ke Lu
Hao Li
Chuangang Zhu
Zhiqiang Fu
Yonghong Shi
Jiaojiao Lin
author_sort Qian Han
title Suppression of VAMP2 Alters Morphology of the Tegument and Affects Glucose uptake, Development and Reproduction of Schistosoma japonicum
title_short Suppression of VAMP2 Alters Morphology of the Tegument and Affects Glucose uptake, Development and Reproduction of Schistosoma japonicum
title_full Suppression of VAMP2 Alters Morphology of the Tegument and Affects Glucose uptake, Development and Reproduction of Schistosoma japonicum
title_fullStr Suppression of VAMP2 Alters Morphology of the Tegument and Affects Glucose uptake, Development and Reproduction of Schistosoma japonicum
title_full_unstemmed Suppression of VAMP2 Alters Morphology of the Tegument and Affects Glucose uptake, Development and Reproduction of Schistosoma japonicum
title_sort suppression of vamp2 alters morphology of the tegument and affects glucose uptake, development and reproduction of schistosoma japonicum
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/a8f0c993c0f048c1910b4bd3e7ed30f4
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