Morphological evaluation using MRI of the olfactory filaments (fila) in a post-traumatic olfactory rat model

Objective: To investigate whether magnetic resonance imaging (MRI) can be used to directly assess olfactory bulb (OB) lesions and quantify the associated morphological changes of olfactory filaments (OF), also known as fila, in an in vivo OB-lesion rat model of the brain. Methods: A surgical group (...

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Autores principales:	Zhi-Fu Sun, Xing Gao, Jayant M. Pinto, Yin He, QingXian Yang, Jun Tian, Qian-Wen Lv, Yong-Xiang Wei
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2018
Materias:	Otorhinolaryngology RF1-547 Surgery RD1-811
Acceso en línea:	https://doaj.org/article/a976d889a92d43fa876f1e8b51a37f17
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spelling	oai:doaj.org-article:a976d889a92d43fa876f1e8b51a37f172021-12-02T11:49:15ZMorphological evaluation using MRI of the olfactory filaments (fila) in a post-traumatic olfactory rat model2095-881110.1016/j.wjorl.2018.03.004https://doaj.org/article/a976d889a92d43fa876f1e8b51a37f172018-03-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2095881118300246https://doaj.org/toc/2095-8811Objective: To investigate whether magnetic resonance imaging (MRI) can be used to directly assess olfactory bulb (OB) lesions and quantify the associated morphological changes of olfactory filaments (OF), also known as fila, in an in vivo OB-lesion rat model of the brain. Methods: A surgical group (n = 5) of male Sprague–Dawley rats was subjected to the unilateral damage of the OB by a steel needle. The control group (n = 5) did not receive surgery. To assess olfactory system injury in vivo, T2-weighted MRI images were acquired in an oblique plane at a 30° angle from transverse plane one day after surgery. These brain regions were also assessed in the controls. The olfactory function was evaluated using the buried food pellet test (BFPT) 5 days before and after surgery. Results: The OF could be clearly observed on the MRI images from all animals. The left and right OF mean lengths (mm) were similar in the control group (0.81 ± 0.18 vs 0.89 ± 0.17, P > 0.05). In the surgical group, the OB was partially injured in all rats. These rats did not show differences in OF length between left- and right-side (0.83 ± 0.18 vs 0.93 ± 0.24, P > 0.05) at the time of measurement. The time (sec) required to find the food pellets in the BFPT was longer after than before the surgery (83.80 ± 34.37 vs 231.44 ± 53.23, P < 0.05). Conclusions: MicroMRI may be a feasible tool to evaluate the OF and OBs in rat models. The unilateral partial OB lesion model appears to be an effective post-traumatic olfactory dysfunction model. Keywords: MicroMRI, Olfactory fila, Olfactory bulb, Post-traumatic olfactory dysfunction model, RatZhi-Fu SunXing GaoJayant M. PintoYin HeQingXian YangJun TianQian-Wen LvYong-Xiang WeiKeAi Communications Co., Ltd.articleOtorhinolaryngologyRF1-547SurgeryRD1-811ENWorld Journal of Otorhinolaryngology-Head and Neck Surgery, Vol 4, Iss 1, Pp 50-56 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Otorhinolaryngology RF1-547 Surgery RD1-811
spellingShingle	Otorhinolaryngology RF1-547 Surgery RD1-811 Zhi-Fu Sun Xing Gao Jayant M. Pinto Yin He QingXian Yang Jun Tian Qian-Wen Lv Yong-Xiang Wei Morphological evaluation using MRI of the olfactory filaments (fila) in a post-traumatic olfactory rat model
description	Objective: To investigate whether magnetic resonance imaging (MRI) can be used to directly assess olfactory bulb (OB) lesions and quantify the associated morphological changes of olfactory filaments (OF), also known as fila, in an in vivo OB-lesion rat model of the brain. Methods: A surgical group (n = 5) of male Sprague–Dawley rats was subjected to the unilateral damage of the OB by a steel needle. The control group (n = 5) did not receive surgery. To assess olfactory system injury in vivo, T2-weighted MRI images were acquired in an oblique plane at a 30° angle from transverse plane one day after surgery. These brain regions were also assessed in the controls. The olfactory function was evaluated using the buried food pellet test (BFPT) 5 days before and after surgery. Results: The OF could be clearly observed on the MRI images from all animals. The left and right OF mean lengths (mm) were similar in the control group (0.81 ± 0.18 vs 0.89 ± 0.17, P > 0.05). In the surgical group, the OB was partially injured in all rats. These rats did not show differences in OF length between left- and right-side (0.83 ± 0.18 vs 0.93 ± 0.24, P > 0.05) at the time of measurement. The time (sec) required to find the food pellets in the BFPT was longer after than before the surgery (83.80 ± 34.37 vs 231.44 ± 53.23, P < 0.05). Conclusions: MicroMRI may be a feasible tool to evaluate the OF and OBs in rat models. The unilateral partial OB lesion model appears to be an effective post-traumatic olfactory dysfunction model. Keywords: MicroMRI, Olfactory fila, Olfactory bulb, Post-traumatic olfactory dysfunction model, Rat
format	article
author	Zhi-Fu Sun Xing Gao Jayant M. Pinto Yin He QingXian Yang Jun Tian Qian-Wen Lv Yong-Xiang Wei
author_facet	Zhi-Fu Sun Xing Gao Jayant M. Pinto Yin He QingXian Yang Jun Tian Qian-Wen Lv Yong-Xiang Wei
author_sort	Zhi-Fu Sun
title	Morphological evaluation using MRI of the olfactory filaments (fila) in a post-traumatic olfactory rat model
title_short	Morphological evaluation using MRI of the olfactory filaments (fila) in a post-traumatic olfactory rat model
title_full	Morphological evaluation using MRI of the olfactory filaments (fila) in a post-traumatic olfactory rat model
title_fullStr	Morphological evaluation using MRI of the olfactory filaments (fila) in a post-traumatic olfactory rat model
title_full_unstemmed	Morphological evaluation using MRI of the olfactory filaments (fila) in a post-traumatic olfactory rat model
title_sort	morphological evaluation using mri of the olfactory filaments (fila) in a post-traumatic olfactory rat model
publisher	KeAi Communications Co., Ltd.
publishDate	2018
url	https://doaj.org/article/a976d889a92d43fa876f1e8b51a37f17
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Morphological evaluation using MRI of the olfactory filaments (fila) in a post-traumatic olfactory rat model

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