Anaerobic methanotrophic communities thrive in deep submarine permafrost

Abstract Thawing submarine permafrost is a source of methane to the subsurface biosphere. Methane oxidation in submarine permafrost sediments has been proposed, but the responsible microorganisms remain uncharacterized. We analyzed archaeal communities and identified distinct anaerobic methanotrophi...

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Autores principales:	Matthias Winkel, Julia Mitzscherling, Pier P. Overduin, Fabian Horn, Maria Winterfeld, Ruud Rijkers, Mikhail N. Grigoriev, Christian Knoblauch, Kai Mangelsdorf, Dirk Wagner, Susanne Liebner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a97ecf82a7f745799feaf8a831c7f5af
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spelling	oai:doaj.org-article:a97ecf82a7f745799feaf8a831c7f5af2021-12-02T15:09:03ZAnaerobic methanotrophic communities thrive in deep submarine permafrost10.1038/s41598-018-19505-92045-2322https://doaj.org/article/a97ecf82a7f745799feaf8a831c7f5af2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19505-9https://doaj.org/toc/2045-2322Abstract Thawing submarine permafrost is a source of methane to the subsurface biosphere. Methane oxidation in submarine permafrost sediments has been proposed, but the responsible microorganisms remain uncharacterized. We analyzed archaeal communities and identified distinct anaerobic methanotrophic assemblages of marine and terrestrial origin (ANME-2a/b, ANME-2d) both in frozen and completely thawed submarine permafrost sediments. Besides archaea potentially involved in anaerobic oxidation of methane (AOM) we found a large diversity of archaea mainly belonging to Bathyarchaeota, Thaumarchaeota, and Euryarchaeota. Methane concentrations and δ13C-methane signatures distinguish horizons of potential AOM coupled either to sulfate reduction in a sulfate-methane transition zone (SMTZ) or to the reduction of other electron acceptors, such as iron, manganese or nitrate. Analysis of functional marker genes (mcrA) and fluorescence in situ hybridization (FISH) corroborate potential activity of AOM communities in submarine permafrost sediments at low temperatures. Modeled potential AOM consumes 72–100% of submarine permafrost methane and up to 1.2 Tg of carbon per year for the total expected area of submarine permafrost. This is comparable with AOM habitats such as cold seeps. We thus propose that AOM is active where submarine permafrost thaws, which should be included in global methane budgets.Matthias WinkelJulia MitzscherlingPier P. OverduinFabian HornMaria WinterfeldRuud RijkersMikhail N. GrigorievChristian KnoblauchKai MangelsdorfDirk WagnerSusanne LiebnerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Matthias Winkel Julia Mitzscherling Pier P. Overduin Fabian Horn Maria Winterfeld Ruud Rijkers Mikhail N. Grigoriev Christian Knoblauch Kai Mangelsdorf Dirk Wagner Susanne Liebner Anaerobic methanotrophic communities thrive in deep submarine permafrost
description	Abstract Thawing submarine permafrost is a source of methane to the subsurface biosphere. Methane oxidation in submarine permafrost sediments has been proposed, but the responsible microorganisms remain uncharacterized. We analyzed archaeal communities and identified distinct anaerobic methanotrophic assemblages of marine and terrestrial origin (ANME-2a/b, ANME-2d) both in frozen and completely thawed submarine permafrost sediments. Besides archaea potentially involved in anaerobic oxidation of methane (AOM) we found a large diversity of archaea mainly belonging to Bathyarchaeota, Thaumarchaeota, and Euryarchaeota. Methane concentrations and δ13C-methane signatures distinguish horizons of potential AOM coupled either to sulfate reduction in a sulfate-methane transition zone (SMTZ) or to the reduction of other electron acceptors, such as iron, manganese or nitrate. Analysis of functional marker genes (mcrA) and fluorescence in situ hybridization (FISH) corroborate potential activity of AOM communities in submarine permafrost sediments at low temperatures. Modeled potential AOM consumes 72–100% of submarine permafrost methane and up to 1.2 Tg of carbon per year for the total expected area of submarine permafrost. This is comparable with AOM habitats such as cold seeps. We thus propose that AOM is active where submarine permafrost thaws, which should be included in global methane budgets.
format	article
author	Matthias Winkel Julia Mitzscherling Pier P. Overduin Fabian Horn Maria Winterfeld Ruud Rijkers Mikhail N. Grigoriev Christian Knoblauch Kai Mangelsdorf Dirk Wagner Susanne Liebner
author_facet	Matthias Winkel Julia Mitzscherling Pier P. Overduin Fabian Horn Maria Winterfeld Ruud Rijkers Mikhail N. Grigoriev Christian Knoblauch Kai Mangelsdorf Dirk Wagner Susanne Liebner
author_sort	Matthias Winkel
title	Anaerobic methanotrophic communities thrive in deep submarine permafrost
title_short	Anaerobic methanotrophic communities thrive in deep submarine permafrost
title_full	Anaerobic methanotrophic communities thrive in deep submarine permafrost
title_fullStr	Anaerobic methanotrophic communities thrive in deep submarine permafrost
title_full_unstemmed	Anaerobic methanotrophic communities thrive in deep submarine permafrost
title_sort	anaerobic methanotrophic communities thrive in deep submarine permafrost
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a97ecf82a7f745799feaf8a831c7f5af
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Anaerobic methanotrophic communities thrive in deep submarine permafrost

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