Lack of decorin expression by human bladder cancer cells offers new tools in the therapy of urothelial malignancies.

Decorin, a multifunctional small leucine-rich extracellular matrix proteoglycan, has been shown to possess potent antitumour activity. However, there is some uncertainty whether different cancer cells express decorin in addition to non-malignant stromal cells. In this study we clarified decorin expr...

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Autores principales:	Annele Sainio, Marie Nyman, Riikka Lund, Sanna Vuorikoski, Pia Boström, Matti Laato, Peter J Boström, Hannu Järveläinen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a8f389f87cce49a5b979f5dd3ea6fc5f
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spelling	oai:doaj.org-article:a8f389f87cce49a5b979f5dd3ea6fc5f2021-11-18T08:51:28ZLack of decorin expression by human bladder cancer cells offers new tools in the therapy of urothelial malignancies.1932-620310.1371/journal.pone.0076190https://doaj.org/article/a8f389f87cce49a5b979f5dd3ea6fc5f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24146840/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Decorin, a multifunctional small leucine-rich extracellular matrix proteoglycan, has been shown to possess potent antitumour activity. However, there is some uncertainty whether different cancer cells express decorin in addition to non-malignant stromal cells. In this study we clarified decorin expression by human bladder cancer cells both in vivo and in vitro. In addition, the effect of adenovirus-mediated decorin expression on human bladder cancer cells in vitro was examined. We first demonstrated using the publicly available GeneSapiens databank that decorin gene expression is present in both normal and malignant human bladder tissues. However, when we applied in situ hybridization with digoxigenin-labeled RNA probes for decorin on human bladder carcinoma tissue samples derived from a large radical cystectomy patient cohort (n = 199), we unambiguously demonstrated that invasive and non-invasive bladder carcinoma cells completely lack decorin mRNA. The cancer cells were also negative for decorin immunoreactivity. Instead, decorin expression was localized solely to original non-malignant stromal areas of bladder tissue. In accordance with the aforementioned results, human bladder cancer cells in vitro were also negative for decorin expression as shown by RT-qPCR analyses. The lack of decorin expression by bladder cancer cells was shown not to be due to the methylation of the proximal promoter region of the decorin gene. When bladder cancer cells were transfected with a decorin adenoviral vector, their proliferation was significantly decreased. In conclusion, we have shown that human bladder cancer cells are totally devoid of decorin expression. We have also shown that adenovirus-mediated decorin gene transduction of human bladder cancer cell lines markedly inhibits their proliferation. Thus, decorin gene delivery offers new potential therapeutic tools in urothelial malignancies.Annele SainioMarie NymanRiikka LundSanna VuorikoskiPia BoströmMatti LaatoPeter J BoströmHannu JärveläinenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e76190 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Annele Sainio Marie Nyman Riikka Lund Sanna Vuorikoski Pia Boström Matti Laato Peter J Boström Hannu Järveläinen Lack of decorin expression by human bladder cancer cells offers new tools in the therapy of urothelial malignancies.
description	Decorin, a multifunctional small leucine-rich extracellular matrix proteoglycan, has been shown to possess potent antitumour activity. However, there is some uncertainty whether different cancer cells express decorin in addition to non-malignant stromal cells. In this study we clarified decorin expression by human bladder cancer cells both in vivo and in vitro. In addition, the effect of adenovirus-mediated decorin expression on human bladder cancer cells in vitro was examined. We first demonstrated using the publicly available GeneSapiens databank that decorin gene expression is present in both normal and malignant human bladder tissues. However, when we applied in situ hybridization with digoxigenin-labeled RNA probes for decorin on human bladder carcinoma tissue samples derived from a large radical cystectomy patient cohort (n = 199), we unambiguously demonstrated that invasive and non-invasive bladder carcinoma cells completely lack decorin mRNA. The cancer cells were also negative for decorin immunoreactivity. Instead, decorin expression was localized solely to original non-malignant stromal areas of bladder tissue. In accordance with the aforementioned results, human bladder cancer cells in vitro were also negative for decorin expression as shown by RT-qPCR analyses. The lack of decorin expression by bladder cancer cells was shown not to be due to the methylation of the proximal promoter region of the decorin gene. When bladder cancer cells were transfected with a decorin adenoviral vector, their proliferation was significantly decreased. In conclusion, we have shown that human bladder cancer cells are totally devoid of decorin expression. We have also shown that adenovirus-mediated decorin gene transduction of human bladder cancer cell lines markedly inhibits their proliferation. Thus, decorin gene delivery offers new potential therapeutic tools in urothelial malignancies.
format	article
author	Annele Sainio Marie Nyman Riikka Lund Sanna Vuorikoski Pia Boström Matti Laato Peter J Boström Hannu Järveläinen
author_facet	Annele Sainio Marie Nyman Riikka Lund Sanna Vuorikoski Pia Boström Matti Laato Peter J Boström Hannu Järveläinen
author_sort	Annele Sainio
title	Lack of decorin expression by human bladder cancer cells offers new tools in the therapy of urothelial malignancies.
title_short	Lack of decorin expression by human bladder cancer cells offers new tools in the therapy of urothelial malignancies.
title_full	Lack of decorin expression by human bladder cancer cells offers new tools in the therapy of urothelial malignancies.
title_fullStr	Lack of decorin expression by human bladder cancer cells offers new tools in the therapy of urothelial malignancies.
title_full_unstemmed	Lack of decorin expression by human bladder cancer cells offers new tools in the therapy of urothelial malignancies.
title_sort	lack of decorin expression by human bladder cancer cells offers new tools in the therapy of urothelial malignancies.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a8f389f87cce49a5b979f5dd3ea6fc5f
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Lack of decorin expression by human bladder cancer cells offers new tools in the therapy of urothelial malignancies.

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