Specific features of human monocytes activation by monophosphoryl lipid A

Abstract We deciphered the mechanisms of production of pro- and anti-inflammatory cytokines by adherent human blood mononuclear cells (PBMC) activated by lipopolysaccharide (LPS) or monophosphoryl lipid A (MPLA). Both LPS and MPLA induced tumor necrosis factor (TNF) production proved to be dependent...

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Auteurs principaux: Ryme Chentouh, Catherine Fitting, Jean-Marc Cavaillon
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Langue:EN
Publié: Nature Portfolio 2018
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spelling oai:doaj.org-article:a91c9f1d174d41a9abed67880fdeed442021-12-02T12:32:22ZSpecific features of human monocytes activation by monophosphoryl lipid A10.1038/s41598-018-25367-y2045-2322https://doaj.org/article/a91c9f1d174d41a9abed67880fdeed442018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25367-yhttps://doaj.org/toc/2045-2322Abstract We deciphered the mechanisms of production of pro- and anti-inflammatory cytokines by adherent human blood mononuclear cells (PBMC) activated by lipopolysaccharide (LPS) or monophosphoryl lipid A (MPLA). Both LPS and MPLA induced tumor necrosis factor (TNF) production proved to be dependent on the production of interleukin-1β (IL-1β). Of note, MPLA induced IL-1β release in human adherent PBMCs whereas MPLA was previously reported to not induce this cytokine in murine cells. Both LPS and MPLA stimulatory effects were inhibited by Toll-like receptor-4 (TLR4) antagonists. Only monocytes activation by LPS was dependent on CD14. Other differences were noticed between LPS and MPLA. Among the different donors, a strong correlation existed in terms of the levels of TNF induced by different LPSs. In contrast, there was no correlation between the TNF productions induced by LPS and those induced by MPLA. However, there was a strong correlation when IL-6 production was analyzed. Blocking actin polymerization and internalization of the agonists inhibited MPLA induced TNF production while the effect on LPS induced TNF production depended on the donors (i.e. high TNF producers versus low TNF producers). Finally, conventional LPS, tolerized adherent PBMCs to TLR2 agonists, while MPLA primed cells to further challenge with TLR2 agonists.Ryme ChentouhCatherine FittingJean-Marc CavaillonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Ryme Chentouh
Catherine Fitting
Jean-Marc Cavaillon
Specific features of human monocytes activation by monophosphoryl lipid A
description Abstract We deciphered the mechanisms of production of pro- and anti-inflammatory cytokines by adherent human blood mononuclear cells (PBMC) activated by lipopolysaccharide (LPS) or monophosphoryl lipid A (MPLA). Both LPS and MPLA induced tumor necrosis factor (TNF) production proved to be dependent on the production of interleukin-1β (IL-1β). Of note, MPLA induced IL-1β release in human adherent PBMCs whereas MPLA was previously reported to not induce this cytokine in murine cells. Both LPS and MPLA stimulatory effects were inhibited by Toll-like receptor-4 (TLR4) antagonists. Only monocytes activation by LPS was dependent on CD14. Other differences were noticed between LPS and MPLA. Among the different donors, a strong correlation existed in terms of the levels of TNF induced by different LPSs. In contrast, there was no correlation between the TNF productions induced by LPS and those induced by MPLA. However, there was a strong correlation when IL-6 production was analyzed. Blocking actin polymerization and internalization of the agonists inhibited MPLA induced TNF production while the effect on LPS induced TNF production depended on the donors (i.e. high TNF producers versus low TNF producers). Finally, conventional LPS, tolerized adherent PBMCs to TLR2 agonists, while MPLA primed cells to further challenge with TLR2 agonists.
format article
author Ryme Chentouh
Catherine Fitting
Jean-Marc Cavaillon
author_facet Ryme Chentouh
Catherine Fitting
Jean-Marc Cavaillon
author_sort Ryme Chentouh
title Specific features of human monocytes activation by monophosphoryl lipid A
title_short Specific features of human monocytes activation by monophosphoryl lipid A
title_full Specific features of human monocytes activation by monophosphoryl lipid A
title_fullStr Specific features of human monocytes activation by monophosphoryl lipid A
title_full_unstemmed Specific features of human monocytes activation by monophosphoryl lipid A
title_sort specific features of human monocytes activation by monophosphoryl lipid a
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/a91c9f1d174d41a9abed67880fdeed44
work_keys_str_mv AT rymechentouh specificfeaturesofhumanmonocytesactivationbymonophosphoryllipida
AT catherinefitting specificfeaturesofhumanmonocytesactivationbymonophosphoryllipida
AT jeanmarccavaillon specificfeaturesofhumanmonocytesactivationbymonophosphoryllipida
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