Highly Sensitive Whole-Cell Biosensor for Cadmium Detection Based on a Negative Feedback Circuit

Although many whole-cell biosensors (WCBs) for the detection of Cd2+ have been developed over the years, most lack sensitivity and specificity. In this paper, we developed a Cd2+ WCB with a negative feedback amplifier in P. putida KT2440. Based on the slope of the linear detection curve as a measure...

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Autores principales:	Guangbao Zhang, Shuting Hu, Xiaoqiang Jia
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	cadmium detection negative feedback amplifier sensitivity specificity whole-cell biosensor Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/a9785158a9e04c0888ac9772f0c23e81
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spelling	oai:doaj.org-article:a9785158a9e04c0888ac9772f0c23e812021-12-03T06:32:08ZHighly Sensitive Whole-Cell Biosensor for Cadmium Detection Based on a Negative Feedback Circuit2296-418510.3389/fbioe.2021.799781https://doaj.org/article/a9785158a9e04c0888ac9772f0c23e812021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.799781/fullhttps://doaj.org/toc/2296-4185Although many whole-cell biosensors (WCBs) for the detection of Cd2+ have been developed over the years, most lack sensitivity and specificity. In this paper, we developed a Cd2+ WCB with a negative feedback amplifier in P. putida KT2440. Based on the slope of the linear detection curve as a measure of sensitivity, WCB with negative feedback amplifier greatly increased the output signal of the reporter mCherry, resulting in 33% greater sensitivity than in an equivalent WCB without the negative feedback circuit. Moreover, WCB with negative feedback amplifier exhibited increased Cd2+ tolerance and a lower detection limit of 0.1 nM, a remarkable 400-fold improvement compared to the WCB without the negative feedback circuit, which is significantly below the World Health Organization standard of 27 nM (0.003 mg/L) for cadmium in drinking water. Due to the superior amplification of the output signal, WCB with negative feedback amplifier can provide a detectable signal in a much shorter time, and a fast response is highly preferable for real field applications. In addition, the WCB with negative feedback amplifier showed an unusually high specificity for Cd2+ compared to other metal ions, giving signals with other metals that were between 17.6 and 41.4 times weaker than with Cd2+. In summary, the negative feedback amplifier WCB designed in this work meets the requirements of Cd2+ detection with very high sensitivity and specificity, which also demonstrates that genetic negative feedback amplifiers are excellent tools for improving the performance of WCBs.Guangbao ZhangShuting HuXiaoqiang JiaXiaoqiang JiaXiaoqiang JiaFrontiers Media S.A.articlecadmium detectionnegative feedback amplifiersensitivityspecificitywhole-cell biosensorBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cadmium detection negative feedback amplifier sensitivity specificity whole-cell biosensor Biotechnology TP248.13-248.65
spellingShingle	cadmium detection negative feedback amplifier sensitivity specificity whole-cell biosensor Biotechnology TP248.13-248.65 Guangbao Zhang Shuting Hu Xiaoqiang Jia Xiaoqiang Jia Xiaoqiang Jia Highly Sensitive Whole-Cell Biosensor for Cadmium Detection Based on a Negative Feedback Circuit
description	Although many whole-cell biosensors (WCBs) for the detection of Cd2+ have been developed over the years, most lack sensitivity and specificity. In this paper, we developed a Cd2+ WCB with a negative feedback amplifier in P. putida KT2440. Based on the slope of the linear detection curve as a measure of sensitivity, WCB with negative feedback amplifier greatly increased the output signal of the reporter mCherry, resulting in 33% greater sensitivity than in an equivalent WCB without the negative feedback circuit. Moreover, WCB with negative feedback amplifier exhibited increased Cd2+ tolerance and a lower detection limit of 0.1 nM, a remarkable 400-fold improvement compared to the WCB without the negative feedback circuit, which is significantly below the World Health Organization standard of 27 nM (0.003 mg/L) for cadmium in drinking water. Due to the superior amplification of the output signal, WCB with negative feedback amplifier can provide a detectable signal in a much shorter time, and a fast response is highly preferable for real field applications. In addition, the WCB with negative feedback amplifier showed an unusually high specificity for Cd2+ compared to other metal ions, giving signals with other metals that were between 17.6 and 41.4 times weaker than with Cd2+. In summary, the negative feedback amplifier WCB designed in this work meets the requirements of Cd2+ detection with very high sensitivity and specificity, which also demonstrates that genetic negative feedback amplifiers are excellent tools for improving the performance of WCBs.
format	article
author	Guangbao Zhang Shuting Hu Xiaoqiang Jia Xiaoqiang Jia Xiaoqiang Jia
author_facet	Guangbao Zhang Shuting Hu Xiaoqiang Jia Xiaoqiang Jia Xiaoqiang Jia
author_sort	Guangbao Zhang
title	Highly Sensitive Whole-Cell Biosensor for Cadmium Detection Based on a Negative Feedback Circuit
title_short	Highly Sensitive Whole-Cell Biosensor for Cadmium Detection Based on a Negative Feedback Circuit
title_full	Highly Sensitive Whole-Cell Biosensor for Cadmium Detection Based on a Negative Feedback Circuit
title_fullStr	Highly Sensitive Whole-Cell Biosensor for Cadmium Detection Based on a Negative Feedback Circuit
title_full_unstemmed	Highly Sensitive Whole-Cell Biosensor for Cadmium Detection Based on a Negative Feedback Circuit
title_sort	highly sensitive whole-cell biosensor for cadmium detection based on a negative feedback circuit
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/a9785158a9e04c0888ac9772f0c23e81
work_keys_str_mv	AT guangbaozhang highlysensitivewholecellbiosensorforcadmiumdetectionbasedonanegativefeedbackcircuit AT shutinghu highlysensitivewholecellbiosensorforcadmiumdetectionbasedonanegativefeedbackcircuit AT xiaoqiangjia highlysensitivewholecellbiosensorforcadmiumdetectionbasedonanegativefeedbackcircuit AT xiaoqiangjia highlysensitivewholecellbiosensorforcadmiumdetectionbasedonanegativefeedbackcircuit AT xiaoqiangjia highlysensitivewholecellbiosensorforcadmiumdetectionbasedonanegativefeedbackcircuit
_version_	1718373889674838016

Highly Sensitive Whole-Cell Biosensor for Cadmium Detection Based on a Negative Feedback Circuit

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