OsFH15, a class I formin, interacts with microfilaments and microtubules to regulate grain size via affecting cell expansion in rice

Abstract Grain size is an important agronomic trait determining rice yield and is mainly restricted by spikelet hull size. However, it remains largely unknown how the spikelet hull size is regulated. In this study, OsFH15, a class I formin protein in Oryza sativa, was found to be able to regulate th...

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Autores principales:	Tiantian Sun, Shanwei Li, Haiyun Ren
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a94e088e2eaf4d60a082849876523ead
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spelling	oai:doaj.org-article:a94e088e2eaf4d60a082849876523ead2021-12-02T11:40:33ZOsFH15, a class I formin, interacts with microfilaments and microtubules to regulate grain size via affecting cell expansion in rice10.1038/s41598-017-06431-52045-2322https://doaj.org/article/a94e088e2eaf4d60a082849876523ead2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06431-5https://doaj.org/toc/2045-2322Abstract Grain size is an important agronomic trait determining rice yield and is mainly restricted by spikelet hull size. However, it remains largely unknown how the spikelet hull size is regulated. In this study, OsFH15, a class I formin protein in Oryza sativa, was found to be able to regulate the size of cells and spikelet hull. OsFH15-Cas9 and OsFH15-RNAi mutants had decreased grain size with reduced cell length, cell width and cell area of inner epidermal cells of the lemma compared with wild-type plants. By contrast, OsFH15-overexpressed plants had increased grain size with larger cells, as well as more abundant microtubules (MTs) and actin filaments (AFs) arrays. OsFH15 was mainly expressed in shoot apical meristem (SAM), spikelets, spikelet hulls and seeds in rice. In vitro biochemical experiments showed that OsFH15 can efficiently nucleate actin polymerization with or without profilin, can cap the barbed end of AFs, and can bind and bundle both AFs and MTs. OsFH15 can also crosslink AFs with MTs, and preferentially bind MTs to AFs. These results demonstrated that OsFH15 played an important role in grain-size control by affecting cell expansion through regulating AFs and MTs.Tiantian SunShanwei LiHaiyun RenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tiantian Sun Shanwei Li Haiyun Ren OsFH15, a class I formin, interacts with microfilaments and microtubules to regulate grain size via affecting cell expansion in rice
description	Abstract Grain size is an important agronomic trait determining rice yield and is mainly restricted by spikelet hull size. However, it remains largely unknown how the spikelet hull size is regulated. In this study, OsFH15, a class I formin protein in Oryza sativa, was found to be able to regulate the size of cells and spikelet hull. OsFH15-Cas9 and OsFH15-RNAi mutants had decreased grain size with reduced cell length, cell width and cell area of inner epidermal cells of the lemma compared with wild-type plants. By contrast, OsFH15-overexpressed plants had increased grain size with larger cells, as well as more abundant microtubules (MTs) and actin filaments (AFs) arrays. OsFH15 was mainly expressed in shoot apical meristem (SAM), spikelets, spikelet hulls and seeds in rice. In vitro biochemical experiments showed that OsFH15 can efficiently nucleate actin polymerization with or without profilin, can cap the barbed end of AFs, and can bind and bundle both AFs and MTs. OsFH15 can also crosslink AFs with MTs, and preferentially bind MTs to AFs. These results demonstrated that OsFH15 played an important role in grain-size control by affecting cell expansion through regulating AFs and MTs.
format	article
author	Tiantian Sun Shanwei Li Haiyun Ren
author_facet	Tiantian Sun Shanwei Li Haiyun Ren
author_sort	Tiantian Sun
title	OsFH15, a class I formin, interacts with microfilaments and microtubules to regulate grain size via affecting cell expansion in rice
title_short	OsFH15, a class I formin, interacts with microfilaments and microtubules to regulate grain size via affecting cell expansion in rice
title_full	OsFH15, a class I formin, interacts with microfilaments and microtubules to regulate grain size via affecting cell expansion in rice
title_fullStr	OsFH15, a class I formin, interacts with microfilaments and microtubules to regulate grain size via affecting cell expansion in rice
title_full_unstemmed	OsFH15, a class I formin, interacts with microfilaments and microtubules to regulate grain size via affecting cell expansion in rice
title_sort	osfh15, a class i formin, interacts with microfilaments and microtubules to regulate grain size via affecting cell expansion in rice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a94e088e2eaf4d60a082849876523ead
work_keys_str_mv	AT tiantiansun osfh15aclassiformininteractswithmicrofilamentsandmicrotubulestoregulategrainsizeviaaffectingcellexpansioninrice AT shanweili osfh15aclassiformininteractswithmicrofilamentsandmicrotubulestoregulategrainsizeviaaffectingcellexpansioninrice AT haiyunren osfh15aclassiformininteractswithmicrofilamentsandmicrotubulestoregulategrainsizeviaaffectingcellexpansioninrice
_version_	1718395600722984960

OsFH15, a class I formin, interacts with microfilaments and microtubules to regulate grain size via affecting cell expansion in rice

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