Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing

Abstract Recent studies have shown that the transcription factor Foxn1, which is expressed in keratinocytes, is involved in the skin wound healing process, yet how Foxn1 functions remains largely unknown. Our latest data indicate that Foxn1 drives skin healing via engagement in re-epithelization and...

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Autores principales:	Anna Kur-Piotrowska, Joanna Bukowska, Marta M. Kopcewicz, Mariola Dietrich, Joanna Nynca, Mariola Slowinska, Barbara Gawronska-Kozak
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a960bdae683b4c4d8d32b395ee357741
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spelling	oai:doaj.org-article:a960bdae683b4c4d8d32b395ee3577412021-12-02T15:08:27ZFoxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing10.1038/s41598-018-23794-52045-2322https://doaj.org/article/a960bdae683b4c4d8d32b395ee3577412018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-23794-5https://doaj.org/toc/2045-2322Abstract Recent studies have shown that the transcription factor Foxn1, which is expressed in keratinocytes, is involved in the skin wound healing process, yet how Foxn1 functions remains largely unknown. Our latest data indicate that Foxn1 drives skin healing via engagement in re-epithelization and the epithelial-mesenchymal transition (EMT) process. In the present study, 2D-DIGE proteomic profiling analysis of in vitro cultured keratinocytes transfected with adenoviral vector carrying Foxn1-GFP or GFP alone (control) revealed forty proteins with differential abundance between the compared groups. Among the proteins with Foxn1-dependent expression, several enable adaptation to hypoxia. Subsequent experiments revealed that hypoxic conditions (1% O2) stimulate endogenous and exogenous (transfected Ad-Foxn1) Foxn1 expression in cultured keratinocytes. A proteomics analysis also identified proteins that can act as a factors controlling the balance between cell proliferation, differentiation and apoptosis in response to Foxn1. We also showed that in C57BL/6 keratinocytes, the stimulation of Foxn1 by hypoxia is accompanied by increases in Mmp-9 expression. These data corroborate the detected co-localization of Foxn1 and Mmp-9 expression in vivo in post-wounding skin samples of Foxn1::Egfp transgenic mice. Together, our data indicate that Foxn1 orchestrates cellular changes in keratinocytes in both physiological (self-renewal) and pathological (skin wound healing) contexts.Anna Kur-PiotrowskaJoanna BukowskaMarta M. KopcewiczMariola DietrichJoanna NyncaMariola SlowinskaBarbara Gawronska-KozakNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna Kur-Piotrowska Joanna Bukowska Marta M. Kopcewicz Mariola Dietrich Joanna Nynca Mariola Slowinska Barbara Gawronska-Kozak Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing
description	Abstract Recent studies have shown that the transcription factor Foxn1, which is expressed in keratinocytes, is involved in the skin wound healing process, yet how Foxn1 functions remains largely unknown. Our latest data indicate that Foxn1 drives skin healing via engagement in re-epithelization and the epithelial-mesenchymal transition (EMT) process. In the present study, 2D-DIGE proteomic profiling analysis of in vitro cultured keratinocytes transfected with adenoviral vector carrying Foxn1-GFP or GFP alone (control) revealed forty proteins with differential abundance between the compared groups. Among the proteins with Foxn1-dependent expression, several enable adaptation to hypoxia. Subsequent experiments revealed that hypoxic conditions (1% O2) stimulate endogenous and exogenous (transfected Ad-Foxn1) Foxn1 expression in cultured keratinocytes. A proteomics analysis also identified proteins that can act as a factors controlling the balance between cell proliferation, differentiation and apoptosis in response to Foxn1. We also showed that in C57BL/6 keratinocytes, the stimulation of Foxn1 by hypoxia is accompanied by increases in Mmp-9 expression. These data corroborate the detected co-localization of Foxn1 and Mmp-9 expression in vivo in post-wounding skin samples of Foxn1::Egfp transgenic mice. Together, our data indicate that Foxn1 orchestrates cellular changes in keratinocytes in both physiological (self-renewal) and pathological (skin wound healing) contexts.
format	article
author	Anna Kur-Piotrowska Joanna Bukowska Marta M. Kopcewicz Mariola Dietrich Joanna Nynca Mariola Slowinska Barbara Gawronska-Kozak
author_facet	Anna Kur-Piotrowska Joanna Bukowska Marta M. Kopcewicz Mariola Dietrich Joanna Nynca Mariola Slowinska Barbara Gawronska-Kozak
author_sort	Anna Kur-Piotrowska
title	Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing
title_short	Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing
title_full	Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing
title_fullStr	Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing
title_full_unstemmed	Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing
title_sort	foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a960bdae683b4c4d8d32b395ee357741
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Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing

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