New generation of electrochemical immunoassay based on polymeric nanoparticles for early detection of breast cancer

Fouzi Mouffouk,1 Sihem Aouabdi,2 Entesar Al-Hetlani,1 Hacene Serrai,3 Tareq Alrefae,4 Liaohai Leo Chen5 1Department of Chemistry, Kuwait University, Safat, Kuwait; 2King Abdullah International Medical Research Center (KAIMRC), Jeddah, Kingdom of Saudi Arabia; 3Department of Radiology and Nuclear Me...

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Autores principales:	Mouffouk F, Aouabdi S, Al-Hetlani E, Serrai H, Alrefae T, Leo Chen L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Electrochemical immunoassay Polymeric nanoparticles Breast cancer biomarkers Biosensors. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a9244fdcd23143b2a3d604fba02686a2
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spelling	oai:doaj.org-article:a9244fdcd23143b2a3d604fba02686a22021-12-02T01:34:50ZNew generation of electrochemical immunoassay based on polymeric nanoparticles for early detection of breast cancer1178-2013https://doaj.org/article/a9244fdcd23143b2a3d604fba02686a22017-04-01T00:00:00Zhttps://www.dovepress.com/new-generation-of-electrochemical-immunoassay-based-on-polymeric-nanop-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Fouzi Mouffouk,1 Sihem Aouabdi,2 Entesar Al-Hetlani,1 Hacene Serrai,3 Tareq Alrefae,4 Liaohai Leo Chen5 1Department of Chemistry, Kuwait University, Safat, Kuwait; 2King Abdullah International Medical Research Center (KAIMRC), Jeddah, Kingdom of Saudi Arabia; 3Department of Radiology and Nuclear Medicine, University Hospital of Gent (UZG), Gent, Belgium; 4Department of Physics, Kuwait University, Safat, Kuwait; 5Surgical Precision Research Lab. Department of Surgery, University of Illinois at Chicago, IL, USA Abstract: Screening and early diagnosis are the key factors for the reduction of mortality rate and treatment cost of cancer. Therefore, sensitive and selective methods that can reveal the low abundance of cancer biomarkers in a biological sample are always desired. Here, we report the development of a novel electrochemical biosensor for early detection of breast cancer by using bioconjugated self-assembled pH-responsive polymeric micelles. The micelles were loaded with ferrocene molecules as “tracers” to specifically target cell surface-associated epithelial mucin (MUC1), a biomarker for breast and other solid carcinoma. The synthesis of target-specific, ferrocene-loaded polymeric micelles was confirmed, and the resulting sensor was capable of detecting the presence of MUC1 in a sample containing about 10 cells/mL. Such a high sensitivity was achieved by maximizing the loading capacity of ferrocene inside the polymeric micelles. Every single event of binding between the antibody and antigen was represented by the signal of hundreds of thousands of ferrocene molecules that were released from the polymeric micelles. This resulted in a significant increase in the intensity of the ferrocene signal detected by cyclic voltammetry. Keywords: electrochemical immunoassay, polymeric nanoparticles, breast cancer biomarkers, biosensors Mouffouk FAouabdi SAl-Hetlani ESerrai HAlrefae TLeo Chen LDove Medical PressarticleElectrochemical immunoassayPolymeric nanoparticlesBreast cancer biomarkersBiosensors.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 3037-3047 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Electrochemical immunoassay Polymeric nanoparticles Breast cancer biomarkers Biosensors. Medicine (General) R5-920
spellingShingle	Electrochemical immunoassay Polymeric nanoparticles Breast cancer biomarkers Biosensors. Medicine (General) R5-920 Mouffouk F Aouabdi S Al-Hetlani E Serrai H Alrefae T Leo Chen L New generation of electrochemical immunoassay based on polymeric nanoparticles for early detection of breast cancer
description	Fouzi Mouffouk,1 Sihem Aouabdi,2 Entesar Al-Hetlani,1 Hacene Serrai,3 Tareq Alrefae,4 Liaohai Leo Chen5 1Department of Chemistry, Kuwait University, Safat, Kuwait; 2King Abdullah International Medical Research Center (KAIMRC), Jeddah, Kingdom of Saudi Arabia; 3Department of Radiology and Nuclear Medicine, University Hospital of Gent (UZG), Gent, Belgium; 4Department of Physics, Kuwait University, Safat, Kuwait; 5Surgical Precision Research Lab. Department of Surgery, University of Illinois at Chicago, IL, USA Abstract: Screening and early diagnosis are the key factors for the reduction of mortality rate and treatment cost of cancer. Therefore, sensitive and selective methods that can reveal the low abundance of cancer biomarkers in a biological sample are always desired. Here, we report the development of a novel electrochemical biosensor for early detection of breast cancer by using bioconjugated self-assembled pH-responsive polymeric micelles. The micelles were loaded with ferrocene molecules as “tracers” to specifically target cell surface-associated epithelial mucin (MUC1), a biomarker for breast and other solid carcinoma. The synthesis of target-specific, ferrocene-loaded polymeric micelles was confirmed, and the resulting sensor was capable of detecting the presence of MUC1 in a sample containing about 10 cells/mL. Such a high sensitivity was achieved by maximizing the loading capacity of ferrocene inside the polymeric micelles. Every single event of binding between the antibody and antigen was represented by the signal of hundreds of thousands of ferrocene molecules that were released from the polymeric micelles. This resulted in a significant increase in the intensity of the ferrocene signal detected by cyclic voltammetry. Keywords: electrochemical immunoassay, polymeric nanoparticles, breast cancer biomarkers, biosensors
format	article
author	Mouffouk F Aouabdi S Al-Hetlani E Serrai H Alrefae T Leo Chen L
author_facet	Mouffouk F Aouabdi S Al-Hetlani E Serrai H Alrefae T Leo Chen L
author_sort	Mouffouk F
title	New generation of electrochemical immunoassay based on polymeric nanoparticles for early detection of breast cancer
title_short	New generation of electrochemical immunoassay based on polymeric nanoparticles for early detection of breast cancer
title_full	New generation of electrochemical immunoassay based on polymeric nanoparticles for early detection of breast cancer
title_fullStr	New generation of electrochemical immunoassay based on polymeric nanoparticles for early detection of breast cancer
title_full_unstemmed	New generation of electrochemical immunoassay based on polymeric nanoparticles for early detection of breast cancer
title_sort	new generation of electrochemical immunoassay based on polymeric nanoparticles for early detection of breast cancer
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/a9244fdcd23143b2a3d604fba02686a2
work_keys_str_mv	AT mouffoukf newgenerationofelectrochemicalimmunoassaybasedonpolymericnanoparticlesforearlydetectionofbreastcancer AT aouabdis newgenerationofelectrochemicalimmunoassaybasedonpolymericnanoparticlesforearlydetectionofbreastcancer AT alhetlanie newgenerationofelectrochemicalimmunoassaybasedonpolymericnanoparticlesforearlydetectionofbreastcancer AT serraih newgenerationofelectrochemicalimmunoassaybasedonpolymericnanoparticlesforearlydetectionofbreastcancer AT alrefaet newgenerationofelectrochemicalimmunoassaybasedonpolymericnanoparticlesforearlydetectionofbreastcancer AT leochenl newgenerationofelectrochemicalimmunoassaybasedonpolymericnanoparticlesforearlydetectionofbreastcancer
_version_	1718402977228652544

New generation of electrochemical immunoassay based on polymeric nanoparticles for early detection of breast cancer

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