Reshaping of the gastrointestinal microbiome alters atherosclerotic plaque inflammation resolution in mice

Abstract Since alterations in the intestinal microbiota may induce systemic inflammation and polarization of macrophages to the M1 state, the microbiome role in atherosclerosis, an M1-driven disease, requires evaluation. We aimed to determine if antibiotic (Abx) induced alterations to the intestinal...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Michael S. Garshick, Cyrus Nikain, Michael Tawil, Stephanie Pena, Tessa J. Barrett, Benjamin G. Wu, Zhan Gao, Martin J. Blaser, Edward A. Fisher
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a8f3dfa3b18f42a5b4b2ebab1cb9df7e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a8f3dfa3b18f42a5b4b2ebab1cb9df7e
record_format	dspace
spelling	oai:doaj.org-article:a8f3dfa3b18f42a5b4b2ebab1cb9df7e2021-12-02T13:40:51ZReshaping of the gastrointestinal microbiome alters atherosclerotic plaque inflammation resolution in mice10.1038/s41598-021-88479-y2045-2322https://doaj.org/article/a8f3dfa3b18f42a5b4b2ebab1cb9df7e2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88479-yhttps://doaj.org/toc/2045-2322Abstract Since alterations in the intestinal microbiota may induce systemic inflammation and polarization of macrophages to the M1 state, the microbiome role in atherosclerosis, an M1-driven disease, requires evaluation. We aimed to determine if antibiotic (Abx) induced alterations to the intestinal microbiota interferes with atherosclerotic plaque inflammation resolution after lipid-lowering in mice. Hyperlipidemic Apoe −/− mice were fed a western diet to develop aortic atherosclerosis with aortas then transplanted into normolipidemic wild-type (WT) mice to model clinically aggressive lipid management and promote atherosclerosis inflammation resolution. Gut microbial composition pre and post-transplant was altered via an enteral antibiotic or not. Post aortic transplant, after Abx treatment, while plaque size did not differ, compared to Apoe −/− mice, Abx– WT recipient mice had a 32% reduction in CD68-expressing cells (p = 0.02) vs. a non-significant 12% reduction in Abx+ WT mice. A trend toward an M1 plaque CD68-expresing cell phenotype was noted in Abx+ mice. By 16S rRNA sequence analysis, the Abx+ mice had reduced alpha diversity and increased Firmicutes/Bacteroidetes relative abundance ratio with a correlation between gut Firmicutes abundance and plaque CD68-expressing cell content (p < 0.05). These results indicate that in a murine atherosclerotic plaque inflammation resolution model, antibiotic-induced microbiome perturbation may blunt the effectiveness of lipid-lowering to reduce the content of plaque inflammatory CD68-expressing cells.Michael S. GarshickCyrus NikainMichael TawilStephanie PenaTessa J. BarrettBenjamin G. WuZhan GaoMartin J. BlaserEdward A. FisherNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michael S. Garshick Cyrus Nikain Michael Tawil Stephanie Pena Tessa J. Barrett Benjamin G. Wu Zhan Gao Martin J. Blaser Edward A. Fisher Reshaping of the gastrointestinal microbiome alters atherosclerotic plaque inflammation resolution in mice
description	Abstract Since alterations in the intestinal microbiota may induce systemic inflammation and polarization of macrophages to the M1 state, the microbiome role in atherosclerosis, an M1-driven disease, requires evaluation. We aimed to determine if antibiotic (Abx) induced alterations to the intestinal microbiota interferes with atherosclerotic plaque inflammation resolution after lipid-lowering in mice. Hyperlipidemic Apoe −/− mice were fed a western diet to develop aortic atherosclerosis with aortas then transplanted into normolipidemic wild-type (WT) mice to model clinically aggressive lipid management and promote atherosclerosis inflammation resolution. Gut microbial composition pre and post-transplant was altered via an enteral antibiotic or not. Post aortic transplant, after Abx treatment, while plaque size did not differ, compared to Apoe −/− mice, Abx– WT recipient mice had a 32% reduction in CD68-expressing cells (p = 0.02) vs. a non-significant 12% reduction in Abx+ WT mice. A trend toward an M1 plaque CD68-expresing cell phenotype was noted in Abx+ mice. By 16S rRNA sequence analysis, the Abx+ mice had reduced alpha diversity and increased Firmicutes/Bacteroidetes relative abundance ratio with a correlation between gut Firmicutes abundance and plaque CD68-expressing cell content (p < 0.05). These results indicate that in a murine atherosclerotic plaque inflammation resolution model, antibiotic-induced microbiome perturbation may blunt the effectiveness of lipid-lowering to reduce the content of plaque inflammatory CD68-expressing cells.
format	article
author	Michael S. Garshick Cyrus Nikain Michael Tawil Stephanie Pena Tessa J. Barrett Benjamin G. Wu Zhan Gao Martin J. Blaser Edward A. Fisher
author_facet	Michael S. Garshick Cyrus Nikain Michael Tawil Stephanie Pena Tessa J. Barrett Benjamin G. Wu Zhan Gao Martin J. Blaser Edward A. Fisher
author_sort	Michael S. Garshick
title	Reshaping of the gastrointestinal microbiome alters atherosclerotic plaque inflammation resolution in mice
title_short	Reshaping of the gastrointestinal microbiome alters atherosclerotic plaque inflammation resolution in mice
title_full	Reshaping of the gastrointestinal microbiome alters atherosclerotic plaque inflammation resolution in mice
title_fullStr	Reshaping of the gastrointestinal microbiome alters atherosclerotic plaque inflammation resolution in mice
title_full_unstemmed	Reshaping of the gastrointestinal microbiome alters atherosclerotic plaque inflammation resolution in mice
title_sort	reshaping of the gastrointestinal microbiome alters atherosclerotic plaque inflammation resolution in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a8f3dfa3b18f42a5b4b2ebab1cb9df7e
work_keys_str_mv	AT michaelsgarshick reshapingofthegastrointestinalmicrobiomealtersatheroscleroticplaqueinflammationresolutioninmice AT cyrusnikain reshapingofthegastrointestinalmicrobiomealtersatheroscleroticplaqueinflammationresolutioninmice AT michaeltawil reshapingofthegastrointestinalmicrobiomealtersatheroscleroticplaqueinflammationresolutioninmice AT stephaniepena reshapingofthegastrointestinalmicrobiomealtersatheroscleroticplaqueinflammationresolutioninmice AT tessajbarrett reshapingofthegastrointestinalmicrobiomealtersatheroscleroticplaqueinflammationresolutioninmice AT benjamingwu reshapingofthegastrointestinalmicrobiomealtersatheroscleroticplaqueinflammationresolutioninmice AT zhangao reshapingofthegastrointestinalmicrobiomealtersatheroscleroticplaqueinflammationresolutioninmice AT martinjblaser reshapingofthegastrointestinalmicrobiomealtersatheroscleroticplaqueinflammationresolutioninmice AT edwardafisher reshapingofthegastrointestinalmicrobiomealtersatheroscleroticplaqueinflammationresolutioninmice
_version_	1718392637003661312

Reshaping of the gastrointestinal microbiome alters atherosclerotic plaque inflammation resolution in mice

Ejemplares similares