Anti-obesity sodium tungstate treatment triggers axonal and glial plasticity in hypothalamic feeding centers.

<h4>Objective</h4>This study aims at exploring the effects of sodium tungstate treatment on hypothalamic plasticity, which is known to have an important role in the control of energy metabolism.<h4>Methods</h4>Adult lean and high-fat diet-induced obese mice were orally treate...

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Autores principales:	Marta Amigó-Correig, Sílvia Barceló-Batllori, Guadalupe Soria, Alice Krezymon, Alexandre Benani, Luc Pénicaud, Raúl Tudela, Anna Maria Planas, Eduardo Fernández, Maria del Carmen Carmona, Ramon Gomis
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a964716b441a4f29992ed38d91968856
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spelling	oai:doaj.org-article:a964716b441a4f29992ed38d919688562021-11-18T07:13:28ZAnti-obesity sodium tungstate treatment triggers axonal and glial plasticity in hypothalamic feeding centers.1932-620310.1371/journal.pone.0039087https://doaj.org/article/a964716b441a4f29992ed38d919688562012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22802935/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>This study aims at exploring the effects of sodium tungstate treatment on hypothalamic plasticity, which is known to have an important role in the control of energy metabolism.<h4>Methods</h4>Adult lean and high-fat diet-induced obese mice were orally treated with sodium tungstate. Arcuate and paraventricular nuclei and lateral hypothalamus were separated and subjected to proteomic analysis by DIGE and mass spectrometry. Immunohistochemistry and in vivo magnetic resonance imaging were also performed.<h4>Results</h4>Sodium tungstate treatment reduced body weight gain, food intake, and blood glucose and triglyceride levels. These effects were associated with transcriptional and functional changes in the hypothalamus. Proteomic analysis revealed that sodium tungstate modified the expression levels of proteins involved in cell morphology, axonal growth, and tissue remodeling, such as actin, CRMP2 and neurofilaments, and of proteins related to energy metabolism. Moreover, immunohistochemistry studies confirmed results for some targets and further revealed tungstate-dependent regulation of SNAP25 and HPC-1 proteins, suggesting an effect on synaptogenesis as well. Functional test for cell activity based on c-fos-positive cell counting also suggested that sodium tungstate modified hypothalamic basal activity. Finally, in vivo magnetic resonance imaging showed that tungstate treatment can affect neuronal organization in the hypothalamus.<h4>Conclusions</h4>Altogether, these results suggest that sodium tungstate regulates proteins involved in axonal and glial plasticity. The fact that sodium tungstate could modulate hypothalamic plasticity and networks in adulthood makes it a possible and interesting therapeutic strategy not only for obesity management, but also for other neurodegenerative illnesses like Alzheimer's disease.Marta Amigó-CorreigSílvia Barceló-BatlloriGuadalupe SoriaAlice KrezymonAlexandre BenaniLuc PénicaudRaúl TudelaAnna Maria PlanasEduardo FernándezMaria del Carmen CarmonaRamon GomisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e39087 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marta Amigó-Correig Sílvia Barceló-Batllori Guadalupe Soria Alice Krezymon Alexandre Benani Luc Pénicaud Raúl Tudela Anna Maria Planas Eduardo Fernández Maria del Carmen Carmona Ramon Gomis Anti-obesity sodium tungstate treatment triggers axonal and glial plasticity in hypothalamic feeding centers.
description	<h4>Objective</h4>This study aims at exploring the effects of sodium tungstate treatment on hypothalamic plasticity, which is known to have an important role in the control of energy metabolism.<h4>Methods</h4>Adult lean and high-fat diet-induced obese mice were orally treated with sodium tungstate. Arcuate and paraventricular nuclei and lateral hypothalamus were separated and subjected to proteomic analysis by DIGE and mass spectrometry. Immunohistochemistry and in vivo magnetic resonance imaging were also performed.<h4>Results</h4>Sodium tungstate treatment reduced body weight gain, food intake, and blood glucose and triglyceride levels. These effects were associated with transcriptional and functional changes in the hypothalamus. Proteomic analysis revealed that sodium tungstate modified the expression levels of proteins involved in cell morphology, axonal growth, and tissue remodeling, such as actin, CRMP2 and neurofilaments, and of proteins related to energy metabolism. Moreover, immunohistochemistry studies confirmed results for some targets and further revealed tungstate-dependent regulation of SNAP25 and HPC-1 proteins, suggesting an effect on synaptogenesis as well. Functional test for cell activity based on c-fos-positive cell counting also suggested that sodium tungstate modified hypothalamic basal activity. Finally, in vivo magnetic resonance imaging showed that tungstate treatment can affect neuronal organization in the hypothalamus.<h4>Conclusions</h4>Altogether, these results suggest that sodium tungstate regulates proteins involved in axonal and glial plasticity. The fact that sodium tungstate could modulate hypothalamic plasticity and networks in adulthood makes it a possible and interesting therapeutic strategy not only for obesity management, but also for other neurodegenerative illnesses like Alzheimer's disease.
format	article
author	Marta Amigó-Correig Sílvia Barceló-Batllori Guadalupe Soria Alice Krezymon Alexandre Benani Luc Pénicaud Raúl Tudela Anna Maria Planas Eduardo Fernández Maria del Carmen Carmona Ramon Gomis
author_facet	Marta Amigó-Correig Sílvia Barceló-Batllori Guadalupe Soria Alice Krezymon Alexandre Benani Luc Pénicaud Raúl Tudela Anna Maria Planas Eduardo Fernández Maria del Carmen Carmona Ramon Gomis
author_sort	Marta Amigó-Correig
title	Anti-obesity sodium tungstate treatment triggers axonal and glial plasticity in hypothalamic feeding centers.
title_short	Anti-obesity sodium tungstate treatment triggers axonal and glial plasticity in hypothalamic feeding centers.
title_full	Anti-obesity sodium tungstate treatment triggers axonal and glial plasticity in hypothalamic feeding centers.
title_fullStr	Anti-obesity sodium tungstate treatment triggers axonal and glial plasticity in hypothalamic feeding centers.
title_full_unstemmed	Anti-obesity sodium tungstate treatment triggers axonal and glial plasticity in hypothalamic feeding centers.
title_sort	anti-obesity sodium tungstate treatment triggers axonal and glial plasticity in hypothalamic feeding centers.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/a964716b441a4f29992ed38d91968856
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Anti-obesity sodium tungstate treatment triggers axonal and glial plasticity in hypothalamic feeding centers.

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