Insights into the role of deep-sea squids of the genus Histioteuthis (Histioteuthidae) in the life cycle of ascaridoid parasites in the Central Mediterranean Sea waters

Abstract Ascaridoid nematodes comprise a wide range of heteroxenous parasites infecting top fish predators and marine mammals as definitive hosts, with crustaceans, squids, and fishes acting as intermediate/paratenic hosts. Limited data exist on the species and role of several intermediate and parat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marialetizia Palomba, Simonetta Mattiucci, Fabio Crocetta, David Osca, Mario Santoro
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a945760697b045b6bee0eb95ff4b832e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a945760697b045b6bee0eb95ff4b832e
record_format	dspace
spelling	oai:doaj.org-article:a945760697b045b6bee0eb95ff4b832e2021-12-02T14:25:09ZInsights into the role of deep-sea squids of the genus Histioteuthis (Histioteuthidae) in the life cycle of ascaridoid parasites in the Central Mediterranean Sea waters10.1038/s41598-021-86248-52045-2322https://doaj.org/article/a945760697b045b6bee0eb95ff4b832e2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86248-5https://doaj.org/toc/2045-2322Abstract Ascaridoid nematodes comprise a wide range of heteroxenous parasites infecting top fish predators and marine mammals as definitive hosts, with crustaceans, squids, and fishes acting as intermediate/paratenic hosts. Limited data exist on the species and role of several intermediate and paratenic hosts in the life cycle of these parasites. In the aim of adding knowledge on the role of squid species in their life cycle, we have here investigated the larval ascaridoid nematodes collected from the deep-sea umbrella squid Histioteuthis bonnelli and the reverse jewel squid Histioteuthis reversa captured in the Central Mediterranean Sea (Tyrrhenian Sea). Morphological study and sequence analysis of the internal transcribed spacer (ITS) regions of the ribosomal DNA (rDNA) and the mitochondrial cytochrome c oxidase subunit 2 (mtDNA cox2) gene locus revealed the occurrence of Anisakis physeteris and of an unidentified species of the genus Lappetascaris. Sequence analysis revealed that specimens of Lappetascaris from both squid species matched at 100% sequences previously deposited in GenBank from larval ascaridoids collected in octopuses of the genus Eledone of the Mediterranean Sea. The Bayesian inference tree topology obtained from the analysis of the fragments amplified showed that Lappetascaris specimens were included in a major clade comprising Hysterothylacium species collected in fishes of the families Xiphiidae and Istiophoridae. As regards the site of infection in the squid host species, A. physeteris larvae predominated (60.7%) in the gonads, while those of Lappetascaris (76.3%) were found infecting the mantle musculature. The overall high values of parasitic load suggest both squid species as transmitting hosts of third stage larvae of Lappetascaris to top predator fishes, as well as the umbrella squid as an intermediate/paratenic host in the life cycle of A. physeteris in the Mediterranean Sea.Marialetizia PalombaSimonetta MattiucciFabio CrocettaDavid OscaMario SantoroNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marialetizia Palomba Simonetta Mattiucci Fabio Crocetta David Osca Mario Santoro Insights into the role of deep-sea squids of the genus Histioteuthis (Histioteuthidae) in the life cycle of ascaridoid parasites in the Central Mediterranean Sea waters
description	Abstract Ascaridoid nematodes comprise a wide range of heteroxenous parasites infecting top fish predators and marine mammals as definitive hosts, with crustaceans, squids, and fishes acting as intermediate/paratenic hosts. Limited data exist on the species and role of several intermediate and paratenic hosts in the life cycle of these parasites. In the aim of adding knowledge on the role of squid species in their life cycle, we have here investigated the larval ascaridoid nematodes collected from the deep-sea umbrella squid Histioteuthis bonnelli and the reverse jewel squid Histioteuthis reversa captured in the Central Mediterranean Sea (Tyrrhenian Sea). Morphological study and sequence analysis of the internal transcribed spacer (ITS) regions of the ribosomal DNA (rDNA) and the mitochondrial cytochrome c oxidase subunit 2 (mtDNA cox2) gene locus revealed the occurrence of Anisakis physeteris and of an unidentified species of the genus Lappetascaris. Sequence analysis revealed that specimens of Lappetascaris from both squid species matched at 100% sequences previously deposited in GenBank from larval ascaridoids collected in octopuses of the genus Eledone of the Mediterranean Sea. The Bayesian inference tree topology obtained from the analysis of the fragments amplified showed that Lappetascaris specimens were included in a major clade comprising Hysterothylacium species collected in fishes of the families Xiphiidae and Istiophoridae. As regards the site of infection in the squid host species, A. physeteris larvae predominated (60.7%) in the gonads, while those of Lappetascaris (76.3%) were found infecting the mantle musculature. The overall high values of parasitic load suggest both squid species as transmitting hosts of third stage larvae of Lappetascaris to top predator fishes, as well as the umbrella squid as an intermediate/paratenic host in the life cycle of A. physeteris in the Mediterranean Sea.
format	article
author	Marialetizia Palomba Simonetta Mattiucci Fabio Crocetta David Osca Mario Santoro
author_facet	Marialetizia Palomba Simonetta Mattiucci Fabio Crocetta David Osca Mario Santoro
author_sort	Marialetizia Palomba
title	Insights into the role of deep-sea squids of the genus Histioteuthis (Histioteuthidae) in the life cycle of ascaridoid parasites in the Central Mediterranean Sea waters
title_short	Insights into the role of deep-sea squids of the genus Histioteuthis (Histioteuthidae) in the life cycle of ascaridoid parasites in the Central Mediterranean Sea waters
title_full	Insights into the role of deep-sea squids of the genus Histioteuthis (Histioteuthidae) in the life cycle of ascaridoid parasites in the Central Mediterranean Sea waters
title_fullStr	Insights into the role of deep-sea squids of the genus Histioteuthis (Histioteuthidae) in the life cycle of ascaridoid parasites in the Central Mediterranean Sea waters
title_full_unstemmed	Insights into the role of deep-sea squids of the genus Histioteuthis (Histioteuthidae) in the life cycle of ascaridoid parasites in the Central Mediterranean Sea waters
title_sort	insights into the role of deep-sea squids of the genus histioteuthis (histioteuthidae) in the life cycle of ascaridoid parasites in the central mediterranean sea waters
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a945760697b045b6bee0eb95ff4b832e
work_keys_str_mv	AT marialetiziapalomba insightsintotheroleofdeepseasquidsofthegenushistioteuthishistioteuthidaeinthelifecycleofascaridoidparasitesinthecentralmediterraneanseawaters AT simonettamattiucci insightsintotheroleofdeepseasquidsofthegenushistioteuthishistioteuthidaeinthelifecycleofascaridoidparasitesinthecentralmediterraneanseawaters AT fabiocrocetta insightsintotheroleofdeepseasquidsofthegenushistioteuthishistioteuthidaeinthelifecycleofascaridoidparasitesinthecentralmediterraneanseawaters AT davidosca insightsintotheroleofdeepseasquidsofthegenushistioteuthishistioteuthidaeinthelifecycleofascaridoidparasitesinthecentralmediterraneanseawaters AT mariosantoro insightsintotheroleofdeepseasquidsofthegenushistioteuthishistioteuthidaeinthelifecycleofascaridoidparasitesinthecentralmediterraneanseawaters
_version_	1718391443993657344

Insights into the role of deep-sea squids of the genus Histioteuthis (Histioteuthidae) in the life cycle of ascaridoid parasites in the Central Mediterranean Sea waters

Ejemplares similares