Small RNA mediated repression of subtilisin production in Bacillus licheniformis

Abstract The species Bacillus licheniformis includes important strains that are used in industrial production processes. Currently the physiological model used to adapt these processes is based on the closely related model organism B. subtilis. In this study we found that both organisms reveal signi...

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Autores principales:	Robert Hertel, Sandra Meyerjürgens, Birgit Voigt, Heiko Liesegang, Sonja Volland
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a97c24e7d2264ecfaffff766b5a3102c
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spelling	oai:doaj.org-article:a97c24e7d2264ecfaffff766b5a3102c2021-12-02T15:05:47ZSmall RNA mediated repression of subtilisin production in Bacillus licheniformis10.1038/s41598-017-05628-y2045-2322https://doaj.org/article/a97c24e7d2264ecfaffff766b5a3102c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05628-yhttps://doaj.org/toc/2045-2322Abstract The species Bacillus licheniformis includes important strains that are used in industrial production processes. Currently the physiological model used to adapt these processes is based on the closely related model organism B. subtilis. In this study we found that both organisms reveal significant differences in the regulation of subtilisin, their main natural protease and a product of industrial fermentation processes. We identified and characterized a novel antisense sRNA AprAs, which represents an RNA based repressor of apr, the gene encoding for the industrial relevant subtilisin protease. Reduction of the AprAs level leads to an enhanced proteolytic activity and an increase of Apr protein expression in the mutant strain. A vector based complementation of the AprAs deficient mutant confirmed this effect and demonstrated the necessity of cis transcription for full efficiency. A comparative analysis of the corresponding genome loci from B. licheniformis and B. subtilis revealed the absence of an aprAs promoter in B. subtilis and indicates that AprAs is a B. licheniformis species specific phenomenon. The discovery of AprAs is of great biotechnological interest since subtilisin Carlsberg is one of the main products of industrial fermentation by B. licheniformis.Robert HertelSandra MeyerjürgensBirgit VoigtHeiko LiesegangSonja VollandNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Robert Hertel Sandra Meyerjürgens Birgit Voigt Heiko Liesegang Sonja Volland Small RNA mediated repression of subtilisin production in Bacillus licheniformis
description	Abstract The species Bacillus licheniformis includes important strains that are used in industrial production processes. Currently the physiological model used to adapt these processes is based on the closely related model organism B. subtilis. In this study we found that both organisms reveal significant differences in the regulation of subtilisin, their main natural protease and a product of industrial fermentation processes. We identified and characterized a novel antisense sRNA AprAs, which represents an RNA based repressor of apr, the gene encoding for the industrial relevant subtilisin protease. Reduction of the AprAs level leads to an enhanced proteolytic activity and an increase of Apr protein expression in the mutant strain. A vector based complementation of the AprAs deficient mutant confirmed this effect and demonstrated the necessity of cis transcription for full efficiency. A comparative analysis of the corresponding genome loci from B. licheniformis and B. subtilis revealed the absence of an aprAs promoter in B. subtilis and indicates that AprAs is a B. licheniformis species specific phenomenon. The discovery of AprAs is of great biotechnological interest since subtilisin Carlsberg is one of the main products of industrial fermentation by B. licheniformis.
format	article
author	Robert Hertel Sandra Meyerjürgens Birgit Voigt Heiko Liesegang Sonja Volland
author_facet	Robert Hertel Sandra Meyerjürgens Birgit Voigt Heiko Liesegang Sonja Volland
author_sort	Robert Hertel
title	Small RNA mediated repression of subtilisin production in Bacillus licheniformis
title_short	Small RNA mediated repression of subtilisin production in Bacillus licheniformis
title_full	Small RNA mediated repression of subtilisin production in Bacillus licheniformis
title_fullStr	Small RNA mediated repression of subtilisin production in Bacillus licheniformis
title_full_unstemmed	Small RNA mediated repression of subtilisin production in Bacillus licheniformis
title_sort	small rna mediated repression of subtilisin production in bacillus licheniformis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a97c24e7d2264ecfaffff766b5a3102c
work_keys_str_mv	AT roberthertel smallrnamediatedrepressionofsubtilisinproductioninbacilluslicheniformis AT sandrameyerjurgens smallrnamediatedrepressionofsubtilisinproductioninbacilluslicheniformis AT birgitvoigt smallrnamediatedrepressionofsubtilisinproductioninbacilluslicheniformis AT heikoliesegang smallrnamediatedrepressionofsubtilisinproductioninbacilluslicheniformis AT sonjavolland smallrnamediatedrepressionofsubtilisinproductioninbacilluslicheniformis
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Small RNA mediated repression of subtilisin production in Bacillus licheniformis

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