Serum- and glucocorticoid-regulated kinase 3 promotes proliferation and inhibits apoptosis of ER+ breast cancer cells

Objective To investigate the effects of serum- and glucocorticoid-regulated kinase 3 (SGK3) on the proliferation and apoptosis of ER positive breast cancer cell lines MCF-7 and T47D. Methods Gene expression profile interactive analysis (GEPIA) was used to analyze SGK3 expression levels in BRCA (brea...

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Autores principales:	LIU Xu, LI Xiaoli, ZHOU Duanfang, CHEN Bo, SONG Y, HE Qichen
Formato:	article
Lenguaje:	ZH
Publicado:	Editorial Office of Journal of Third Military Medical University 2021
Materias:	serum- and glucocorticoid-regulated kinase 3 breast cancer proliferation apoptosis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a93de5390af641ec956d19c8c9939520
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spelling	oai:doaj.org-article:a93de5390af641ec956d19c8c99395202021-11-28T10:42:10ZSerum- and glucocorticoid-regulated kinase 3 promotes proliferation and inhibits apoptosis of ER+ breast cancer cells10.16016/j.1000-5404.2021051551000-5404https://doaj.org/article/a93de5390af641ec956d19c8c99395202021-11-01T00:00:00Zhttp://aammt.tmmu.edu.cn/Upload/rhtml/202105155.htmhttps://doaj.org/toc/1000-5404Objective To investigate the effects of serum- and glucocorticoid-regulated kinase 3 (SGK3) on the proliferation and apoptosis of ER positive breast cancer cell lines MCF-7 and T47D. Methods Gene expression profile interactive analysis (GEPIA) was used to analyze SGK3 expression levels in BRCA (breast cancer tissue) and normal tissue in the cancer public database, the Cancer Genome Atlas (TCGA) database, and survival analysis was performed based on SGK3 levels in the BRCA patients in the TCGA database. After inducement of SGK3 overexpression in SGK3-inducible breast cancer cell lines MCF-7-Tet-On-SGK3 and T47D-Tet-On-SGK3, MTT assay was used to detect the effect of SGK3 overexpression on cell proliferation. Flow cytometry and Annexin V-FITC apoptosis detection kit were employed to detect the effect of SGK3 overexpression on cell apoptosis. Western blotting was applied to measure the changes of apoptosis-related protein molecules. After SGK3 was knocked down with SGK3 specific small interference RNA, MTT assay was adopted to detect the effect of SGK3 knockdown on the proliferation of MCF-7 cells, flow cytometry for cell apoptosis, and Western blotting for the molecular changes of apoptosis-related proteins. Results The TCGA database had a total of 1 084 BRCA samples and 492 breast control samples. Compared with the control breast tissue, the expression of SGK3 was significantly higher in BRCA. The GEPIA survival curve analysis showed that the expression level of SGK3 was negatively correlated with the overall survival (OS) and disease-free survival (DFS) of BRCA patients. In MCF-7-Tet-On-SGK3 and T47D-Tet-On-SGK3 cells, inducing SGK3 expression promoted cell proliferation and reduced the apoptosis. At molecular level, SGK3 overexpression down-regulated the expression of apoptotic proteins Bax and cleaved-PARP, and up-regulated the level of anti-apoptotic protein Bcl-2. SGK3 knockdown in MCF-7 and T47D cells inhibited cell proliferation and increased cell apoptosis. At the molecular level, knocking SGK3 down increased Bax protein level, reduced Bcl-2 level and up-regulated cleaved-PARP. Conclusion In ER+ breast cancer cells, SGK3 promotes cell proliferation and reduces apoptosis. Its high expression may be associated with poor prognosis in breast cancer patients.LIU XuLI XiaoliZHOU DuanfangCHEN BoSONG YHE QichenEditorial Office of Journal of Third Military Medical Universityarticleserum- and glucocorticoid-regulated kinase 3breast cancerproliferationapoptosisMedicine (General)R5-920ZHDi-san junyi daxue xuebao, Vol 43, Iss 22, Pp 2414-2422 (2021)
institution	DOAJ
collection	DOAJ
language	ZH
topic	serum- and glucocorticoid-regulated kinase 3 breast cancer proliferation apoptosis Medicine (General) R5-920
spellingShingle	serum- and glucocorticoid-regulated kinase 3 breast cancer proliferation apoptosis Medicine (General) R5-920 LIU Xu LI Xiaoli ZHOU Duanfang CHEN Bo SONG Y HE Qichen Serum- and glucocorticoid-regulated kinase 3 promotes proliferation and inhibits apoptosis of ER+ breast cancer cells
description	Objective To investigate the effects of serum- and glucocorticoid-regulated kinase 3 (SGK3) on the proliferation and apoptosis of ER positive breast cancer cell lines MCF-7 and T47D. Methods Gene expression profile interactive analysis (GEPIA) was used to analyze SGK3 expression levels in BRCA (breast cancer tissue) and normal tissue in the cancer public database, the Cancer Genome Atlas (TCGA) database, and survival analysis was performed based on SGK3 levels in the BRCA patients in the TCGA database. After inducement of SGK3 overexpression in SGK3-inducible breast cancer cell lines MCF-7-Tet-On-SGK3 and T47D-Tet-On-SGK3, MTT assay was used to detect the effect of SGK3 overexpression on cell proliferation. Flow cytometry and Annexin V-FITC apoptosis detection kit were employed to detect the effect of SGK3 overexpression on cell apoptosis. Western blotting was applied to measure the changes of apoptosis-related protein molecules. After SGK3 was knocked down with SGK3 specific small interference RNA, MTT assay was adopted to detect the effect of SGK3 knockdown on the proliferation of MCF-7 cells, flow cytometry for cell apoptosis, and Western blotting for the molecular changes of apoptosis-related proteins. Results The TCGA database had a total of 1 084 BRCA samples and 492 breast control samples. Compared with the control breast tissue, the expression of SGK3 was significantly higher in BRCA. The GEPIA survival curve analysis showed that the expression level of SGK3 was negatively correlated with the overall survival (OS) and disease-free survival (DFS) of BRCA patients. In MCF-7-Tet-On-SGK3 and T47D-Tet-On-SGK3 cells, inducing SGK3 expression promoted cell proliferation and reduced the apoptosis. At molecular level, SGK3 overexpression down-regulated the expression of apoptotic proteins Bax and cleaved-PARP, and up-regulated the level of anti-apoptotic protein Bcl-2. SGK3 knockdown in MCF-7 and T47D cells inhibited cell proliferation and increased cell apoptosis. At the molecular level, knocking SGK3 down increased Bax protein level, reduced Bcl-2 level and up-regulated cleaved-PARP. Conclusion In ER+ breast cancer cells, SGK3 promotes cell proliferation and reduces apoptosis. Its high expression may be associated with poor prognosis in breast cancer patients.
format	article
author	LIU Xu LI Xiaoli ZHOU Duanfang CHEN Bo SONG Y HE Qichen
author_facet	LIU Xu LI Xiaoli ZHOU Duanfang CHEN Bo SONG Y HE Qichen
author_sort	LIU Xu
title	Serum- and glucocorticoid-regulated kinase 3 promotes proliferation and inhibits apoptosis of ER+ breast cancer cells
title_short	Serum- and glucocorticoid-regulated kinase 3 promotes proliferation and inhibits apoptosis of ER+ breast cancer cells
title_full	Serum- and glucocorticoid-regulated kinase 3 promotes proliferation and inhibits apoptosis of ER+ breast cancer cells
title_fullStr	Serum- and glucocorticoid-regulated kinase 3 promotes proliferation and inhibits apoptosis of ER+ breast cancer cells
title_full_unstemmed	Serum- and glucocorticoid-regulated kinase 3 promotes proliferation and inhibits apoptosis of ER+ breast cancer cells
title_sort	serum- and glucocorticoid-regulated kinase 3 promotes proliferation and inhibits apoptosis of er+ breast cancer cells
publisher	Editorial Office of Journal of Third Military Medical University
publishDate	2021
url	https://doaj.org/article/a93de5390af641ec956d19c8c9939520
work_keys_str_mv	AT liuxu serumandglucocorticoidregulatedkinase3promotesproliferationandinhibitsapoptosisoferbreastcancercells AT lixiaoli serumandglucocorticoidregulatedkinase3promotesproliferationandinhibitsapoptosisoferbreastcancercells AT zhouduanfang serumandglucocorticoidregulatedkinase3promotesproliferationandinhibitsapoptosisoferbreastcancercells AT chenbo serumandglucocorticoidregulatedkinase3promotesproliferationandinhibitsapoptosisoferbreastcancercells AT songy serumandglucocorticoidregulatedkinase3promotesproliferationandinhibitsapoptosisoferbreastcancercells AT heqichen serumandglucocorticoidregulatedkinase3promotesproliferationandinhibitsapoptosisoferbreastcancercells
_version_	1718408277056815104

Serum- and glucocorticoid-regulated kinase 3 promotes proliferation and inhibits apoptosis of ER+ breast cancer cells

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