Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens

Abstract The conversion of carbon dioxide to formate is a fundamental step for building C1 chemical platforms. Methylobacterium extorquens AM1 was reported to show remarkable activity converting carbon dioxide into formate. Formate dehydrogenase 1 from M. extorquens AM1 (MeFDH1) was verified as the...

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Autores principales:	Jungho Jang, Byoung Wook Jeon, Yong Hwan Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a8f1e7425b714eaa886bcc70e5096478
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spelling	oai:doaj.org-article:a8f1e7425b714eaa886bcc70e50964782021-12-02T11:41:13ZBioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens10.1038/s41598-018-23924-z2045-2322https://doaj.org/article/a8f1e7425b714eaa886bcc70e50964782018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-23924-zhttps://doaj.org/toc/2045-2322Abstract The conversion of carbon dioxide to formate is a fundamental step for building C1 chemical platforms. Methylobacterium extorquens AM1 was reported to show remarkable activity converting carbon dioxide into formate. Formate dehydrogenase 1 from M. extorquens AM1 (MeFDH1) was verified as the key responsible enzyme for the conversion of carbon dioxide to formate in this study. Using a 2% methanol concentration for induction, microbial harboring the recombinant MeFDH1 expressing plasmid produced the highest concentration of formate (26.6 mM within 21 hours) in electrochemical reactor. 60 μM of sodium tungstate in the culture medium was optimal for the expression of recombinant MeFDH1 and production of formate (25.7 mM within 21 hours). The recombinant MeFDH1 expressing cells showed maximum formate productivity of 2.53 mM/g-wet cell/hr, which was 2.5 times greater than that of wild type. Thus, M. extorquens AM1 was successfully engineered by expressing MeFDH1 as recombinant enzyme to elevate the production of formate from CO2 after elucidating key responsible enzyme for the conversion of CO2 to formate.Jungho JangByoung Wook JeonYong Hwan KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jungho Jang Byoung Wook Jeon Yong Hwan Kim Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens
description	Abstract The conversion of carbon dioxide to formate is a fundamental step for building C1 chemical platforms. Methylobacterium extorquens AM1 was reported to show remarkable activity converting carbon dioxide into formate. Formate dehydrogenase 1 from M. extorquens AM1 (MeFDH1) was verified as the key responsible enzyme for the conversion of carbon dioxide to formate in this study. Using a 2% methanol concentration for induction, microbial harboring the recombinant MeFDH1 expressing plasmid produced the highest concentration of formate (26.6 mM within 21 hours) in electrochemical reactor. 60 μM of sodium tungstate in the culture medium was optimal for the expression of recombinant MeFDH1 and production of formate (25.7 mM within 21 hours). The recombinant MeFDH1 expressing cells showed maximum formate productivity of 2.53 mM/g-wet cell/hr, which was 2.5 times greater than that of wild type. Thus, M. extorquens AM1 was successfully engineered by expressing MeFDH1 as recombinant enzyme to elevate the production of formate from CO2 after elucidating key responsible enzyme for the conversion of CO2 to formate.
format	article
author	Jungho Jang Byoung Wook Jeon Yong Hwan Kim
author_facet	Jungho Jang Byoung Wook Jeon Yong Hwan Kim
author_sort	Jungho Jang
title	Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens
title_short	Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens
title_full	Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens
title_fullStr	Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens
title_full_unstemmed	Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens
title_sort	bioelectrochemical conversion of co2 to value added product formate using engineered methylobacterium extorquens
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a8f1e7425b714eaa886bcc70e5096478
work_keys_str_mv	AT junghojang bioelectrochemicalconversionofco2tovalueaddedproductformateusingengineeredmethylobacteriumextorquens AT byoungwookjeon bioelectrochemicalconversionofco2tovalueaddedproductformateusingengineeredmethylobacteriumextorquens AT yonghwankim bioelectrochemicalconversionofco2tovalueaddedproductformateusingengineeredmethylobacteriumextorquens
_version_	1718395417463357440

Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens

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