RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis

Abstract The drought-adapted shrub guayule (Parthenium argentatum) produces rubber, a natural product of major commercial importance, and two co-products with potential industrial use: terpene resin and the carbohydrate fructan. The rubber content of guayule plants subjected to water stress is highe...

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Autores principales:	Chen Dong, Grisel Ponciano, Naxin Huo, Yong Gu, Daniel Ilut, Colleen McMahan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a9281b284b224244b7d371f795b2222a
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spelling	oai:doaj.org-article:a9281b284b224244b7d371f795b2222a2021-11-08T10:51:57ZRNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis10.1038/s41598-021-01026-72045-2322https://doaj.org/article/a9281b284b224244b7d371f795b2222a2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01026-7https://doaj.org/toc/2045-2322Abstract The drought-adapted shrub guayule (Parthenium argentatum) produces rubber, a natural product of major commercial importance, and two co-products with potential industrial use: terpene resin and the carbohydrate fructan. The rubber content of guayule plants subjected to water stress is higher compared to that of well-irrigated plants, a fact consistently reported in guayule field evaluations. To better understand how drought influences rubber biosynthesis at the molecular level, a comprehensive transcriptome database was built from drought-stressed guayule stem tissues using de novo RNA-seq and genome-guided assembly, followed by annotation and expression analysis. Despite having higher rubber content, most rubber biosynthesis related genes were down-regulated in drought-stressed guayule, compared to well-irrigated plants, suggesting post-transcriptional effects may regulate drought-induced rubber accumulation. On the other hand, terpene resin biosynthesis genes were unevenly affected by water stress, implying unique environmental influences over transcriptional control of different terpene compounds or classes. Finally, drought induced expression of fructan catabolism genes in guayule and significantly suppressed these fructan biosynthesis genes. It appears then, that in guayule cultivation, irrigation levels might be calibrated in such a regime to enable tunable accumulation of rubber, resin and fructan.Chen DongGrisel PoncianoNaxin HuoYong GuDaniel IlutColleen McMahanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chen Dong Grisel Ponciano Naxin Huo Yong Gu Daniel Ilut Colleen McMahan RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis
description	Abstract The drought-adapted shrub guayule (Parthenium argentatum) produces rubber, a natural product of major commercial importance, and two co-products with potential industrial use: terpene resin and the carbohydrate fructan. The rubber content of guayule plants subjected to water stress is higher compared to that of well-irrigated plants, a fact consistently reported in guayule field evaluations. To better understand how drought influences rubber biosynthesis at the molecular level, a comprehensive transcriptome database was built from drought-stressed guayule stem tissues using de novo RNA-seq and genome-guided assembly, followed by annotation and expression analysis. Despite having higher rubber content, most rubber biosynthesis related genes were down-regulated in drought-stressed guayule, compared to well-irrigated plants, suggesting post-transcriptional effects may regulate drought-induced rubber accumulation. On the other hand, terpene resin biosynthesis genes were unevenly affected by water stress, implying unique environmental influences over transcriptional control of different terpene compounds or classes. Finally, drought induced expression of fructan catabolism genes in guayule and significantly suppressed these fructan biosynthesis genes. It appears then, that in guayule cultivation, irrigation levels might be calibrated in such a regime to enable tunable accumulation of rubber, resin and fructan.
format	article
author	Chen Dong Grisel Ponciano Naxin Huo Yong Gu Daniel Ilut Colleen McMahan
author_facet	Chen Dong Grisel Ponciano Naxin Huo Yong Gu Daniel Ilut Colleen McMahan
author_sort	Chen Dong
title	RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis
title_short	RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis
title_full	RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis
title_fullStr	RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis
title_full_unstemmed	RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis
title_sort	rnaseq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a9281b284b224244b7d371f795b2222a
work_keys_str_mv	AT chendong rnaseqanalysisofdroughtstressedguayulerevealstheroleofgenetranscriptionformodulatingrubberresinandcarbohydratesynthesis AT griselponciano rnaseqanalysisofdroughtstressedguayulerevealstheroleofgenetranscriptionformodulatingrubberresinandcarbohydratesynthesis AT naxinhuo rnaseqanalysisofdroughtstressedguayulerevealstheroleofgenetranscriptionformodulatingrubberresinandcarbohydratesynthesis AT yonggu rnaseqanalysisofdroughtstressedguayulerevealstheroleofgenetranscriptionformodulatingrubberresinandcarbohydratesynthesis AT danielilut rnaseqanalysisofdroughtstressedguayulerevealstheroleofgenetranscriptionformodulatingrubberresinandcarbohydratesynthesis AT colleenmcmahan rnaseqanalysisofdroughtstressedguayulerevealstheroleofgenetranscriptionformodulatingrubberresinandcarbohydratesynthesis
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RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis

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