An integrated set-up for ex vivo characterisation of biaxial murine artery biomechanics under pulsatile conditions

Abstract Ex vivo characterisation of arterial biomechanics enables detailed discrimination of the various cellular and extracellular contributions to arterial stiffness. However, ex vivo biomechanical studies are commonly performed under quasi-static conditions, whereas dynamic biomechanical behavio...

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Autores principales:	Myrthe M. van der Bruggen, Koen D. Reesink, Paul J. M. Spronck, Nicole Bitsch, Jeroen Hameleers, Remco T. A. Megens, Casper G. Schalkwijk, Tammo Delhaas, Bart Spronck
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a956891e68dd42f689599fab716dc97d
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spelling	oai:doaj.org-article:a956891e68dd42f689599fab716dc97d2021-12-02T13:57:26ZAn integrated set-up for ex vivo characterisation of biaxial murine artery biomechanics under pulsatile conditions10.1038/s41598-021-81151-52045-2322https://doaj.org/article/a956891e68dd42f689599fab716dc97d2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81151-5https://doaj.org/toc/2045-2322Abstract Ex vivo characterisation of arterial biomechanics enables detailed discrimination of the various cellular and extracellular contributions to arterial stiffness. However, ex vivo biomechanical studies are commonly performed under quasi-static conditions, whereas dynamic biomechanical behaviour (as relevant in vivo) may differ substantially. Hence, we aim to (1) develop an integrated set-up for quasi-static and dynamic biaxial biomechanical testing, (2) quantify set-up reproducibility, and (3) illustrate the differences in measured arterial stiffness between quasi-static and dynamic conditions. Twenty-two mouse carotid arteries were mounted between glass micropipettes and kept fully vasodilated. While recording pressure, axial force (F), and inner diameter, arteries were exposed to (1) quasi-static pressure inflation from 0 to 200 mmHg; (2) 300 bpm dynamic pressure inflation (peaking at 80/120/160 mmHg); and (3) axial stretch (λ z ) variation at constant pressures of 10/60/100/140/200 mmHg. Measurements were performed in duplicate. Single-point pulse wave velocities (PWV; Bramwell-Hill) and axial stiffness coefficients (c ax = dF/dλ z ) were calculated at the in vivo value of λ z . Within-subject coefficients of variation were ~ 20%. Dynamic PWVs were consistently higher than quasi-static PWVs (p < 0.001); c ax increased with increasing pressure. We demonstrated the feasibility of ex vivo biomechanical characterisation of biaxially-loaded murine carotid arteries under pulsatile conditions, and quantified reproducibility allowing for well-powered future study design.Myrthe M. van der BruggenKoen D. ReesinkPaul J. M. SpronckNicole BitschJeroen HameleersRemco T. A. MegensCasper G. SchalkwijkTammo DelhaasBart SpronckNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Myrthe M. van der Bruggen Koen D. Reesink Paul J. M. Spronck Nicole Bitsch Jeroen Hameleers Remco T. A. Megens Casper G. Schalkwijk Tammo Delhaas Bart Spronck An integrated set-up for ex vivo characterisation of biaxial murine artery biomechanics under pulsatile conditions
description	Abstract Ex vivo characterisation of arterial biomechanics enables detailed discrimination of the various cellular and extracellular contributions to arterial stiffness. However, ex vivo biomechanical studies are commonly performed under quasi-static conditions, whereas dynamic biomechanical behaviour (as relevant in vivo) may differ substantially. Hence, we aim to (1) develop an integrated set-up for quasi-static and dynamic biaxial biomechanical testing, (2) quantify set-up reproducibility, and (3) illustrate the differences in measured arterial stiffness between quasi-static and dynamic conditions. Twenty-two mouse carotid arteries were mounted between glass micropipettes and kept fully vasodilated. While recording pressure, axial force (F), and inner diameter, arteries were exposed to (1) quasi-static pressure inflation from 0 to 200 mmHg; (2) 300 bpm dynamic pressure inflation (peaking at 80/120/160 mmHg); and (3) axial stretch (λ z ) variation at constant pressures of 10/60/100/140/200 mmHg. Measurements were performed in duplicate. Single-point pulse wave velocities (PWV; Bramwell-Hill) and axial stiffness coefficients (c ax = dF/dλ z ) were calculated at the in vivo value of λ z . Within-subject coefficients of variation were ~ 20%. Dynamic PWVs were consistently higher than quasi-static PWVs (p < 0.001); c ax increased with increasing pressure. We demonstrated the feasibility of ex vivo biomechanical characterisation of biaxially-loaded murine carotid arteries under pulsatile conditions, and quantified reproducibility allowing for well-powered future study design.
format	article
author	Myrthe M. van der Bruggen Koen D. Reesink Paul J. M. Spronck Nicole Bitsch Jeroen Hameleers Remco T. A. Megens Casper G. Schalkwijk Tammo Delhaas Bart Spronck
author_facet	Myrthe M. van der Bruggen Koen D. Reesink Paul J. M. Spronck Nicole Bitsch Jeroen Hameleers Remco T. A. Megens Casper G. Schalkwijk Tammo Delhaas Bart Spronck
author_sort	Myrthe M. van der Bruggen
title	An integrated set-up for ex vivo characterisation of biaxial murine artery biomechanics under pulsatile conditions
title_short	An integrated set-up for ex vivo characterisation of biaxial murine artery biomechanics under pulsatile conditions
title_full	An integrated set-up for ex vivo characterisation of biaxial murine artery biomechanics under pulsatile conditions
title_fullStr	An integrated set-up for ex vivo characterisation of biaxial murine artery biomechanics under pulsatile conditions
title_full_unstemmed	An integrated set-up for ex vivo characterisation of biaxial murine artery biomechanics under pulsatile conditions
title_sort	integrated set-up for ex vivo characterisation of biaxial murine artery biomechanics under pulsatile conditions
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a956891e68dd42f689599fab716dc97d
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An integrated set-up for ex vivo characterisation of biaxial murine artery biomechanics under pulsatile conditions

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