Experimental effect of retinoic acids on apoptosis during the development of diabetic retinopathy

Nami Nishikiori1,2, Makoto Osanai2, Hideki Chiba2, Takashi Kojima2, Shuichiro Inatomi1,2, Hiroshi Ohguro1, Norimasa Sawada2Departments of 1Ophthalmology and 2Pathology, Sapporo Medical University School of MedicinePurpose: This study was conducted to investigate whether retinoic acids (RAs) had any...

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Autores principales:	Nami Nishikiori, Makoto Osanai, Hideki Chiba, Takashi Kojima, Shuichiro Inatomi, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2008
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/a915711595334b43912dece0dbfa1984
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spelling	oai:doaj.org-article:a915711595334b43912dece0dbfa19842021-12-02T05:47:23ZExperimental effect of retinoic acids on apoptosis during the development of diabetic retinopathy1177-54671177-5483https://doaj.org/article/a915711595334b43912dece0dbfa19842008-03-01T00:00:00Zhttp://www.dovepress.com/experimental-effect-of-retinoic-acids-on-apoptosis-during-the-developm-a357https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Nami Nishikiori1,2, Makoto Osanai2, Hideki Chiba2, Takashi Kojima2, Shuichiro Inatomi1,2, Hiroshi Ohguro1, Norimasa Sawada2Departments of 1Ophthalmology and 2Pathology, Sapporo Medical University School of MedicinePurpose: This study was conducted to investigate whether retinoic acids (RAs) had any effect on apoptosis during the development of diabetic retinopathy.Methods: To investigate whether RAs had any effect on apoptosis during the development of diabetic retinopathy, we housed 32 C57BL/6 male mice and induced diabetes in 24 by intra peritoneal injections of streptozotocin (STZ; Sigma, St Louis, MO) and treated 16 of the diabetic mice with the RAs, all-trans-retinoic acid (ATRA) (seven mice) and 4-[(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)carboxamido] benzoic acid (Am580) (nine mice). The other eight mice were used as diabetic controls. We then measured apoptosis in the retina by TdT-dUTP terminal nick-end labeling assay.Results: RAs inhibited the apoptosis of retinal cells in diabetic retinopathy. Many apoptotic cells were observed in retinas of the eight diabetic control mice (mean value and SD: 37.8 &plusmn; 6.9), whereas when diabetic mice were treated with RAs, the number of apoptotic cells significantly decreased (mean value and SD: 9.9 &plusmn; 6.4 for the seven ATRA-treated diabetic mice and 9.8 &plusmn; 5.9 for the nine Am580-treated diabetic mice) (p &lt; 0.05).Conclusion: Treatment with RAs decreases apoptosis during the development of diabetic retinopathy.Keywords: retinoic acids, apoptosis, diabetic retinopathy, glial cell line-derived neurotrophic factor Nami NishikioriMakoto OsanaiHideki ChibaTakashi KojimaShuichiro Inatomiet alDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2008, Iss Issue 1, Pp 233-235 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Nami Nishikiori Makoto Osanai Hideki Chiba Takashi Kojima Shuichiro Inatomi et al Experimental effect of retinoic acids on apoptosis during the development of diabetic retinopathy
description	Nami Nishikiori1,2, Makoto Osanai2, Hideki Chiba2, Takashi Kojima2, Shuichiro Inatomi1,2, Hiroshi Ohguro1, Norimasa Sawada2Departments of 1Ophthalmology and 2Pathology, Sapporo Medical University School of MedicinePurpose: This study was conducted to investigate whether retinoic acids (RAs) had any effect on apoptosis during the development of diabetic retinopathy.Methods: To investigate whether RAs had any effect on apoptosis during the development of diabetic retinopathy, we housed 32 C57BL/6 male mice and induced diabetes in 24 by intra peritoneal injections of streptozotocin (STZ; Sigma, St Louis, MO) and treated 16 of the diabetic mice with the RAs, all-trans-retinoic acid (ATRA) (seven mice) and 4-[(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)carboxamido] benzoic acid (Am580) (nine mice). The other eight mice were used as diabetic controls. We then measured apoptosis in the retina by TdT-dUTP terminal nick-end labeling assay.Results: RAs inhibited the apoptosis of retinal cells in diabetic retinopathy. Many apoptotic cells were observed in retinas of the eight diabetic control mice (mean value and SD: 37.8 &plusmn; 6.9), whereas when diabetic mice were treated with RAs, the number of apoptotic cells significantly decreased (mean value and SD: 9.9 &plusmn; 6.4 for the seven ATRA-treated diabetic mice and 9.8 &plusmn; 5.9 for the nine Am580-treated diabetic mice) (p &lt; 0.05).Conclusion: Treatment with RAs decreases apoptosis during the development of diabetic retinopathy.Keywords: retinoic acids, apoptosis, diabetic retinopathy, glial cell line-derived neurotrophic factor
format	article
author	Nami Nishikiori Makoto Osanai Hideki Chiba Takashi Kojima Shuichiro Inatomi et al
author_facet	Nami Nishikiori Makoto Osanai Hideki Chiba Takashi Kojima Shuichiro Inatomi et al
author_sort	Nami Nishikiori
title	Experimental effect of retinoic acids on apoptosis during the development of diabetic retinopathy
title_short	Experimental effect of retinoic acids on apoptosis during the development of diabetic retinopathy
title_full	Experimental effect of retinoic acids on apoptosis during the development of diabetic retinopathy
title_fullStr	Experimental effect of retinoic acids on apoptosis during the development of diabetic retinopathy
title_full_unstemmed	Experimental effect of retinoic acids on apoptosis during the development of diabetic retinopathy
title_sort	experimental effect of retinoic acids on apoptosis during the development of diabetic retinopathy
publisher	Dove Medical Press
publishDate	2008
url	https://doaj.org/article/a915711595334b43912dece0dbfa1984
work_keys_str_mv	AT naminishikiori experimentaleffectofretinoicacidsonapoptosisduringthedevelopmentofdiabeticretinopathy AT makotoosanai experimentaleffectofretinoicacidsonapoptosisduringthedevelopmentofdiabeticretinopathy AT hidekichiba experimentaleffectofretinoicacidsonapoptosisduringthedevelopmentofdiabeticretinopathy AT takashikojima experimentaleffectofretinoicacidsonapoptosisduringthedevelopmentofdiabeticretinopathy AT shuichiroinatomi experimentaleffectofretinoicacidsonapoptosisduringthedevelopmentofdiabeticretinopathy AT etal experimentaleffectofretinoicacidsonapoptosisduringthedevelopmentofdiabeticretinopathy
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Experimental effect of retinoic acids on apoptosis during the development of diabetic retinopathy

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