Involvement of the miR156/SPL module in flooding response in Medicago sativa

Abstract The highly conserved plant microRNA, miR156, affects plant development, metabolite composition, and stress response. Our previous research revealed the role of miR156 in abiotic stress response in Medicago sativa exerted by downregulating SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE transcription...

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Autores principales:	Biruk A. Feyissa, Lisa Amyot, Vida Nasrollahi, Yousef Papadopoulos, Susanne E. Kohalmi, Abdelali Hannoufa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a964fb14641e4d358983f1dc151183fe
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spelling	oai:doaj.org-article:a964fb14641e4d358983f1dc151183fe2021-12-02T10:44:21ZInvolvement of the miR156/SPL module in flooding response in Medicago sativa10.1038/s41598-021-82450-72045-2322https://doaj.org/article/a964fb14641e4d358983f1dc151183fe2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82450-7https://doaj.org/toc/2045-2322Abstract The highly conserved plant microRNA, miR156, affects plant development, metabolite composition, and stress response. Our previous research revealed the role of miR156 in abiotic stress response in Medicago sativa exerted by downregulating SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE transcription factors. Here we investigated the involvement and possible mechanism of action of the miR156/SPL module in flooding tolerance in alfalfa. For that, we used miR156 overexpressing, SPL13RNAi, flood-tolerant (AAC-Trueman) and -sensitive (AC-Caribou) alfalfa cultivars exposed to flooding. We also used Arabidopsis ABA insensitive (abi1-2, abi5-8) mutants and transgenic lines with either overexpressed (KIN10-OX1, KIN10-OX2) or silenced (KIN10RNAi-1, KIN10RNAi-2) catalytic subunit of SnRK1 to investigate a possible role of ABA and SnRK1 in regulating miR156 expression under flooding. Physiological analysis, hormone profiling and global transcriptome changes revealed a role for miR156/SPL module in flooding tolerance. We also identified nine novel alfalfa SPLs (SPL1, SPL1a, SPL2a, SPL7, SPL7a, SPL8, SPL13a, SPL14, SPL16) responsive to flooding. Our results also showed a possible ABA-dependent SnRK1 upregulation to enhance miR156 expression, resulting in downregulation of SPL4, SPL7a, SPL8, SPL9, SPL13, and SPL13a. We conclude that these effects induce flooding adaptive responses in alfalfa and modulate stress physiology by affecting the transcriptome, ABA metabolites and secondary metabolism.Biruk A. FeyissaLisa AmyotVida NasrollahiYousef PapadopoulosSusanne E. KohalmiAbdelali HannoufaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Biruk A. Feyissa Lisa Amyot Vida Nasrollahi Yousef Papadopoulos Susanne E. Kohalmi Abdelali Hannoufa Involvement of the miR156/SPL module in flooding response in Medicago sativa
description	Abstract The highly conserved plant microRNA, miR156, affects plant development, metabolite composition, and stress response. Our previous research revealed the role of miR156 in abiotic stress response in Medicago sativa exerted by downregulating SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE transcription factors. Here we investigated the involvement and possible mechanism of action of the miR156/SPL module in flooding tolerance in alfalfa. For that, we used miR156 overexpressing, SPL13RNAi, flood-tolerant (AAC-Trueman) and -sensitive (AC-Caribou) alfalfa cultivars exposed to flooding. We also used Arabidopsis ABA insensitive (abi1-2, abi5-8) mutants and transgenic lines with either overexpressed (KIN10-OX1, KIN10-OX2) or silenced (KIN10RNAi-1, KIN10RNAi-2) catalytic subunit of SnRK1 to investigate a possible role of ABA and SnRK1 in regulating miR156 expression under flooding. Physiological analysis, hormone profiling and global transcriptome changes revealed a role for miR156/SPL module in flooding tolerance. We also identified nine novel alfalfa SPLs (SPL1, SPL1a, SPL2a, SPL7, SPL7a, SPL8, SPL13a, SPL14, SPL16) responsive to flooding. Our results also showed a possible ABA-dependent SnRK1 upregulation to enhance miR156 expression, resulting in downregulation of SPL4, SPL7a, SPL8, SPL9, SPL13, and SPL13a. We conclude that these effects induce flooding adaptive responses in alfalfa and modulate stress physiology by affecting the transcriptome, ABA metabolites and secondary metabolism.
format	article
author	Biruk A. Feyissa Lisa Amyot Vida Nasrollahi Yousef Papadopoulos Susanne E. Kohalmi Abdelali Hannoufa
author_facet	Biruk A. Feyissa Lisa Amyot Vida Nasrollahi Yousef Papadopoulos Susanne E. Kohalmi Abdelali Hannoufa
author_sort	Biruk A. Feyissa
title	Involvement of the miR156/SPL module in flooding response in Medicago sativa
title_short	Involvement of the miR156/SPL module in flooding response in Medicago sativa
title_full	Involvement of the miR156/SPL module in flooding response in Medicago sativa
title_fullStr	Involvement of the miR156/SPL module in flooding response in Medicago sativa
title_full_unstemmed	Involvement of the miR156/SPL module in flooding response in Medicago sativa
title_sort	involvement of the mir156/spl module in flooding response in medicago sativa
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a964fb14641e4d358983f1dc151183fe
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_version_	1718396782715600896

Involvement of the miR156/SPL module in flooding response in Medicago sativa

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