Specific features of human monocytes activation by monophosphoryl lipid A

Abstract We deciphered the mechanisms of production of pro- and anti-inflammatory cytokines by adherent human blood mononuclear cells (PBMC) activated by lipopolysaccharide (LPS) or monophosphoryl lipid A (MPLA). Both LPS and MPLA induced tumor necrosis factor (TNF) production proved to be dependent...

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Autores principales:	Ryme Chentouh, Catherine Fitting, Jean-Marc Cavaillon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a91c9f1d174d41a9abed67880fdeed44
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spelling	oai:doaj.org-article:a91c9f1d174d41a9abed67880fdeed442021-12-02T12:32:22ZSpecific features of human monocytes activation by monophosphoryl lipid A10.1038/s41598-018-25367-y2045-2322https://doaj.org/article/a91c9f1d174d41a9abed67880fdeed442018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25367-yhttps://doaj.org/toc/2045-2322Abstract We deciphered the mechanisms of production of pro- and anti-inflammatory cytokines by adherent human blood mononuclear cells (PBMC) activated by lipopolysaccharide (LPS) or monophosphoryl lipid A (MPLA). Both LPS and MPLA induced tumor necrosis factor (TNF) production proved to be dependent on the production of interleukin-1β (IL-1β). Of note, MPLA induced IL-1β release in human adherent PBMCs whereas MPLA was previously reported to not induce this cytokine in murine cells. Both LPS and MPLA stimulatory effects were inhibited by Toll-like receptor-4 (TLR4) antagonists. Only monocytes activation by LPS was dependent on CD14. Other differences were noticed between LPS and MPLA. Among the different donors, a strong correlation existed in terms of the levels of TNF induced by different LPSs. In contrast, there was no correlation between the TNF productions induced by LPS and those induced by MPLA. However, there was a strong correlation when IL-6 production was analyzed. Blocking actin polymerization and internalization of the agonists inhibited MPLA induced TNF production while the effect on LPS induced TNF production depended on the donors (i.e. high TNF producers versus low TNF producers). Finally, conventional LPS, tolerized adherent PBMCs to TLR2 agonists, while MPLA primed cells to further challenge with TLR2 agonists.Ryme ChentouhCatherine FittingJean-Marc CavaillonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryme Chentouh Catherine Fitting Jean-Marc Cavaillon Specific features of human monocytes activation by monophosphoryl lipid A
description	Abstract We deciphered the mechanisms of production of pro- and anti-inflammatory cytokines by adherent human blood mononuclear cells (PBMC) activated by lipopolysaccharide (LPS) or monophosphoryl lipid A (MPLA). Both LPS and MPLA induced tumor necrosis factor (TNF) production proved to be dependent on the production of interleukin-1β (IL-1β). Of note, MPLA induced IL-1β release in human adherent PBMCs whereas MPLA was previously reported to not induce this cytokine in murine cells. Both LPS and MPLA stimulatory effects were inhibited by Toll-like receptor-4 (TLR4) antagonists. Only monocytes activation by LPS was dependent on CD14. Other differences were noticed between LPS and MPLA. Among the different donors, a strong correlation existed in terms of the levels of TNF induced by different LPSs. In contrast, there was no correlation between the TNF productions induced by LPS and those induced by MPLA. However, there was a strong correlation when IL-6 production was analyzed. Blocking actin polymerization and internalization of the agonists inhibited MPLA induced TNF production while the effect on LPS induced TNF production depended on the donors (i.e. high TNF producers versus low TNF producers). Finally, conventional LPS, tolerized adherent PBMCs to TLR2 agonists, while MPLA primed cells to further challenge with TLR2 agonists.
format	article
author	Ryme Chentouh Catherine Fitting Jean-Marc Cavaillon
author_facet	Ryme Chentouh Catherine Fitting Jean-Marc Cavaillon
author_sort	Ryme Chentouh
title	Specific features of human monocytes activation by monophosphoryl lipid A
title_short	Specific features of human monocytes activation by monophosphoryl lipid A
title_full	Specific features of human monocytes activation by monophosphoryl lipid A
title_fullStr	Specific features of human monocytes activation by monophosphoryl lipid A
title_full_unstemmed	Specific features of human monocytes activation by monophosphoryl lipid A
title_sort	specific features of human monocytes activation by monophosphoryl lipid a
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a91c9f1d174d41a9abed67880fdeed44
work_keys_str_mv	AT rymechentouh specificfeaturesofhumanmonocytesactivationbymonophosphoryllipida AT catherinefitting specificfeaturesofhumanmonocytesactivationbymonophosphoryllipida AT jeanmarccavaillon specificfeaturesofhumanmonocytesactivationbymonophosphoryllipida
_version_	1718394112660471808

Specific features of human monocytes activation by monophosphoryl lipid A

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