Development of microsatellite markers for the house cricket, Acheta domesticus (Orthoptera: Gryllidae)

Abstract. Gupta YM, Tanasarnpaiboon S, Buddhachat K, Peyachoknagul S, Inthim P, Homchan S. 2020. Development of microsatellite markers for the house cricket, Acheta domesticus (Orthoptera: Gryllidae). Biodiversitas 21: 4094-4099. The house cricket, Acheta domesticus, is one of the species of cricket...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yash Munnalal Gupta, SUPATCHAREE TANASARNPAIBOON, KITTISAK BUDDHACHAT, SURIN PEYACHOKNAGUL, PHATTHARAPORN INTHIM, SOMJIT HOMCHAN
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	cross-amplification, microsatellite markers, population genetics, simple sequence repeats Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a9439572113048b29848a33deb1d4813
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a9439572113048b29848a33deb1d4813
record_format	dspace
spelling	oai:doaj.org-article:a9439572113048b29848a33deb1d48132021-11-22T00:41:43ZDevelopment of microsatellite markers for the house cricket, Acheta domesticus (Orthoptera: Gryllidae)1412-033X2085-472210.13057/biodiv/d210921https://doaj.org/article/a9439572113048b29848a33deb1d48132020-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6435https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Gupta YM, Tanasarnpaiboon S, Buddhachat K, Peyachoknagul S, Inthim P, Homchan S. 2020. Development of microsatellite markers for the house cricket, Acheta domesticus (Orthoptera: Gryllidae). Biodiversitas 21: 4094-4099. The house cricket, Acheta domesticus, is one of the species of crickets commonly found in Thailand. Insect breeders in Thailand prefer to breed house cricket as food due to its better taste and popularity among local people. Moreover, largescale breeding industries also breed house cricket to produce cricket-based edible products. Insect breeding industry is growing rapidly and requires primary precaution for sustainable production. To facilitate breeding system to maintain genetic variation in the captive population, we have sequenced the whole genome of A. domesticus to search for simple sequence repeats (SSRs) in order to develop polymorphic microsatellite markers for preliminary population genetic analysis. A total of 112,157 SSRs with primer pairs were identified in our analysis. Of these, 91 were randomly selected to check for amplification of microsatellite polymorphisms. From these, nine microsatellites were used to check genetic variation in forty-five individuals of A. domesticus from the Phitsanulok population (Thailand). These microsatellite markers also showed cross-amplification with other three species of edible crickets, specifically Gryllus bimaculatus, Gryllus testaceus, and Brachytrupes portentosus. The microsatellite markers presented herein will facilitate future population genetic analysis of A. domesticus populations. Moreover, the transferability of these makers would also enable researchers to conduct genetic studies for other closely related species.Yash Munnalal GuptaSUPATCHAREE TANASARNPAIBOONKITTISAK BUDDHACHATSURIN PEYACHOKNAGULPHATTHARAPORN INTHIMSOMJIT HOMCHANMBI & UNS Soloarticlecross-amplification, microsatellite markers, population genetics, simple sequence repeatsBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cross-amplification, microsatellite markers, population genetics, simple sequence repeats Biology (General) QH301-705.5
spellingShingle	cross-amplification, microsatellite markers, population genetics, simple sequence repeats Biology (General) QH301-705.5 Yash Munnalal Gupta SUPATCHAREE TANASARNPAIBOON KITTISAK BUDDHACHAT SURIN PEYACHOKNAGUL PHATTHARAPORN INTHIM SOMJIT HOMCHAN Development of microsatellite markers for the house cricket, Acheta domesticus (Orthoptera: Gryllidae)
description	Abstract. Gupta YM, Tanasarnpaiboon S, Buddhachat K, Peyachoknagul S, Inthim P, Homchan S. 2020. Development of microsatellite markers for the house cricket, Acheta domesticus (Orthoptera: Gryllidae). Biodiversitas 21: 4094-4099. The house cricket, Acheta domesticus, is one of the species of crickets commonly found in Thailand. Insect breeders in Thailand prefer to breed house cricket as food due to its better taste and popularity among local people. Moreover, largescale breeding industries also breed house cricket to produce cricket-based edible products. Insect breeding industry is growing rapidly and requires primary precaution for sustainable production. To facilitate breeding system to maintain genetic variation in the captive population, we have sequenced the whole genome of A. domesticus to search for simple sequence repeats (SSRs) in order to develop polymorphic microsatellite markers for preliminary population genetic analysis. A total of 112,157 SSRs with primer pairs were identified in our analysis. Of these, 91 were randomly selected to check for amplification of microsatellite polymorphisms. From these, nine microsatellites were used to check genetic variation in forty-five individuals of A. domesticus from the Phitsanulok population (Thailand). These microsatellite markers also showed cross-amplification with other three species of edible crickets, specifically Gryllus bimaculatus, Gryllus testaceus, and Brachytrupes portentosus. The microsatellite markers presented herein will facilitate future population genetic analysis of A. domesticus populations. Moreover, the transferability of these makers would also enable researchers to conduct genetic studies for other closely related species.
format	article
author	Yash Munnalal Gupta SUPATCHAREE TANASARNPAIBOON KITTISAK BUDDHACHAT SURIN PEYACHOKNAGUL PHATTHARAPORN INTHIM SOMJIT HOMCHAN
author_facet	Yash Munnalal Gupta SUPATCHAREE TANASARNPAIBOON KITTISAK BUDDHACHAT SURIN PEYACHOKNAGUL PHATTHARAPORN INTHIM SOMJIT HOMCHAN
author_sort	Yash Munnalal Gupta
title	Development of microsatellite markers for the house cricket, Acheta domesticus (Orthoptera: Gryllidae)
title_short	Development of microsatellite markers for the house cricket, Acheta domesticus (Orthoptera: Gryllidae)
title_full	Development of microsatellite markers for the house cricket, Acheta domesticus (Orthoptera: Gryllidae)
title_fullStr	Development of microsatellite markers for the house cricket, Acheta domesticus (Orthoptera: Gryllidae)
title_full_unstemmed	Development of microsatellite markers for the house cricket, Acheta domesticus (Orthoptera: Gryllidae)
title_sort	development of microsatellite markers for the house cricket, acheta domesticus (orthoptera: gryllidae)
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/a9439572113048b29848a33deb1d4813
work_keys_str_mv	AT yashmunnalalgupta developmentofmicrosatellitemarkersforthehousecricketachetadomesticusorthopteragryllidae AT supatchareetanasarnpaiboon developmentofmicrosatellitemarkersforthehousecricketachetadomesticusorthopteragryllidae AT kittisakbuddhachat developmentofmicrosatellitemarkersforthehousecricketachetadomesticusorthopteragryllidae AT surinpeyachoknagul developmentofmicrosatellitemarkersforthehousecricketachetadomesticusorthopteragryllidae AT phattharaporninthim developmentofmicrosatellitemarkersforthehousecricketachetadomesticusorthopteragryllidae AT somjithomchan developmentofmicrosatellitemarkersforthehousecricketachetadomesticusorthopteragryllidae
_version_	1718418542650458112

Development of microsatellite markers for the house cricket, Acheta domesticus (Orthoptera: Gryllidae)

Ejemplares similares