Metal nanoparticle antibacterial effect оn antibiotic-resistant strains of bacteria

The rapid formation of microbial resistance to modern antibacterial drugs requires to search for new, alternative therapies. It is known that some organisms, such as plants, algae, fungi, are able to convert inorganic metal ions into metal nanoparticles due to the recovery process carried out by pro...

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Autores principales:	E. S. Udegova, K. A. Gildeeva, T. V. Rukosueva, S. Baker
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2021
Materias:	antibiotic resistance polyresistance antibacterial effect metal nanoparticles green synthesis silver copper gold Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/a92a3408c0004aa6a341a576af2cbce7
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spelling	oai:doaj.org-article:a92a3408c0004aa6a341a576af2cbce72021-11-22T07:09:55ZMetal nanoparticle antibacterial effect оn antibiotic-resistant strains of bacteria2220-76192313-739810.15789/2220-7619-MNA-1359https://doaj.org/article/a92a3408c0004aa6a341a576af2cbce72021-09-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1359https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398The rapid formation of microbial resistance to modern antibacterial drugs requires to search for new, alternative therapies. It is known that some organisms, such as plants, algae, fungi, are able to convert inorganic metal ions into metal nanoparticles due to the recovery process carried out by proteins, sugars and metabolites contained in the tissues and cells of these organisms. At the same time, many plants (e.g., plantain, yarrow, wormwood, turmeric long, calendula, marsh bagulnik, etc.) and metals (copper, silver, gold, zinc, etc.) themselves have antibacterial properties, so that metal nanoparticles obtained by biological method, or via “Green” synthesis method, from extracts of such plants can become a current alternative to many modern antibacterial drugs. The antibacterial mechanism of action of nanoparticles depends on the type of microorganisms affected, as well as on the type of nanoparticles, their concentration, size, and how they are obtained. Based on this, the study of the antibacterial effect of nanoparticles is one of the promising directions of solving the problem of microbial antibiotic resistance. There was examined antibacterial effect of metal nanoparticles containing silver, copper and gold obtained by biological method from the salts of AgNO3, CuSO4, H[AuCl4] metals, respectively, and the extract of the plant — turmeric long (lat. Curcuma longa) — related to the following bacteria strain collection: E. coli (ATCC 25922), S. aureus (ATCC 25923), MRSA (ATCC 38591) and polyresistant clinical strains isolated from patients of the Regional clinical hospital (Krasnoyarsk) — К. рneumoniae, strain 104, P. аeruginosa, strain 40, P. аeruginosa, strain 215, А. baumannii, strain 210, А. baumannii, strain 211. Study allowed to identify the minimum suppressive concentration of nanoparticles by the method of serial dilutions (MUK 4.2.1890-04) with azurin dye. It was proved that metal nanoparticles exhibit different antibacterial efficacy depending on the type of nanometals used and bacterial cultures. Copper nanoparticles have the highest antibacterial activity, and gold nanoparticles have the lowest. The most marked antibacterial effect was observed against clinical polyresistant strains. Metal nanoparticles can become an alternative to the currently known antibacterial drugs, but despite the high efficiency of nanoparticles against polyresistant to antibacterial drugs microorganisms in vitro, it is necessary to take into account their possible toxic effect on live tissues, which requires further study in experiments in vivo.E. S. UdegovaK. A. GildeevaT. V. RukosuevaS. BakerSankt-Peterburg : NIIÈM imeni Pasteraarticleantibiotic resistancepolyresistanceantibacterial effectmetal nanoparticlesgreen synthesissilvercoppergoldInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 11, Iss 4, Pp 771-776 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	antibiotic resistance polyresistance antibacterial effect metal nanoparticles green synthesis silver copper gold Infectious and parasitic diseases RC109-216
spellingShingle	antibiotic resistance polyresistance antibacterial effect metal nanoparticles green synthesis silver copper gold Infectious and parasitic diseases RC109-216 E. S. Udegova K. A. Gildeeva T. V. Rukosueva S. Baker Metal nanoparticle antibacterial effect оn antibiotic-resistant strains of bacteria
description	The rapid formation of microbial resistance to modern antibacterial drugs requires to search for new, alternative therapies. It is known that some organisms, such as plants, algae, fungi, are able to convert inorganic metal ions into metal nanoparticles due to the recovery process carried out by proteins, sugars and metabolites contained in the tissues and cells of these organisms. At the same time, many plants (e.g., plantain, yarrow, wormwood, turmeric long, calendula, marsh bagulnik, etc.) and metals (copper, silver, gold, zinc, etc.) themselves have antibacterial properties, so that metal nanoparticles obtained by biological method, or via “Green” synthesis method, from extracts of such plants can become a current alternative to many modern antibacterial drugs. The antibacterial mechanism of action of nanoparticles depends on the type of microorganisms affected, as well as on the type of nanoparticles, their concentration, size, and how they are obtained. Based on this, the study of the antibacterial effect of nanoparticles is one of the promising directions of solving the problem of microbial antibiotic resistance. There was examined antibacterial effect of metal nanoparticles containing silver, copper and gold obtained by biological method from the salts of AgNO3, CuSO4, H[AuCl4] metals, respectively, and the extract of the plant — turmeric long (lat. Curcuma longa) — related to the following bacteria strain collection: E. coli (ATCC 25922), S. aureus (ATCC 25923), MRSA (ATCC 38591) and polyresistant clinical strains isolated from patients of the Regional clinical hospital (Krasnoyarsk) — К. рneumoniae, strain 104, P. аeruginosa, strain 40, P. аeruginosa, strain 215, А. baumannii, strain 210, А. baumannii, strain 211. Study allowed to identify the minimum suppressive concentration of nanoparticles by the method of serial dilutions (MUK 4.2.1890-04) with azurin dye. It was proved that metal nanoparticles exhibit different antibacterial efficacy depending on the type of nanometals used and bacterial cultures. Copper nanoparticles have the highest antibacterial activity, and gold nanoparticles have the lowest. The most marked antibacterial effect was observed against clinical polyresistant strains. Metal nanoparticles can become an alternative to the currently known antibacterial drugs, but despite the high efficiency of nanoparticles against polyresistant to antibacterial drugs microorganisms in vitro, it is necessary to take into account their possible toxic effect on live tissues, which requires further study in experiments in vivo.
format	article
author	E. S. Udegova K. A. Gildeeva T. V. Rukosueva S. Baker
author_facet	E. S. Udegova K. A. Gildeeva T. V. Rukosueva S. Baker
author_sort	E. S. Udegova
title	Metal nanoparticle antibacterial effect оn antibiotic-resistant strains of bacteria
title_short	Metal nanoparticle antibacterial effect оn antibiotic-resistant strains of bacteria
title_full	Metal nanoparticle antibacterial effect оn antibiotic-resistant strains of bacteria
title_fullStr	Metal nanoparticle antibacterial effect оn antibiotic-resistant strains of bacteria
title_full_unstemmed	Metal nanoparticle antibacterial effect оn antibiotic-resistant strains of bacteria
title_sort	metal nanoparticle antibacterial effect оn antibiotic-resistant strains of bacteria
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2021
url	https://doaj.org/article/a92a3408c0004aa6a341a576af2cbce7
work_keys_str_mv	AT esudegova metalnanoparticleantibacterialeffectonantibioticresistantstrainsofbacteria AT kagildeeva metalnanoparticleantibacterialeffectonantibioticresistantstrainsofbacteria AT tvrukosueva metalnanoparticleantibacterialeffectonantibioticresistantstrainsofbacteria AT sbaker metalnanoparticleantibacterialeffectonantibioticresistantstrainsofbacteria
_version_	1718417936019881984

Metal nanoparticle antibacterial effect оn antibiotic-resistant strains of bacteria

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