Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina

Abstract Microalgal photosynthesis provides energy and carbon-containing precursors for the biosynthesis of storage carbohydrates such as starch, chrysolaminarin, lipids, and cell wall components. Under mild nitrogen deficiency (N−), some Nannochloropsis species accumulate lipid by augmenting cytoso...

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Autores principales:	Seok Won Jeong, Seung Won Nam, Kwon HwangBo, Won Joong Jeong, Byeong-ryool Jeong, Yong Keun Chang, Youn-Il Park
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a954ac42f5044a919b208896a3eae673
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spelling	oai:doaj.org-article:a954ac42f5044a919b208896a3eae6732021-12-02T12:32:53ZTranscriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina10.1038/s41598-017-05684-42045-2322https://doaj.org/article/a954ac42f5044a919b208896a3eae6732017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05684-4https://doaj.org/toc/2045-2322Abstract Microalgal photosynthesis provides energy and carbon-containing precursors for the biosynthesis of storage carbohydrates such as starch, chrysolaminarin, lipids, and cell wall components. Under mild nitrogen deficiency (N−), some Nannochloropsis species accumulate lipid by augmenting cytosolic fatty acid biosynthesis with a temporary increase in laminarin. Accordingly, biosynthesis of the cellulose-rich cell wall should change in response to N− stress because this biosynthetic pathway begins with utilisation of the hexose phosphate pool supplied from photosynthesis. However, few studies have characterised microalgal cell wall metabolism, including oleaginous Nannochloropsis sp. microalgae subjected to nitrogen deficiency. Here, we investigated N-induced changes in cellulose biosynthesis in N. salina. We observed that N− induced cell wall thickening, concurrently increased the transcript levels of genes coding for UDPG pyrophosphorylase and cellulose synthases, and increased cellulose content. Nannochloropsis salina cells with thickened cell wall were more susceptible to mechanical stress such as bead-beating and sonication, implicating cellulose metabolism as a potential target for cost-effective microalgal cell disruption.Seok Won JeongSeung Won NamKwon HwangBoWon Joong JeongByeong-ryool JeongYong Keun ChangYoun-Il ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seok Won Jeong Seung Won Nam Kwon HwangBo Won Joong Jeong Byeong-ryool Jeong Yong Keun Chang Youn-Il Park Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina
description	Abstract Microalgal photosynthesis provides energy and carbon-containing precursors for the biosynthesis of storage carbohydrates such as starch, chrysolaminarin, lipids, and cell wall components. Under mild nitrogen deficiency (N−), some Nannochloropsis species accumulate lipid by augmenting cytosolic fatty acid biosynthesis with a temporary increase in laminarin. Accordingly, biosynthesis of the cellulose-rich cell wall should change in response to N− stress because this biosynthetic pathway begins with utilisation of the hexose phosphate pool supplied from photosynthesis. However, few studies have characterised microalgal cell wall metabolism, including oleaginous Nannochloropsis sp. microalgae subjected to nitrogen deficiency. Here, we investigated N-induced changes in cellulose biosynthesis in N. salina. We observed that N− induced cell wall thickening, concurrently increased the transcript levels of genes coding for UDPG pyrophosphorylase and cellulose synthases, and increased cellulose content. Nannochloropsis salina cells with thickened cell wall were more susceptible to mechanical stress such as bead-beating and sonication, implicating cellulose metabolism as a potential target for cost-effective microalgal cell disruption.
format	article
author	Seok Won Jeong Seung Won Nam Kwon HwangBo Won Joong Jeong Byeong-ryool Jeong Yong Keun Chang Youn-Il Park
author_facet	Seok Won Jeong Seung Won Nam Kwon HwangBo Won Joong Jeong Byeong-ryool Jeong Yong Keun Chang Youn-Il Park
author_sort	Seok Won Jeong
title	Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina
title_short	Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina
title_full	Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina
title_fullStr	Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina
title_full_unstemmed	Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina
title_sort	transcriptional regulation of cellulose biosynthesis during the early phase of nitrogen deprivation in nannochloropsis salina
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a954ac42f5044a919b208896a3eae673
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Transcriptional Regulation of Cellulose Biosynthesis during the Early Phase of Nitrogen Deprivation in Nannochloropsis salina

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