Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery

Abstract Neuromuscular junctions (NMJs) are specialized synapses that connect motor neurons to skeletal muscle fibers and orchestrate proper signal transmission from the nervous system to muscles. The efficient formation and maintenance of the postsynaptic machinery that contains acetylcholine recep...

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Autores principales:	Krzysztof M. Bernadzki, Marta Gawor, Marcin Pęziński, Paula Mazurek, Paweł Niewiadomski, Maria J. Rędowicz, Tomasz J. Prószyński
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a915312fded04c5d85d5331d3e7c5e38
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spelling	oai:doaj.org-article:a915312fded04c5d85d5331d3e7c5e382021-12-02T11:52:59ZLiprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery10.1038/s41598-017-09590-72045-2322https://doaj.org/article/a915312fded04c5d85d5331d3e7c5e382017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09590-7https://doaj.org/toc/2045-2322Abstract Neuromuscular junctions (NMJs) are specialized synapses that connect motor neurons to skeletal muscle fibers and orchestrate proper signal transmission from the nervous system to muscles. The efficient formation and maintenance of the postsynaptic machinery that contains acetylcholine receptors (AChR) are indispensable for proper NMJ function. Abnormalities in the organization of synaptic components often cause severe neuromuscular disorders, such as muscular dystrophy. The dystrophin-associated glycoprotein complex (DGC) was shown to play an important role in NMJ development. We recently identified liprin-α-1 as a novel binding partner for one of the cytoplasmic DGC components, α-dystrobrevin-1. In the present study, we performed a detailed analysis of localization and function of liprin-α-1 at the murine NMJ. We showed that liprin-α-1 localizes to both pre- and postsynaptic compartments at the NMJ, and its synaptic enrichment depends on the presence of the nerve. Using cultured muscle cells, we found that liprin-α-1 plays an important role in AChR clustering and the organization of cortical microtubules. Our studies provide novel insights into the function of liprin-α-1 at vertebrate neuromuscular synapses.Krzysztof M. BernadzkiMarta GaworMarcin PęzińskiPaula MazurekPaweł NiewiadomskiMaria J. RędowiczTomasz J. PrószyńskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Krzysztof M. Bernadzki Marta Gawor Marcin Pęziński Paula Mazurek Paweł Niewiadomski Maria J. Rędowicz Tomasz J. Prószyński Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery
description	Abstract Neuromuscular junctions (NMJs) are specialized synapses that connect motor neurons to skeletal muscle fibers and orchestrate proper signal transmission from the nervous system to muscles. The efficient formation and maintenance of the postsynaptic machinery that contains acetylcholine receptors (AChR) are indispensable for proper NMJ function. Abnormalities in the organization of synaptic components often cause severe neuromuscular disorders, such as muscular dystrophy. The dystrophin-associated glycoprotein complex (DGC) was shown to play an important role in NMJ development. We recently identified liprin-α-1 as a novel binding partner for one of the cytoplasmic DGC components, α-dystrobrevin-1. In the present study, we performed a detailed analysis of localization and function of liprin-α-1 at the murine NMJ. We showed that liprin-α-1 localizes to both pre- and postsynaptic compartments at the NMJ, and its synaptic enrichment depends on the presence of the nerve. Using cultured muscle cells, we found that liprin-α-1 plays an important role in AChR clustering and the organization of cortical microtubules. Our studies provide novel insights into the function of liprin-α-1 at vertebrate neuromuscular synapses.
format	article
author	Krzysztof M. Bernadzki Marta Gawor Marcin Pęziński Paula Mazurek Paweł Niewiadomski Maria J. Rędowicz Tomasz J. Prószyński
author_facet	Krzysztof M. Bernadzki Marta Gawor Marcin Pęziński Paula Mazurek Paweł Niewiadomski Maria J. Rędowicz Tomasz J. Prószyński
author_sort	Krzysztof M. Bernadzki
title	Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery
title_short	Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery
title_full	Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery
title_fullStr	Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery
title_full_unstemmed	Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery
title_sort	liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a915312fded04c5d85d5331d3e7c5e38
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Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery

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