PEG-coated and Gd-loaded fluorescent silica nanoparticles for targeted prostate cancer magnetic resonance imaging and fluorescence imaging

Wei Jiang,*,1 Huiying Fang,*,2 Fengqiu Liu,3 Xue Zhou,1 Hongyun Zhao,4 Xiaojing He,1 Dajing Guo11Department of Radiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People’s Republic of China; 2Department of Breast Diseases, Chongqin...

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Autores principales:	Jiang W, Fang H, Liu F, Zhou X, Zhao H, He X, Guo D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	silica nanoparticles prostate cancer magnetic resonance imaging fluorescence imaging target Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a97b32aed5024934a2132ece8daba344
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spelling	oai:doaj.org-article:a97b32aed5024934a2132ece8daba3442021-12-02T03:02:46ZPEG-coated and Gd-loaded fluorescent silica nanoparticles for targeted prostate cancer magnetic resonance imaging and fluorescence imaging1178-2013https://doaj.org/article/a97b32aed5024934a2132ece8daba3442019-07-01T00:00:00Zhttps://www.dovepress.com/peg-coated-and-gd-loaded-fluorescent-silica-nanoparticles-for-targeted-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wei Jiang,,1 Huiying Fang,,2 Fengqiu Liu,3 Xue Zhou,1 Hongyun Zhao,4 Xiaojing He,1 Dajing Guo11Department of Radiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People’s Republic of China; 2Department of Breast Diseases, Chongqing University Cancer Hospital, Chongqing Cancer Institute and Chongqing Cancer Hospital, Chongqing 400030, People’s Republic of China; 3Institute of Ultrasound Imaging, Department of Ultrasound, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People’s Republic of China; 4Department of Gastroenterology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People’s Republic of China*These authors contributed equally to this workBackground: Multimodal imaging probes have become a powerful tool for improving detection sensitivity and accuracy, which are important in disease diagnosis and treatment.Methods: In this study, novel bifunctional magnetic resonance imaging (MRI)/fluorescence probes were prepared by loading gadodiamide into fluorescent silica nanoparticles (NPs) (Gd@Cy5.5@SiO2-PEG-Ab NPs) for targeting of prostate cancer (PCa). The physicochemical characteristics, biosafety and PCa cell targeting ability of the Gd@Cy5.5@SiO2-PEG-Ab NPs were studied in vitro and in vivo.Results: The Gd@Cy5.5@SiO2-PEG-Ab NPs had a spherical morphology with a relatively uniform size distribution and demonstrated high efficiency for Gd loading. In vitro and in vivo cell-targeting experiments demonstrated a high potential for the synthesized NPs to target prostate-specific membrane antigen (PSMA) receptor-positive PCa cells, enabling MRI and fluorescence imaging. In vitro cytotoxicity assays and in vivo hematological and pathological assays showed that the prepared NPs exhibited good biological safety.Conclusion: Our study demonstrates that the synthesized Gd@Cy5.5@SiO2-PEG-Ab NPs have great potential as MRI/fluorescence contrast agents for specific identification of PSMA receptor-positive PCa cells.Keywords: silica nanoparticles, prostate cancer, magnetic resonance imaging, fluorescence imaging, targetingJiang WFang HLiu FZhou XZhao HHe XGuo DDove Medical Pressarticlesilica nanoparticlesprostate cancermagnetic resonance imagingfluorescence imagingtargetMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 5611-5622 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	silica nanoparticles prostate cancer magnetic resonance imaging fluorescence imaging target Medicine (General) R5-920
spellingShingle	silica nanoparticles prostate cancer magnetic resonance imaging fluorescence imaging target Medicine (General) R5-920 Jiang W Fang H Liu F Zhou X Zhao H He X Guo D PEG-coated and Gd-loaded fluorescent silica nanoparticles for targeted prostate cancer magnetic resonance imaging and fluorescence imaging
description	Wei Jiang,,1 Huiying Fang,,2 Fengqiu Liu,3 Xue Zhou,1 Hongyun Zhao,4 Xiaojing He,1 Dajing Guo11Department of Radiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People’s Republic of China; 2Department of Breast Diseases, Chongqing University Cancer Hospital, Chongqing Cancer Institute and Chongqing Cancer Hospital, Chongqing 400030, People’s Republic of China; 3Institute of Ultrasound Imaging, Department of Ultrasound, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People’s Republic of China; 4Department of Gastroenterology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People’s Republic of China*These authors contributed equally to this workBackground: Multimodal imaging probes have become a powerful tool for improving detection sensitivity and accuracy, which are important in disease diagnosis and treatment.Methods: In this study, novel bifunctional magnetic resonance imaging (MRI)/fluorescence probes were prepared by loading gadodiamide into fluorescent silica nanoparticles (NPs) (Gd@Cy5.5@SiO2-PEG-Ab NPs) for targeting of prostate cancer (PCa). The physicochemical characteristics, biosafety and PCa cell targeting ability of the Gd@Cy5.5@SiO2-PEG-Ab NPs were studied in vitro and in vivo.Results: The Gd@Cy5.5@SiO2-PEG-Ab NPs had a spherical morphology with a relatively uniform size distribution and demonstrated high efficiency for Gd loading. In vitro and in vivo cell-targeting experiments demonstrated a high potential for the synthesized NPs to target prostate-specific membrane antigen (PSMA) receptor-positive PCa cells, enabling MRI and fluorescence imaging. In vitro cytotoxicity assays and in vivo hematological and pathological assays showed that the prepared NPs exhibited good biological safety.Conclusion: Our study demonstrates that the synthesized Gd@Cy5.5@SiO2-PEG-Ab NPs have great potential as MRI/fluorescence contrast agents for specific identification of PSMA receptor-positive PCa cells.Keywords: silica nanoparticles, prostate cancer, magnetic resonance imaging, fluorescence imaging, targeting
format	article
author	Jiang W Fang H Liu F Zhou X Zhao H He X Guo D
author_facet	Jiang W Fang H Liu F Zhou X Zhao H He X Guo D
author_sort	Jiang W
title	PEG-coated and Gd-loaded fluorescent silica nanoparticles for targeted prostate cancer magnetic resonance imaging and fluorescence imaging
title_short	PEG-coated and Gd-loaded fluorescent silica nanoparticles for targeted prostate cancer magnetic resonance imaging and fluorescence imaging
title_full	PEG-coated and Gd-loaded fluorescent silica nanoparticles for targeted prostate cancer magnetic resonance imaging and fluorescence imaging
title_fullStr	PEG-coated and Gd-loaded fluorescent silica nanoparticles for targeted prostate cancer magnetic resonance imaging and fluorescence imaging
title_full_unstemmed	PEG-coated and Gd-loaded fluorescent silica nanoparticles for targeted prostate cancer magnetic resonance imaging and fluorescence imaging
title_sort	peg-coated and gd-loaded fluorescent silica nanoparticles for targeted prostate cancer magnetic resonance imaging and fluorescence imaging
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/a97b32aed5024934a2132ece8daba344
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PEG-coated and Gd-loaded fluorescent silica nanoparticles for targeted prostate cancer magnetic resonance imaging and fluorescence imaging

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