Establishment and evaluation of a Sprague-Dawley rat model of intramedullary spinal cord glioma

Background and Aim: Intramedullary spinal cord glioma has no evident boundary with normal spinal cord tissue. The rate of successful surgical resection of intramedullary spinal cord glioma is low. Well-established animal models for intramedullary spinal cord glioma can help promote translation from...

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Autores principales:	Dongkang Liu, Guo Yi, James Jin Wang, Guihuai Wang
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2021
Materias:	basso beattie and bresnahan intramedullary glioma rat model spinal cord spine cord Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/a95700d206e446ba9b9462b6ef8925d7
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spelling	oai:doaj.org-article:a95700d206e446ba9b9462b6ef8925d72021-11-19T10:42:03ZEstablishment and evaluation of a Sprague-Dawley rat model of intramedullary spinal cord glioma2589-61132589-612110.4103/glioma.glioma_16_21https://doaj.org/article/a95700d206e446ba9b9462b6ef8925d72021-01-01T00:00:00Zhttp://www.jglioma.com/article.asp?issn=2589-6113;year=2021;volume=4;issue=3;spage=42;epage=48;aulast=Liuhttps://doaj.org/toc/2589-6113https://doaj.org/toc/2589-6121Background and Aim: Intramedullary spinal cord glioma has no evident boundary with normal spinal cord tissue. The rate of successful surgical resection of intramedullary spinal cord glioma is low. Well-established animal models for intramedullary spinal cord glioma can help promote translation from related basic therapy research to clinical applications. In this study, we established a rat model of intramedullary spinal cord glioma. Materials and Methods: A total of 23 male Sprague-Dawley (SD) rats were randomized into blank control (n = 3) and experimental (n = 20) groups. The blank control group received intramedullary injection of Dulbecco's modified Eagle medium (DMEM) and the experimental group was injected with DMEM containing C6 glioma cells. The neurological states of these rats were evaluated using the Basso, Beattie, and Bresnahan scale (BBB). Tumor sizes were measured by magnetic resonance imaging. The histopathological analysis was performed to observe the growth of infiltrating tumors. All procedures involving animals were approved by the Ethics Committee of the Laboratory Animal Facility Biomedical Analysis Center, Tsinghua University (Beijing, China; approval No. 17-WGH1). Results: On postoperative 7 days, the experimental group presented with a significant progressive decrease in motor function (mean BBB score 15.00 ± 1.20) compared with the blank control group (20.67 ± 0.47, P < 0.01). All rats in the experimental group showed exponential tumor growth and had an average survival of up to 5 weeks after tumor cell implantation. The tumor sizes were 3.18 ± 0.21 mm3, 68.55 ± 3.38 mm3, and 345.28 ± 22.57 mm3 on postoperative 7, 14, and 28 days. The histopathological analysis illustrated that the growth of infiltrating tumors followed the longitudinal axis of the spinal cord. Conclusions: Thus, we have established a SD rat model of intramedullary spinal cord glioma, and we found that our findings are reproducible and homogeneous. These positive results provide solid bases for further studies of intramedullary spinal cord glioma.Dongkang LiuGuo YiJames Jin WangGuihuai WangWolters Kluwer Medknow Publicationsarticlebassobeattieand bresnahanintramedullary gliomarat modelspinal cordspine cordNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENGlioma, Vol 4, Iss 3, Pp 42-48 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	basso beattie and bresnahan intramedullary glioma rat model spinal cord spine cord Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	basso beattie and bresnahan intramedullary glioma rat model spinal cord spine cord Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Dongkang Liu Guo Yi James Jin Wang Guihuai Wang Establishment and evaluation of a Sprague-Dawley rat model of intramedullary spinal cord glioma
description	Background and Aim: Intramedullary spinal cord glioma has no evident boundary with normal spinal cord tissue. The rate of successful surgical resection of intramedullary spinal cord glioma is low. Well-established animal models for intramedullary spinal cord glioma can help promote translation from related basic therapy research to clinical applications. In this study, we established a rat model of intramedullary spinal cord glioma. Materials and Methods: A total of 23 male Sprague-Dawley (SD) rats were randomized into blank control (n = 3) and experimental (n = 20) groups. The blank control group received intramedullary injection of Dulbecco's modified Eagle medium (DMEM) and the experimental group was injected with DMEM containing C6 glioma cells. The neurological states of these rats were evaluated using the Basso, Beattie, and Bresnahan scale (BBB). Tumor sizes were measured by magnetic resonance imaging. The histopathological analysis was performed to observe the growth of infiltrating tumors. All procedures involving animals were approved by the Ethics Committee of the Laboratory Animal Facility Biomedical Analysis Center, Tsinghua University (Beijing, China; approval No. 17-WGH1). Results: On postoperative 7 days, the experimental group presented with a significant progressive decrease in motor function (mean BBB score 15.00 ± 1.20) compared with the blank control group (20.67 ± 0.47, P < 0.01). All rats in the experimental group showed exponential tumor growth and had an average survival of up to 5 weeks after tumor cell implantation. The tumor sizes were 3.18 ± 0.21 mm3, 68.55 ± 3.38 mm3, and 345.28 ± 22.57 mm3 on postoperative 7, 14, and 28 days. The histopathological analysis illustrated that the growth of infiltrating tumors followed the longitudinal axis of the spinal cord. Conclusions: Thus, we have established a SD rat model of intramedullary spinal cord glioma, and we found that our findings are reproducible and homogeneous. These positive results provide solid bases for further studies of intramedullary spinal cord glioma.
format	article
author	Dongkang Liu Guo Yi James Jin Wang Guihuai Wang
author_facet	Dongkang Liu Guo Yi James Jin Wang Guihuai Wang
author_sort	Dongkang Liu
title	Establishment and evaluation of a Sprague-Dawley rat model of intramedullary spinal cord glioma
title_short	Establishment and evaluation of a Sprague-Dawley rat model of intramedullary spinal cord glioma
title_full	Establishment and evaluation of a Sprague-Dawley rat model of intramedullary spinal cord glioma
title_fullStr	Establishment and evaluation of a Sprague-Dawley rat model of intramedullary spinal cord glioma
title_full_unstemmed	Establishment and evaluation of a Sprague-Dawley rat model of intramedullary spinal cord glioma
title_sort	establishment and evaluation of a sprague-dawley rat model of intramedullary spinal cord glioma
publisher	Wolters Kluwer Medknow Publications
publishDate	2021
url	https://doaj.org/article/a95700d206e446ba9b9462b6ef8925d7
work_keys_str_mv	AT dongkangliu establishmentandevaluationofaspraguedawleyratmodelofintramedullaryspinalcordglioma AT guoyi establishmentandevaluationofaspraguedawleyratmodelofintramedullaryspinalcordglioma AT jamesjinwang establishmentandevaluationofaspraguedawleyratmodelofintramedullaryspinalcordglioma AT guihuaiwang establishmentandevaluationofaspraguedawleyratmodelofintramedullaryspinalcordglioma
_version_	1718420212243496960

Establishment and evaluation of a Sprague-Dawley rat model of intramedullary spinal cord glioma

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