Local administration of AAV-BDNF to subventricular zone induces functional recovery in stroke rats.

Migration of new neuroprogenitor cells (NPCs) from the subventricular zone (SVZ) plays an important role in neurorepair after injury. Previous studies have shown that brain derived neurotrophic factor (BDNF) enhances the migration of NPCs from SVZ explants in neonatal mice in vitro. The purpose of t...

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Autores principales:	Seong-Jin Yu, Kuan-Yin Tseng, Hui Shen, Brandon K Harvey, Mikko Airavaara, Yun Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a918fc4d3d9b4866b76ef335b44c5913
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spelling	oai:doaj.org-article:a918fc4d3d9b4866b76ef335b44c59132021-11-18T08:43:55ZLocal administration of AAV-BDNF to subventricular zone induces functional recovery in stroke rats.1932-620310.1371/journal.pone.0081750https://doaj.org/article/a918fc4d3d9b4866b76ef335b44c59132013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24312581/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Migration of new neuroprogenitor cells (NPCs) from the subventricular zone (SVZ) plays an important role in neurorepair after injury. Previous studies have shown that brain derived neurotrophic factor (BDNF) enhances the migration of NPCs from SVZ explants in neonatal mice in vitro. The purpose of this study was to identify the role of BDNF in SVZ cells using AAV-BDNF in an animal model of stroke. BDNF protein production after AAV-BDNF infection was verified in primary neuronal culture. AAV-BDNF or AAV-RFP was injected into the left SVZ region of adult rats at 14 days prior to right middle cerebral artery occlusion (MCAo). SVZ tissues were collected from the brain and placed in Metrigel cultures 1 day after MCAo. Treatment with AAV-BDNF significantly increased the migration of SVZ cells in the stroke brain in vitro. In another set of animals, AAV-GFP was co-injected with AAV-BDNF or AAV-RFP to label cells in left SVZ prior to right MCAo. Local administration of AAV-BDNF significantly enhanced recovery of locomotor function and migration of GFP-positive cells from the SVZ toward the lesioned hemisphere in stroke rats. Our data suggest that focal administration of AAV-BDNF to the SVZ increases behavioral recovery post stroke, possibly through the enhancement of migration of cells from SVZ in stroke animals. Regional manipulation of BDNF expression through AAV may be a novel approach for neurorepair in stroke brains.Seong-Jin YuKuan-Yin TsengHui ShenBrandon K HarveyMikko AiravaaraYun WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e81750 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seong-Jin Yu Kuan-Yin Tseng Hui Shen Brandon K Harvey Mikko Airavaara Yun Wang Local administration of AAV-BDNF to subventricular zone induces functional recovery in stroke rats.
description	Migration of new neuroprogenitor cells (NPCs) from the subventricular zone (SVZ) plays an important role in neurorepair after injury. Previous studies have shown that brain derived neurotrophic factor (BDNF) enhances the migration of NPCs from SVZ explants in neonatal mice in vitro. The purpose of this study was to identify the role of BDNF in SVZ cells using AAV-BDNF in an animal model of stroke. BDNF protein production after AAV-BDNF infection was verified in primary neuronal culture. AAV-BDNF or AAV-RFP was injected into the left SVZ region of adult rats at 14 days prior to right middle cerebral artery occlusion (MCAo). SVZ tissues were collected from the brain and placed in Metrigel cultures 1 day after MCAo. Treatment with AAV-BDNF significantly increased the migration of SVZ cells in the stroke brain in vitro. In another set of animals, AAV-GFP was co-injected with AAV-BDNF or AAV-RFP to label cells in left SVZ prior to right MCAo. Local administration of AAV-BDNF significantly enhanced recovery of locomotor function and migration of GFP-positive cells from the SVZ toward the lesioned hemisphere in stroke rats. Our data suggest that focal administration of AAV-BDNF to the SVZ increases behavioral recovery post stroke, possibly through the enhancement of migration of cells from SVZ in stroke animals. Regional manipulation of BDNF expression through AAV may be a novel approach for neurorepair in stroke brains.
format	article
author	Seong-Jin Yu Kuan-Yin Tseng Hui Shen Brandon K Harvey Mikko Airavaara Yun Wang
author_facet	Seong-Jin Yu Kuan-Yin Tseng Hui Shen Brandon K Harvey Mikko Airavaara Yun Wang
author_sort	Seong-Jin Yu
title	Local administration of AAV-BDNF to subventricular zone induces functional recovery in stroke rats.
title_short	Local administration of AAV-BDNF to subventricular zone induces functional recovery in stroke rats.
title_full	Local administration of AAV-BDNF to subventricular zone induces functional recovery in stroke rats.
title_fullStr	Local administration of AAV-BDNF to subventricular zone induces functional recovery in stroke rats.
title_full_unstemmed	Local administration of AAV-BDNF to subventricular zone induces functional recovery in stroke rats.
title_sort	local administration of aav-bdnf to subventricular zone induces functional recovery in stroke rats.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a918fc4d3d9b4866b76ef335b44c5913
work_keys_str_mv	AT seongjinyu localadministrationofaavbdnftosubventricularzoneinducesfunctionalrecoveryinstrokerats AT kuanyintseng localadministrationofaavbdnftosubventricularzoneinducesfunctionalrecoveryinstrokerats AT huishen localadministrationofaavbdnftosubventricularzoneinducesfunctionalrecoveryinstrokerats AT brandonkharvey localadministrationofaavbdnftosubventricularzoneinducesfunctionalrecoveryinstrokerats AT mikkoairavaara localadministrationofaavbdnftosubventricularzoneinducesfunctionalrecoveryinstrokerats AT yunwang localadministrationofaavbdnftosubventricularzoneinducesfunctionalrecoveryinstrokerats
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Local administration of AAV-BDNF to subventricular zone induces functional recovery in stroke rats.

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