Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia

Canine monocytic ehrlichiosis (CME) is a widespread, tick-borne, canine disease, caused by an obligate intracellular bacterium, Ehrlichia canis. The main vector, a brown-dog tick, Rhipicephalus sanguineus, is widely distributed, especially in areas with tropic, subtropic, or Mediterranean climates (...

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Autores principales:	Petrov Elena Atanaskova, Celeska Irena, Popova Zagorka, Krstevski Kiril, Djadjovski Igor
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2021
Materias:	canine monocytic ehrlichiosis dog qpcr 16s rrna gene Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/a9139b1c7a8e48058ad6061b802817f8
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id	oai:doaj.org-article:a9139b1c7a8e48058ad6061b802817f8
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spelling	oai:doaj.org-article:a9139b1c7a8e48058ad6061b802817f82021-11-17T21:27:54ZMolecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia1820-744810.2478/acve-2021-0020https://doaj.org/article/a9139b1c7a8e48058ad6061b802817f82021-06-01T00:00:00Zhttps://doi.org/10.2478/acve-2021-0020https://doaj.org/toc/1820-7448Canine monocytic ehrlichiosis (CME) is a widespread, tick-borne, canine disease, caused by an obligate intracellular bacterium, Ehrlichia canis. The main vector, a brown-dog tick, Rhipicephalus sanguineus, is widely distributed, especially in areas with tropic, subtropic, or Mediterranean climates (Central and South America, Eastern and Western Asia, Africa, Australia and Southern Europe). The study performed in 2012, by Stefanovska et al., determined a seroprevalence of 18.7% of E. canis among the Macedonian dog population. Up to date, the presence of E. canis, using molecular diagnostic methods, has not been investigated in Macedonia. Therefore, this study aimed to confirm the presence of E. canis, in the pet-dog population on the territory of the city of Skopje, North Macedonia, using a highly sensitive multiplex Real-Time PCR method (qPCR). Whole blood samples from 80 dogs of different breeds and ages, with clinical symptoms of CME and positive serology result for the presence of antibodies against E.canis, were collected for analyses. Out of 80 dogs, 36 (45%) were found as positive. The present work reports the first molecular detection of E. canis in pet dogs on the territory of the city of Skopje, Macedonia.Petrov Elena AtanaskovaCeleska IrenaPopova ZagorkaKrstevski KirilDjadjovski IgorSciendoarticlecanine monocytic ehrlichiosisdogqpcr16s rrna geneVeterinary medicineSF600-1100ENActa Veterinaria, Vol 71, Iss 2, Pp 230-238 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	canine monocytic ehrlichiosis dog qpcr 16s rrna gene Veterinary medicine SF600-1100
spellingShingle	canine monocytic ehrlichiosis dog qpcr 16s rrna gene Veterinary medicine SF600-1100 Petrov Elena Atanaskova Celeska Irena Popova Zagorka Krstevski Kiril Djadjovski Igor Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia
description	Canine monocytic ehrlichiosis (CME) is a widespread, tick-borne, canine disease, caused by an obligate intracellular bacterium, Ehrlichia canis. The main vector, a brown-dog tick, Rhipicephalus sanguineus, is widely distributed, especially in areas with tropic, subtropic, or Mediterranean climates (Central and South America, Eastern and Western Asia, Africa, Australia and Southern Europe). The study performed in 2012, by Stefanovska et al., determined a seroprevalence of 18.7% of E. canis among the Macedonian dog population. Up to date, the presence of E. canis, using molecular diagnostic methods, has not been investigated in Macedonia. Therefore, this study aimed to confirm the presence of E. canis, in the pet-dog population on the territory of the city of Skopje, North Macedonia, using a highly sensitive multiplex Real-Time PCR method (qPCR). Whole blood samples from 80 dogs of different breeds and ages, with clinical symptoms of CME and positive serology result for the presence of antibodies against E.canis, were collected for analyses. Out of 80 dogs, 36 (45%) were found as positive. The present work reports the first molecular detection of E. canis in pet dogs on the territory of the city of Skopje, Macedonia.
format	article
author	Petrov Elena Atanaskova Celeska Irena Popova Zagorka Krstevski Kiril Djadjovski Igor
author_facet	Petrov Elena Atanaskova Celeska Irena Popova Zagorka Krstevski Kiril Djadjovski Igor
author_sort	Petrov Elena Atanaskova
title	Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia
title_short	Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia
title_full	Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia
title_fullStr	Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia
title_full_unstemmed	Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia
title_sort	molecular detection of ehrlichia canis in the pet-dog population in r. n. macedonia
publisher	Sciendo
publishDate	2021
url	https://doaj.org/article/a9139b1c7a8e48058ad6061b802817f8
work_keys_str_mv	AT petrovelenaatanaskova moleculardetectionofehrlichiacanisinthepetdogpopulationinrnmacedonia AT celeskairena moleculardetectionofehrlichiacanisinthepetdogpopulationinrnmacedonia AT popovazagorka moleculardetectionofehrlichiacanisinthepetdogpopulationinrnmacedonia AT krstevskikiril moleculardetectionofehrlichiacanisinthepetdogpopulationinrnmacedonia AT djadjovskiigor moleculardetectionofehrlichiacanisinthepetdogpopulationinrnmacedonia
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Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia

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