Up-regulation of microRNA-203 in influenza A virus infection inhibits viral replication by targeting DR1

Abstract MicroRNAs (miRNAs) are small noncoding RNA molecules that play important roles in various biological processes. Much evidence shows that miRNAs are closely associated with numerous virus infections; however, involvement of cellular miRNAs in influenza A virus (IAV) infection is unclear. Her...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sen Zhang, Jing Li, Junfeng Li, Yinhui Yang, Xiaoping Kang, Yuchang Li, Xiaoyan Wu, Qingyu Zhu, Yusen Zhou, Yi Hu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a944f0ed5d644c42a79792fc2b06c097
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a944f0ed5d644c42a79792fc2b06c097
record_format	dspace
spelling	oai:doaj.org-article:a944f0ed5d644c42a79792fc2b06c0972021-12-02T16:08:01ZUp-regulation of microRNA-203 in influenza A virus infection inhibits viral replication by targeting DR110.1038/s41598-018-25073-92045-2322https://doaj.org/article/a944f0ed5d644c42a79792fc2b06c0972018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25073-9https://doaj.org/toc/2045-2322Abstract MicroRNAs (miRNAs) are small noncoding RNA molecules that play important roles in various biological processes. Much evidence shows that miRNAs are closely associated with numerous virus infections; however, involvement of cellular miRNAs in influenza A virus (IAV) infection is unclear. Here, we found that expression of miR-203 was up-regulated markedly via two different mechanisms during IAV infection. First, we examined the effects of type I interferon induced by IAV on direct activation of miR-203 expression. Next, we showed that DNA demethylation within the miR-203 promoter region in A549 cells induced its up-regulation, and that expression of DNA methyltransferase 1 was down-regulated following H5N1 virus infection. Ectopic expression of miR-203 in turn inhibited H5N1 virus replication by targeting down-regulator of transcription 1 (DR1), which was identified as a novel target of miR-203. Silencing DR1 in miR-203 knockout cells using a specific siRNA inhibited replication of the H5N1 virus, an effect similar to that of miR-203. In summary, the data show that host cell expression of miR-203 is up-regulated upon IAV infection, which increases antiviral responses by suppressing a novel target gene, DR1. Thus, we have identified a novel mechanism underlying the relationship between miR-203 and IAV infection.Sen ZhangJing LiJunfeng LiYinhui YangXiaoping KangYuchang LiXiaoyan WuQingyu ZhuYusen ZhouYi HuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sen Zhang Jing Li Junfeng Li Yinhui Yang Xiaoping Kang Yuchang Li Xiaoyan Wu Qingyu Zhu Yusen Zhou Yi Hu Up-regulation of microRNA-203 in influenza A virus infection inhibits viral replication by targeting DR1
description	Abstract MicroRNAs (miRNAs) are small noncoding RNA molecules that play important roles in various biological processes. Much evidence shows that miRNAs are closely associated with numerous virus infections; however, involvement of cellular miRNAs in influenza A virus (IAV) infection is unclear. Here, we found that expression of miR-203 was up-regulated markedly via two different mechanisms during IAV infection. First, we examined the effects of type I interferon induced by IAV on direct activation of miR-203 expression. Next, we showed that DNA demethylation within the miR-203 promoter region in A549 cells induced its up-regulation, and that expression of DNA methyltransferase 1 was down-regulated following H5N1 virus infection. Ectopic expression of miR-203 in turn inhibited H5N1 virus replication by targeting down-regulator of transcription 1 (DR1), which was identified as a novel target of miR-203. Silencing DR1 in miR-203 knockout cells using a specific siRNA inhibited replication of the H5N1 virus, an effect similar to that of miR-203. In summary, the data show that host cell expression of miR-203 is up-regulated upon IAV infection, which increases antiviral responses by suppressing a novel target gene, DR1. Thus, we have identified a novel mechanism underlying the relationship between miR-203 and IAV infection.
format	article
author	Sen Zhang Jing Li Junfeng Li Yinhui Yang Xiaoping Kang Yuchang Li Xiaoyan Wu Qingyu Zhu Yusen Zhou Yi Hu
author_facet	Sen Zhang Jing Li Junfeng Li Yinhui Yang Xiaoping Kang Yuchang Li Xiaoyan Wu Qingyu Zhu Yusen Zhou Yi Hu
author_sort	Sen Zhang
title	Up-regulation of microRNA-203 in influenza A virus infection inhibits viral replication by targeting DR1
title_short	Up-regulation of microRNA-203 in influenza A virus infection inhibits viral replication by targeting DR1
title_full	Up-regulation of microRNA-203 in influenza A virus infection inhibits viral replication by targeting DR1
title_fullStr	Up-regulation of microRNA-203 in influenza A virus infection inhibits viral replication by targeting DR1
title_full_unstemmed	Up-regulation of microRNA-203 in influenza A virus infection inhibits viral replication by targeting DR1
title_sort	up-regulation of microrna-203 in influenza a virus infection inhibits viral replication by targeting dr1
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a944f0ed5d644c42a79792fc2b06c097
work_keys_str_mv	AT senzhang upregulationofmicrorna203ininfluenzaavirusinfectioninhibitsviralreplicationbytargetingdr1 AT jingli upregulationofmicrorna203ininfluenzaavirusinfectioninhibitsviralreplicationbytargetingdr1 AT junfengli upregulationofmicrorna203ininfluenzaavirusinfectioninhibitsviralreplicationbytargetingdr1 AT yinhuiyang upregulationofmicrorna203ininfluenzaavirusinfectioninhibitsviralreplicationbytargetingdr1 AT xiaopingkang upregulationofmicrorna203ininfluenzaavirusinfectioninhibitsviralreplicationbytargetingdr1 AT yuchangli upregulationofmicrorna203ininfluenzaavirusinfectioninhibitsviralreplicationbytargetingdr1 AT xiaoyanwu upregulationofmicrorna203ininfluenzaavirusinfectioninhibitsviralreplicationbytargetingdr1 AT qingyuzhu upregulationofmicrorna203ininfluenzaavirusinfectioninhibitsviralreplicationbytargetingdr1 AT yusenzhou upregulationofmicrorna203ininfluenzaavirusinfectioninhibitsviralreplicationbytargetingdr1 AT yihu upregulationofmicrorna203ininfluenzaavirusinfectioninhibitsviralreplicationbytargetingdr1
_version_	1718384677275828224

Up-regulation of microRNA-203 in influenza A virus infection inhibits viral replication by targeting DR1

Ejemplares similares