Tomato and cotton G protein beta subunit mutants display constitutive autoimmune responses

Abstract Heterotrimeric G protein Gβ‐deficient mutants in rice and maize display constitutive immune responses, whereas Arabidopsis Gβ mutants show impaired defense, suggesting the existence of functional differences between monocots and dicots. Using CRISPR/Cas9, we produced one hemizygous tomato l...

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Autores principales:	Thi Thao Ninh, Wei Gao, Yuri Trusov, Jing‐Ruo Zhao, Lu Long, Chun‐Peng Song, Jose Ramon Botella
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Botany QK1-989
Acceso en línea:	https://doaj.org/article/a9195c4ba12b4d479cfd80ec807999d6
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spelling	oai:doaj.org-article:a9195c4ba12b4d479cfd80ec807999d62021-11-29T07:25:55ZTomato and cotton G protein beta subunit mutants display constitutive autoimmune responses2475-445510.1002/pld3.359https://doaj.org/article/a9195c4ba12b4d479cfd80ec807999d62021-11-01T00:00:00Zhttps://doi.org/10.1002/pld3.359https://doaj.org/toc/2475-4455Abstract Heterotrimeric G protein Gβ‐deficient mutants in rice and maize display constitutive immune responses, whereas Arabidopsis Gβ mutants show impaired defense, suggesting the existence of functional differences between monocots and dicots. Using CRISPR/Cas9, we produced one hemizygous tomato line with a mutated SlGB1 Gβ gene. Homozygous slgb1 knockout mutants exhibit all the hallmarks of autoimmune mutants, including development of necrotic lesions, constitutive expression of defense‐related genes, and high endogenous levels of salicylic acid (SA) and reactive oxygen species, resulting in early seedling lethality. Virus‐induced silencing of Gβ in cotton reproduced the symptoms observed in tomato mutants, confirming that the autoimmune phenotype is not limited to monocot species but is also shared by dicots. Even though multiple genes involved in SA and ethylene signaling are highly induced by Gβ silencing in tomato and cotton, co‐silencing of SA or ethylene signaling components in cotton failed to suppress the lethal phenotype, whereas co‐silencing of the oxidative burst oxidase RbohD can repress lethality. Despite the autoimmune response observed in slgb1 mutants, we show that SlGB1 is a positive regulator of the pathogen‐associated molecular pattern (PAMP)‐triggered immunity (PTI) response in tomato. We speculate that the phenotypic differences observed between Arabidopsis and tomato/cotton/rice/maize Gβ knockouts do not necessarily reflect divergences in G protein‐mediated defense mechanisms.Thi Thao NinhWei GaoYuri TrusovJing‐Ruo ZhaoLu LongChun‐Peng SongJose Ramon BotellaWileyarticleBotanyQK1-989ENPlant Direct, Vol 5, Iss 11, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Botany QK1-989
spellingShingle	Botany QK1-989 Thi Thao Ninh Wei Gao Yuri Trusov Jing‐Ruo Zhao Lu Long Chun‐Peng Song Jose Ramon Botella Tomato and cotton G protein beta subunit mutants display constitutive autoimmune responses
description	Abstract Heterotrimeric G protein Gβ‐deficient mutants in rice and maize display constitutive immune responses, whereas Arabidopsis Gβ mutants show impaired defense, suggesting the existence of functional differences between monocots and dicots. Using CRISPR/Cas9, we produced one hemizygous tomato line with a mutated SlGB1 Gβ gene. Homozygous slgb1 knockout mutants exhibit all the hallmarks of autoimmune mutants, including development of necrotic lesions, constitutive expression of defense‐related genes, and high endogenous levels of salicylic acid (SA) and reactive oxygen species, resulting in early seedling lethality. Virus‐induced silencing of Gβ in cotton reproduced the symptoms observed in tomato mutants, confirming that the autoimmune phenotype is not limited to monocot species but is also shared by dicots. Even though multiple genes involved in SA and ethylene signaling are highly induced by Gβ silencing in tomato and cotton, co‐silencing of SA or ethylene signaling components in cotton failed to suppress the lethal phenotype, whereas co‐silencing of the oxidative burst oxidase RbohD can repress lethality. Despite the autoimmune response observed in slgb1 mutants, we show that SlGB1 is a positive regulator of the pathogen‐associated molecular pattern (PAMP)‐triggered immunity (PTI) response in tomato. We speculate that the phenotypic differences observed between Arabidopsis and tomato/cotton/rice/maize Gβ knockouts do not necessarily reflect divergences in G protein‐mediated defense mechanisms.
format	article
author	Thi Thao Ninh Wei Gao Yuri Trusov Jing‐Ruo Zhao Lu Long Chun‐Peng Song Jose Ramon Botella
author_facet	Thi Thao Ninh Wei Gao Yuri Trusov Jing‐Ruo Zhao Lu Long Chun‐Peng Song Jose Ramon Botella
author_sort	Thi Thao Ninh
title	Tomato and cotton G protein beta subunit mutants display constitutive autoimmune responses
title_short	Tomato and cotton G protein beta subunit mutants display constitutive autoimmune responses
title_full	Tomato and cotton G protein beta subunit mutants display constitutive autoimmune responses
title_fullStr	Tomato and cotton G protein beta subunit mutants display constitutive autoimmune responses
title_full_unstemmed	Tomato and cotton G protein beta subunit mutants display constitutive autoimmune responses
title_sort	tomato and cotton g protein beta subunit mutants display constitutive autoimmune responses
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/a9195c4ba12b4d479cfd80ec807999d6
work_keys_str_mv	AT thithaoninh tomatoandcottongproteinbetasubunitmutantsdisplayconstitutiveautoimmuneresponses AT weigao tomatoandcottongproteinbetasubunitmutantsdisplayconstitutiveautoimmuneresponses AT yuritrusov tomatoandcottongproteinbetasubunitmutantsdisplayconstitutiveautoimmuneresponses AT jingruozhao tomatoandcottongproteinbetasubunitmutantsdisplayconstitutiveautoimmuneresponses AT lulong tomatoandcottongproteinbetasubunitmutantsdisplayconstitutiveautoimmuneresponses AT chunpengsong tomatoandcottongproteinbetasubunitmutantsdisplayconstitutiveautoimmuneresponses AT joseramonbotella tomatoandcottongproteinbetasubunitmutantsdisplayconstitutiveautoimmuneresponses
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Tomato and cotton G protein beta subunit mutants display constitutive autoimmune responses

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