In vitro and in vivo germ line potential of stem cells derived from newborn mouse skin.

We previously reported that fetal porcine skin-derived stem cells were capable of differentiation into oocyte-like cells (OLCs). Here we report that newborn mice skin-derived stem cells are also capable of differentiating into early OLCs. Using stem cells from mice that are transgenic for Oct4 germl...

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Autores principales:	Paul W Dyce, Jinghe Liu, Chandrakant Tayade, Gerald M Kidder, Dean H Betts, Julang Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a9327553b9aa401cbbdd084adddccc3a
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spelling	oai:doaj.org-article:a9327553b9aa401cbbdd084adddccc3a2021-11-18T06:53:23ZIn vitro and in vivo germ line potential of stem cells derived from newborn mouse skin.1932-620310.1371/journal.pone.0020339https://doaj.org/article/a9327553b9aa401cbbdd084adddccc3a2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21629667/?tool=EBIhttps://doaj.org/toc/1932-6203We previously reported that fetal porcine skin-derived stem cells were capable of differentiation into oocyte-like cells (OLCs). Here we report that newborn mice skin-derived stem cells are also capable of differentiating into early OLCs. Using stem cells from mice that are transgenic for Oct4 germline distal enhancer-GFP, germ cells resulting from their differentiation are expected to be GFP(+). After differentiation, some GFP(+) OLCs reached 40-45 µM and expressed oocyte markers. Flow cytometric analysis revealed that ∼ 0.3% of the freshly isolated skin cells were GFP(+). The GFP-positive cells increased to ∼ 7% after differentiation, suggesting that the GFP(+) cells could be of in vivo origin, but are more likely induced upon being cultured in vitro. To study the in vivo germ cell potential of skin-derived cells, they were aggregated with newborn ovarian cells, and transplanted under the kidney capsule of ovariectomized mice. GFP(+) oocytes were identified within a subpopulation of follicles in the resulting growth. Our finding that early oocytes can be differentiated from mice skin-derived cells in defined medium may offer a new in vitro model to study germ cell formation and oogenesis.Paul W DyceJinghe LiuChandrakant TayadeGerald M KidderDean H BettsJulang LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e20339 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Paul W Dyce Jinghe Liu Chandrakant Tayade Gerald M Kidder Dean H Betts Julang Li In vitro and in vivo germ line potential of stem cells derived from newborn mouse skin.
description	We previously reported that fetal porcine skin-derived stem cells were capable of differentiation into oocyte-like cells (OLCs). Here we report that newborn mice skin-derived stem cells are also capable of differentiating into early OLCs. Using stem cells from mice that are transgenic for Oct4 germline distal enhancer-GFP, germ cells resulting from their differentiation are expected to be GFP(+). After differentiation, some GFP(+) OLCs reached 40-45 µM and expressed oocyte markers. Flow cytometric analysis revealed that ∼ 0.3% of the freshly isolated skin cells were GFP(+). The GFP-positive cells increased to ∼ 7% after differentiation, suggesting that the GFP(+) cells could be of in vivo origin, but are more likely induced upon being cultured in vitro. To study the in vivo germ cell potential of skin-derived cells, they were aggregated with newborn ovarian cells, and transplanted under the kidney capsule of ovariectomized mice. GFP(+) oocytes were identified within a subpopulation of follicles in the resulting growth. Our finding that early oocytes can be differentiated from mice skin-derived cells in defined medium may offer a new in vitro model to study germ cell formation and oogenesis.
format	article
author	Paul W Dyce Jinghe Liu Chandrakant Tayade Gerald M Kidder Dean H Betts Julang Li
author_facet	Paul W Dyce Jinghe Liu Chandrakant Tayade Gerald M Kidder Dean H Betts Julang Li
author_sort	Paul W Dyce
title	In vitro and in vivo germ line potential of stem cells derived from newborn mouse skin.
title_short	In vitro and in vivo germ line potential of stem cells derived from newborn mouse skin.
title_full	In vitro and in vivo germ line potential of stem cells derived from newborn mouse skin.
title_fullStr	In vitro and in vivo germ line potential of stem cells derived from newborn mouse skin.
title_full_unstemmed	In vitro and in vivo germ line potential of stem cells derived from newborn mouse skin.
title_sort	in vitro and in vivo germ line potential of stem cells derived from newborn mouse skin.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a9327553b9aa401cbbdd084adddccc3a
work_keys_str_mv	AT paulwdyce invitroandinvivogermlinepotentialofstemcellsderivedfromnewbornmouseskin AT jingheliu invitroandinvivogermlinepotentialofstemcellsderivedfromnewbornmouseskin AT chandrakanttayade invitroandinvivogermlinepotentialofstemcellsderivedfromnewbornmouseskin AT geraldmkidder invitroandinvivogermlinepotentialofstemcellsderivedfromnewbornmouseskin AT deanhbetts invitroandinvivogermlinepotentialofstemcellsderivedfromnewbornmouseskin AT julangli invitroandinvivogermlinepotentialofstemcellsderivedfromnewbornmouseskin
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In vitro and in vivo germ line potential of stem cells derived from newborn mouse skin.

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