Validation of baboon pluripotent cells as a model for translational stem cell research

Translation of stem cell therapies to the clinic will be most successful following optimization of efficacy and safety in appropriate preclinical model systems. Among available models, nonhuman primates (NHPs) provide the most accurate recapitulation of human anatomy, physiology, genetics and epigen...

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Autores principales:	Megan A. Mahlke, Keren Cheng, Bo Li, Shital Chaudhari, Christopher S. Navara, John R. McCarrey
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Nonhuman primate Model system Embryonic stem cells Induced pluripotent stem cells Preclinical validation of stem cell therapies Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a969653549e44c7ba28724dcb7160c0a
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spelling	oai:doaj.org-article:a969653549e44c7ba28724dcb7160c0a2021-12-04T04:33:27ZValidation of baboon pluripotent cells as a model for translational stem cell research1873-506110.1016/j.scr.2021.102598https://doaj.org/article/a969653549e44c7ba28724dcb7160c0a2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1873506121004451https://doaj.org/toc/1873-5061Translation of stem cell therapies to the clinic will be most successful following optimization of efficacy and safety in appropriate preclinical model systems. Among available models, nonhuman primates (NHPs) provide the most accurate recapitulation of human anatomy, physiology, genetics and epigenetics. Here, we show that baboon pluripotent cells (PSCs) recapitulate key molecular features of human PSCs with greater accuracy than that found in PSCs from non-primate species such as mice. Specifically, baboon and human PSCs exhibit greater conservation of gene expression patterns, higher sequence and structural homology among pluripotency factors, more equivalent genome-wide patterns of histone and DNA methylation modifications, and similar maintenance of bivalent programming of developmental genes than that found between human and non-primate PSCs.Megan A. MahlkeKeren ChengBo LiShital ChaudhariChristopher S. NavaraJohn R. McCarreyElsevierarticleNonhuman primateModel systemEmbryonic stem cellsInduced pluripotent stem cellsPreclinical validation of stem cell therapiesBiology (General)QH301-705.5ENStem Cell Research, Vol 57, Iss , Pp 102598- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nonhuman primate Model system Embryonic stem cells Induced pluripotent stem cells Preclinical validation of stem cell therapies Biology (General) QH301-705.5
spellingShingle	Nonhuman primate Model system Embryonic stem cells Induced pluripotent stem cells Preclinical validation of stem cell therapies Biology (General) QH301-705.5 Megan A. Mahlke Keren Cheng Bo Li Shital Chaudhari Christopher S. Navara John R. McCarrey Validation of baboon pluripotent cells as a model for translational stem cell research
description	Translation of stem cell therapies to the clinic will be most successful following optimization of efficacy and safety in appropriate preclinical model systems. Among available models, nonhuman primates (NHPs) provide the most accurate recapitulation of human anatomy, physiology, genetics and epigenetics. Here, we show that baboon pluripotent cells (PSCs) recapitulate key molecular features of human PSCs with greater accuracy than that found in PSCs from non-primate species such as mice. Specifically, baboon and human PSCs exhibit greater conservation of gene expression patterns, higher sequence and structural homology among pluripotency factors, more equivalent genome-wide patterns of histone and DNA methylation modifications, and similar maintenance of bivalent programming of developmental genes than that found between human and non-primate PSCs.
format	article
author	Megan A. Mahlke Keren Cheng Bo Li Shital Chaudhari Christopher S. Navara John R. McCarrey
author_facet	Megan A. Mahlke Keren Cheng Bo Li Shital Chaudhari Christopher S. Navara John R. McCarrey
author_sort	Megan A. Mahlke
title	Validation of baboon pluripotent cells as a model for translational stem cell research
title_short	Validation of baboon pluripotent cells as a model for translational stem cell research
title_full	Validation of baboon pluripotent cells as a model for translational stem cell research
title_fullStr	Validation of baboon pluripotent cells as a model for translational stem cell research
title_full_unstemmed	Validation of baboon pluripotent cells as a model for translational stem cell research
title_sort	validation of baboon pluripotent cells as a model for translational stem cell research
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/a969653549e44c7ba28724dcb7160c0a
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Validation of baboon pluripotent cells as a model for translational stem cell research

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