Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei

The dorsal raphe nucleus (DR) and median raphe nucleus (MR) contain populations of glutamatergic and GABAergic neurons that regulate diverse behavioral functions. However, their whole-brain input-output circuits remain incompletely elucidated. We used viral tracing combined with fluorescence micro-o...

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Autores principales:	Zhengchao Xu, Zhao Feng, Mengting Zhao, Qingtao Sun, Lei Deng, Xueyan Jia, Tao Jiang, Pan Luo, Wu Chen, Ayizuohere Tudi, Jing Yuan, Xiangning Li, Hui Gong, Qingming Luo, Anan Li
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	glutamatergic neuron GABAergic neuron raphe nucleus Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a93515dcd0604a538c51411e832e0ed3
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spelling	oai:doaj.org-article:a93515dcd0604a538c51411e832e0ed32021-11-26T14:23:07ZWhole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei10.7554/eLife.655022050-084Xe65502https://doaj.org/article/a93515dcd0604a538c51411e832e0ed32021-11-01T00:00:00Zhttps://elifesciences.org/articles/65502https://doaj.org/toc/2050-084XThe dorsal raphe nucleus (DR) and median raphe nucleus (MR) contain populations of glutamatergic and GABAergic neurons that regulate diverse behavioral functions. However, their whole-brain input-output circuits remain incompletely elucidated. We used viral tracing combined with fluorescence micro-optical sectioning tomography to generate a comprehensive whole-brain atlas of inputs and outputs of glutamatergic and GABAergic neurons in the DR and MR. We found that these neurons received inputs from similar upstream brain regions. The glutamatergic and GABAergic neurons in the same raphe nucleus had divergent projection patterns with differences in critical brain regions. Specifically, MR glutamatergic neurons projected to the lateral habenula through multiple pathways. Correlation and cluster analysis revealed that glutamatergic and GABAergic neurons in the same raphe nucleus received heterogeneous inputs and sent different collateral projections. This connectivity atlas further elucidates the anatomical architecture of the raphe nuclei, which could facilitate better understanding of their behavioral functions.Zhengchao XuZhao FengMengting ZhaoQingtao SunLei DengXueyan JiaTao JiangPan LuoWu ChenAyizuohere TudiJing YuanXiangning LiHui GongQingming LuoAnan LieLife Sciences Publications Ltdarticleglutamatergic neuronGABAergic neuronraphe nucleusMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glutamatergic neuron GABAergic neuron raphe nucleus Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	glutamatergic neuron GABAergic neuron raphe nucleus Medicine R Science Q Biology (General) QH301-705.5 Zhengchao Xu Zhao Feng Mengting Zhao Qingtao Sun Lei Deng Xueyan Jia Tao Jiang Pan Luo Wu Chen Ayizuohere Tudi Jing Yuan Xiangning Li Hui Gong Qingming Luo Anan Li Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei
description	The dorsal raphe nucleus (DR) and median raphe nucleus (MR) contain populations of glutamatergic and GABAergic neurons that regulate diverse behavioral functions. However, their whole-brain input-output circuits remain incompletely elucidated. We used viral tracing combined with fluorescence micro-optical sectioning tomography to generate a comprehensive whole-brain atlas of inputs and outputs of glutamatergic and GABAergic neurons in the DR and MR. We found that these neurons received inputs from similar upstream brain regions. The glutamatergic and GABAergic neurons in the same raphe nucleus had divergent projection patterns with differences in critical brain regions. Specifically, MR glutamatergic neurons projected to the lateral habenula through multiple pathways. Correlation and cluster analysis revealed that glutamatergic and GABAergic neurons in the same raphe nucleus received heterogeneous inputs and sent different collateral projections. This connectivity atlas further elucidates the anatomical architecture of the raphe nuclei, which could facilitate better understanding of their behavioral functions.
format	article
author	Zhengchao Xu Zhao Feng Mengting Zhao Qingtao Sun Lei Deng Xueyan Jia Tao Jiang Pan Luo Wu Chen Ayizuohere Tudi Jing Yuan Xiangning Li Hui Gong Qingming Luo Anan Li
author_facet	Zhengchao Xu Zhao Feng Mengting Zhao Qingtao Sun Lei Deng Xueyan Jia Tao Jiang Pan Luo Wu Chen Ayizuohere Tudi Jing Yuan Xiangning Li Hui Gong Qingming Luo Anan Li
author_sort	Zhengchao Xu
title	Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei
title_short	Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei
title_full	Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei
title_fullStr	Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei
title_full_unstemmed	Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei
title_sort	whole-brain connectivity atlas of glutamatergic and gabaergic neurons in the mouse dorsal and median raphe nuclei
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/a93515dcd0604a538c51411e832e0ed3
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Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei

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