Inheritance of DNA transferred from American trypanosomes to human hosts.

Interspecies DNA transfer is a major biological process leading to the accumulation of mutations inherited by sexual reproduction among eukaryotes. Lateral DNA transfer events and their inheritance has been challenging to document. In this study we modified a thermal asymmetric interlaced PCR by usi...

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Autores principales:	Mariana M Hecht, Nadjar Nitz, Perla F Araujo, Alessandro O Sousa, Ana de Cássia Rosa, Dawidson A Gomes, Eduardo Leonardecz, Antonio R L Teixeira
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a93a6633e50b4153a0c4da03b6b2b0e7
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spelling	oai:doaj.org-article:a93a6633e50b4153a0c4da03b6b2b0e72021-11-25T06:25:54ZInheritance of DNA transferred from American trypanosomes to human hosts.1932-620310.1371/journal.pone.0009181https://doaj.org/article/a93a6633e50b4153a0c4da03b6b2b0e72010-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20169193/?tool=EBIhttps://doaj.org/toc/1932-6203Interspecies DNA transfer is a major biological process leading to the accumulation of mutations inherited by sexual reproduction among eukaryotes. Lateral DNA transfer events and their inheritance has been challenging to document. In this study we modified a thermal asymmetric interlaced PCR by using additional targeted primers, along with Southern blots, fluorescence techniques, and bioinformatics, to identify lateral DNA transfer events from parasite to host. Instances of naturally occurring human infections by Trypanosoma cruzi are documented, where mitochondrial minicircles integrated mainly into retrotransposable LINE-1 of various chromosomes. The founders of five families show minicircle integrations that were transferred vertically to their progeny. Microhomology end-joining of 6 to 22 AC-rich nucleotide repeats in the minicircles and host DNA mediates foreign DNA integration. Heterogeneous minicircle sequences were distributed randomly among families, with diversity increasing due to subsequent rearrangement of inserted fragments. Mosaic recombination and hitchhiking on retrotransposition events to different loci were more prevalent in germ line as compared to somatic cells. Potential new genes, pseudogenes, and knockouts were identified. A pathway of minicircle integration and maintenance in the host genome is suggested. Thus, infection by T. cruzi has the unexpected consequence of increasing human genetic diversity, and Chagas disease may be a fortuitous share of negative selection. This demonstration of contemporary transfer of eukaryotic DNA to the human genome and its subsequent inheritance by descendants introduces a significant change in the scientific concept of evolutionary biology and medicine.Mariana M HechtNadjar NitzPerla F AraujoAlessandro O SousaAna de Cássia RosaDawidson A GomesEduardo LeonardeczAntonio R L TeixeiraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 2, p e9181 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariana M Hecht Nadjar Nitz Perla F Araujo Alessandro O Sousa Ana de Cássia Rosa Dawidson A Gomes Eduardo Leonardecz Antonio R L Teixeira Inheritance of DNA transferred from American trypanosomes to human hosts.
description	Interspecies DNA transfer is a major biological process leading to the accumulation of mutations inherited by sexual reproduction among eukaryotes. Lateral DNA transfer events and their inheritance has been challenging to document. In this study we modified a thermal asymmetric interlaced PCR by using additional targeted primers, along with Southern blots, fluorescence techniques, and bioinformatics, to identify lateral DNA transfer events from parasite to host. Instances of naturally occurring human infections by Trypanosoma cruzi are documented, where mitochondrial minicircles integrated mainly into retrotransposable LINE-1 of various chromosomes. The founders of five families show minicircle integrations that were transferred vertically to their progeny. Microhomology end-joining of 6 to 22 AC-rich nucleotide repeats in the minicircles and host DNA mediates foreign DNA integration. Heterogeneous minicircle sequences were distributed randomly among families, with diversity increasing due to subsequent rearrangement of inserted fragments. Mosaic recombination and hitchhiking on retrotransposition events to different loci were more prevalent in germ line as compared to somatic cells. Potential new genes, pseudogenes, and knockouts were identified. A pathway of minicircle integration and maintenance in the host genome is suggested. Thus, infection by T. cruzi has the unexpected consequence of increasing human genetic diversity, and Chagas disease may be a fortuitous share of negative selection. This demonstration of contemporary transfer of eukaryotic DNA to the human genome and its subsequent inheritance by descendants introduces a significant change in the scientific concept of evolutionary biology and medicine.
format	article
author	Mariana M Hecht Nadjar Nitz Perla F Araujo Alessandro O Sousa Ana de Cássia Rosa Dawidson A Gomes Eduardo Leonardecz Antonio R L Teixeira
author_facet	Mariana M Hecht Nadjar Nitz Perla F Araujo Alessandro O Sousa Ana de Cássia Rosa Dawidson A Gomes Eduardo Leonardecz Antonio R L Teixeira
author_sort	Mariana M Hecht
title	Inheritance of DNA transferred from American trypanosomes to human hosts.
title_short	Inheritance of DNA transferred from American trypanosomes to human hosts.
title_full	Inheritance of DNA transferred from American trypanosomes to human hosts.
title_fullStr	Inheritance of DNA transferred from American trypanosomes to human hosts.
title_full_unstemmed	Inheritance of DNA transferred from American trypanosomes to human hosts.
title_sort	inheritance of dna transferred from american trypanosomes to human hosts.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/a93a6633e50b4153a0c4da03b6b2b0e7
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Inheritance of DNA transferred from American trypanosomes to human hosts.

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