A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells

A prior live-cell exosome reporter showed dim fluorescence and could not be expressed stably, limiting its usefulness. Here the authors stabilise the reporter to allow long-term tracking of exosomes, and incorporate a second fluorophore to visualise the entire exosome lifecycle.

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Autores principales:	Bong Hwan Sung, Ariana von Lersner, Jorge Guerrero, Evan S. Krystofiak, David Inman, Roxanne Pelletier, Andries Zijlstra, Suzanne M. Ponik, Alissa M. Weaver
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a92f2f1ca0644dc2b5577af154c973ca
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spelling	oai:doaj.org-article:a92f2f1ca0644dc2b5577af154c973ca2021-12-02T16:50:00ZA live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells10.1038/s41467-020-15747-22041-1723https://doaj.org/article/a92f2f1ca0644dc2b5577af154c973ca2020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15747-2https://doaj.org/toc/2041-1723A prior live-cell exosome reporter showed dim fluorescence and could not be expressed stably, limiting its usefulness. Here the authors stabilise the reporter to allow long-term tracking of exosomes, and incorporate a second fluorophore to visualise the entire exosome lifecycle.Bong Hwan SungAriana von LersnerJorge GuerreroEvan S. KrystofiakDavid InmanRoxanne PelletierAndries ZijlstraSuzanne M. PonikAlissa M. WeaverNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Bong Hwan Sung Ariana von Lersner Jorge Guerrero Evan S. Krystofiak David Inman Roxanne Pelletier Andries Zijlstra Suzanne M. Ponik Alissa M. Weaver A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells
description	A prior live-cell exosome reporter showed dim fluorescence and could not be expressed stably, limiting its usefulness. Here the authors stabilise the reporter to allow long-term tracking of exosomes, and incorporate a second fluorophore to visualise the entire exosome lifecycle.
format	article
author	Bong Hwan Sung Ariana von Lersner Jorge Guerrero Evan S. Krystofiak David Inman Roxanne Pelletier Andries Zijlstra Suzanne M. Ponik Alissa M. Weaver
author_facet	Bong Hwan Sung Ariana von Lersner Jorge Guerrero Evan S. Krystofiak David Inman Roxanne Pelletier Andries Zijlstra Suzanne M. Ponik Alissa M. Weaver
author_sort	Bong Hwan Sung
title	A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells
title_short	A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells
title_full	A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells
title_fullStr	A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells
title_full_unstemmed	A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells
title_sort	live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/a92f2f1ca0644dc2b5577af154c973ca
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A live cell reporter of exosome secretion and uptake reveals pathfinding behavior of migrating cells

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