Identification of mouse serum miRNA endogenous references by global gene expression profiles.

MicroRNAs (miRNAs) are recently discovered small non-coding RNAs and can serve as serum biomarkers for disease diagnosis and prognoses. Lack of reliable serum miRNA endogenous references for normalization in miRNA gene expression makes single miRNA assays inaccurate. Using TaqMan® real-time PCR miRN...

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Autores principales:	Qing-Sheng Mi, Matthew Weiland, Rui-Qun Qi, Xing-Hua Gao, Laila M Poisson, Li Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a96387182f5a4be0be9f63eafa0fa726
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spelling	oai:doaj.org-article:a96387182f5a4be0be9f63eafa0fa7262021-11-18T07:28:30ZIdentification of mouse serum miRNA endogenous references by global gene expression profiles.1932-620310.1371/journal.pone.0031278https://doaj.org/article/a96387182f5a4be0be9f63eafa0fa7262012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22348064/?tool=EBIhttps://doaj.org/toc/1932-6203MicroRNAs (miRNAs) are recently discovered small non-coding RNAs and can serve as serum biomarkers for disease diagnosis and prognoses. Lack of reliable serum miRNA endogenous references for normalization in miRNA gene expression makes single miRNA assays inaccurate. Using TaqMan® real-time PCR miRNA arrays with a global gene expression normalization strategy, we have analyzed serum miRNA expression profiles of 20 female mice of NOD/ShiLtJ (n = 8), NOR/LtJ (n = 6), and C57BL/6J (n = 6) at different ages and disease conditions. We identified five miRNAs, miR-146a, miR-16, miR-195, miR-30e and miR-744, to be stably expressed in all strains, which could serve as mouse serum miRNA endogenous references for single assay experiments.Qing-Sheng MiMatthew WeilandRui-Qun QiXing-Hua GaoLaila M PoissonLi ZhouPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31278 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qing-Sheng Mi Matthew Weiland Rui-Qun Qi Xing-Hua Gao Laila M Poisson Li Zhou Identification of mouse serum miRNA endogenous references by global gene expression profiles.
description	MicroRNAs (miRNAs) are recently discovered small non-coding RNAs and can serve as serum biomarkers for disease diagnosis and prognoses. Lack of reliable serum miRNA endogenous references for normalization in miRNA gene expression makes single miRNA assays inaccurate. Using TaqMan® real-time PCR miRNA arrays with a global gene expression normalization strategy, we have analyzed serum miRNA expression profiles of 20 female mice of NOD/ShiLtJ (n = 8), NOR/LtJ (n = 6), and C57BL/6J (n = 6) at different ages and disease conditions. We identified five miRNAs, miR-146a, miR-16, miR-195, miR-30e and miR-744, to be stably expressed in all strains, which could serve as mouse serum miRNA endogenous references for single assay experiments.
format	article
author	Qing-Sheng Mi Matthew Weiland Rui-Qun Qi Xing-Hua Gao Laila M Poisson Li Zhou
author_facet	Qing-Sheng Mi Matthew Weiland Rui-Qun Qi Xing-Hua Gao Laila M Poisson Li Zhou
author_sort	Qing-Sheng Mi
title	Identification of mouse serum miRNA endogenous references by global gene expression profiles.
title_short	Identification of mouse serum miRNA endogenous references by global gene expression profiles.
title_full	Identification of mouse serum miRNA endogenous references by global gene expression profiles.
title_fullStr	Identification of mouse serum miRNA endogenous references by global gene expression profiles.
title_full_unstemmed	Identification of mouse serum miRNA endogenous references by global gene expression profiles.
title_sort	identification of mouse serum mirna endogenous references by global gene expression profiles.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/a96387182f5a4be0be9f63eafa0fa726
work_keys_str_mv	AT qingshengmi identificationofmouseserummirnaendogenousreferencesbyglobalgeneexpressionprofiles AT matthewweiland identificationofmouseserummirnaendogenousreferencesbyglobalgeneexpressionprofiles AT ruiqunqi identificationofmouseserummirnaendogenousreferencesbyglobalgeneexpressionprofiles AT xinghuagao identificationofmouseserummirnaendogenousreferencesbyglobalgeneexpressionprofiles AT lailampoisson identificationofmouseserummirnaendogenousreferencesbyglobalgeneexpressionprofiles AT lizhou identificationofmouseserummirnaendogenousreferencesbyglobalgeneexpressionprofiles
_version_	1718423429378473984

Identification of mouse serum miRNA endogenous references by global gene expression profiles.

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