The Spt10 GNAT Superfamily Protein Modulates Development, Cell Cycle Progression and Virulence in the Fungal Insect Pathogen, <i>Beauveria bassiana</i>

Chromatin remodeling is mediated in part by post-translational acetylation/deacetylation modifications of histones. Histone acetyltransferases (HATs), e.g., members of the GNAT/MYST superfamily, activate gene transcription via promotion of euchromatin formation. Here, we characterized a GNAT family...

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Autores principales:	Qing Cai, Juan-Juan Wang, Jia-Tao Xie, Dao-Hong Jiang, Nemat O. Keyhani
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	histone acetyltransferase virulence asexual development cell cycle hyphal septation metabolism Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a95c792fdf2147a4a4206b529d807297
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spelling	oai:doaj.org-article:a95c792fdf2147a4a4206b529d8072972021-11-25T18:05:35ZThe Spt10 GNAT Superfamily Protein Modulates Development, Cell Cycle Progression and Virulence in the Fungal Insect Pathogen, <i>Beauveria bassiana</i>10.3390/jof71109052309-608Xhttps://doaj.org/article/a95c792fdf2147a4a4206b529d8072972021-10-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/905https://doaj.org/toc/2309-608XChromatin remodeling is mediated in part by post-translational acetylation/deacetylation modifications of histones. Histone acetyltransferases (HATs), e.g., members of the GNAT/MYST superfamily, activate gene transcription via promotion of euchromatin formation. Here, we characterized a GNAT family HAT, Spt10 (BbSpt10), in the environmentally and economically important fungal insect pathogen, <i>Beauveria bassiana.</i> Targeted gene knockout of <i>BbSpt10</i> resulted in impaired asexual development and morphogenesis; reduced abilities to utilize various carbon/nitrogen sources; reduced tolerance to heat, fungicides, and DNA damage stress; and attenuated virulence. The Δ<i>BbSpt10</i> mutant showed disrupted cell cycle development and abnormal hyphal septation patterns. Transcriptome analyses of wild type and Δ<i>BbSpt10</i> cells revealed the differential expression of 373 genes, including 153 downregulated and 220 upregulated genes. Bioinformatic analyses revealed downregulated genes to be enriched in pathways involved in amino acid metabolism, cellular transportation, cell type differentiation, and virulence, while upregulated genes were enriched in carbon/nitrogen metabolism, lipid metabolism, DNA process, and cell rescue, defense, and virulence. Downregulated virulence genes included hydrophobins, cellular transporters (ABC and MFS multidrug transporters) and cytochrome P450 detoxification genes. These data indicated broad effects of BbSpt10 on fungal development, multi-stress response, and virulence.Qing CaiJuan-Juan WangJia-Tao XieDao-Hong JiangNemat O. KeyhaniMDPI AGarticlehistone acetyltransferasevirulenceasexual developmentcell cyclehyphal septationmetabolismBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 905, p 905 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	histone acetyltransferase virulence asexual development cell cycle hyphal septation metabolism Biology (General) QH301-705.5
spellingShingle	histone acetyltransferase virulence asexual development cell cycle hyphal septation metabolism Biology (General) QH301-705.5 Qing Cai Juan-Juan Wang Jia-Tao Xie Dao-Hong Jiang Nemat O. Keyhani The Spt10 GNAT Superfamily Protein Modulates Development, Cell Cycle Progression and Virulence in the Fungal Insect Pathogen, <i>Beauveria bassiana</i>
description	Chromatin remodeling is mediated in part by post-translational acetylation/deacetylation modifications of histones. Histone acetyltransferases (HATs), e.g., members of the GNAT/MYST superfamily, activate gene transcription via promotion of euchromatin formation. Here, we characterized a GNAT family HAT, Spt10 (BbSpt10), in the environmentally and economically important fungal insect pathogen, <i>Beauveria bassiana.</i> Targeted gene knockout of <i>BbSpt10</i> resulted in impaired asexual development and morphogenesis; reduced abilities to utilize various carbon/nitrogen sources; reduced tolerance to heat, fungicides, and DNA damage stress; and attenuated virulence. The Δ<i>BbSpt10</i> mutant showed disrupted cell cycle development and abnormal hyphal septation patterns. Transcriptome analyses of wild type and Δ<i>BbSpt10</i> cells revealed the differential expression of 373 genes, including 153 downregulated and 220 upregulated genes. Bioinformatic analyses revealed downregulated genes to be enriched in pathways involved in amino acid metabolism, cellular transportation, cell type differentiation, and virulence, while upregulated genes were enriched in carbon/nitrogen metabolism, lipid metabolism, DNA process, and cell rescue, defense, and virulence. Downregulated virulence genes included hydrophobins, cellular transporters (ABC and MFS multidrug transporters) and cytochrome P450 detoxification genes. These data indicated broad effects of BbSpt10 on fungal development, multi-stress response, and virulence.
format	article
author	Qing Cai Juan-Juan Wang Jia-Tao Xie Dao-Hong Jiang Nemat O. Keyhani
author_facet	Qing Cai Juan-Juan Wang Jia-Tao Xie Dao-Hong Jiang Nemat O. Keyhani
author_sort	Qing Cai
title	The Spt10 GNAT Superfamily Protein Modulates Development, Cell Cycle Progression and Virulence in the Fungal Insect Pathogen, <i>Beauveria bassiana</i>
title_short	The Spt10 GNAT Superfamily Protein Modulates Development, Cell Cycle Progression and Virulence in the Fungal Insect Pathogen, <i>Beauveria bassiana</i>
title_full	The Spt10 GNAT Superfamily Protein Modulates Development, Cell Cycle Progression and Virulence in the Fungal Insect Pathogen, <i>Beauveria bassiana</i>
title_fullStr	The Spt10 GNAT Superfamily Protein Modulates Development, Cell Cycle Progression and Virulence in the Fungal Insect Pathogen, <i>Beauveria bassiana</i>
title_full_unstemmed	The Spt10 GNAT Superfamily Protein Modulates Development, Cell Cycle Progression and Virulence in the Fungal Insect Pathogen, <i>Beauveria bassiana</i>
title_sort	spt10 gnat superfamily protein modulates development, cell cycle progression and virulence in the fungal insect pathogen, <i>beauveria bassiana</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a95c792fdf2147a4a4206b529d807297
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The Spt10 GNAT Superfamily Protein Modulates Development, Cell Cycle Progression and Virulence in the Fungal Insect Pathogen, <i>Beauveria bassiana</i>

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