RNA-Seq reveals infection-related gene expression changes in Phytophthora capsici.

Phytophthora capsici is a soilborne plant pathogen capable of infecting a wide range of plants, including many solanaceous crops. However, genetic resistance and fungicides often fail to manage P. capsici due to limited knowledge on the molecular biology and basis of P. capsici pathogenicity. To beg...

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Autores principales:	Xiao-Ren Chen, Yu-Ping Xing, Yan-Peng Li, Yun-Hui Tong, Jing-You Xu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a9851c05b92f408a82caee64413a3ed4
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spelling	oai:doaj.org-article:a9851c05b92f408a82caee64413a3ed42021-11-18T08:57:07ZRNA-Seq reveals infection-related gene expression changes in Phytophthora capsici.1932-620310.1371/journal.pone.0074588https://doaj.org/article/a9851c05b92f408a82caee64413a3ed42013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24019970/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Phytophthora capsici is a soilborne plant pathogen capable of infecting a wide range of plants, including many solanaceous crops. However, genetic resistance and fungicides often fail to manage P. capsici due to limited knowledge on the molecular biology and basis of P. capsici pathogenicity. To begin to rectify this situation, Illumina RNA-Seq was used to perform massively parallel sequencing of three cDNA samples derived from P. capsici mycelia (MY), zoospores (ZO) and germinating cysts with germ tubes (GC). Over 11 million reads were generated for each cDNA library analyzed. After read mapping to the gene models of P. capsici reference genome, 13,901, 14,633 and 14,695 putative genes were identified from the reads of the MY, ZO and GC libraries, respectively. Comparative analysis between two of samples showed major differences between the expressed gene content of MY, ZO and GC stages. A large number of genes associated with specific stages and pathogenicity were identified, including 98 predicted effector genes. The transcriptional levels of 19 effector genes during the developmental and host infection stages of P. capsici were validated by RT-PCR. Ectopic expression in Nicotiana benthamiana showed that P. capsici RXLR and Crinkler effectors can suppress host cell death triggered by diverse elicitors including P. capsici elicitin and NLP effectors. This study provides a first look at the transcriptome and effector arsenal of P. capsici during the important pre-infection stages.Xiao-Ren ChenYu-Ping XingYan-Peng LiYun-Hui TongJing-You XuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e74588 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiao-Ren Chen Yu-Ping Xing Yan-Peng Li Yun-Hui Tong Jing-You Xu RNA-Seq reveals infection-related gene expression changes in Phytophthora capsici.
description	Phytophthora capsici is a soilborne plant pathogen capable of infecting a wide range of plants, including many solanaceous crops. However, genetic resistance and fungicides often fail to manage P. capsici due to limited knowledge on the molecular biology and basis of P. capsici pathogenicity. To begin to rectify this situation, Illumina RNA-Seq was used to perform massively parallel sequencing of three cDNA samples derived from P. capsici mycelia (MY), zoospores (ZO) and germinating cysts with germ tubes (GC). Over 11 million reads were generated for each cDNA library analyzed. After read mapping to the gene models of P. capsici reference genome, 13,901, 14,633 and 14,695 putative genes were identified from the reads of the MY, ZO and GC libraries, respectively. Comparative analysis between two of samples showed major differences between the expressed gene content of MY, ZO and GC stages. A large number of genes associated with specific stages and pathogenicity were identified, including 98 predicted effector genes. The transcriptional levels of 19 effector genes during the developmental and host infection stages of P. capsici were validated by RT-PCR. Ectopic expression in Nicotiana benthamiana showed that P. capsici RXLR and Crinkler effectors can suppress host cell death triggered by diverse elicitors including P. capsici elicitin and NLP effectors. This study provides a first look at the transcriptome and effector arsenal of P. capsici during the important pre-infection stages.
format	article
author	Xiao-Ren Chen Yu-Ping Xing Yan-Peng Li Yun-Hui Tong Jing-You Xu
author_facet	Xiao-Ren Chen Yu-Ping Xing Yan-Peng Li Yun-Hui Tong Jing-You Xu
author_sort	Xiao-Ren Chen
title	RNA-Seq reveals infection-related gene expression changes in Phytophthora capsici.
title_short	RNA-Seq reveals infection-related gene expression changes in Phytophthora capsici.
title_full	RNA-Seq reveals infection-related gene expression changes in Phytophthora capsici.
title_fullStr	RNA-Seq reveals infection-related gene expression changes in Phytophthora capsici.
title_full_unstemmed	RNA-Seq reveals infection-related gene expression changes in Phytophthora capsici.
title_sort	rna-seq reveals infection-related gene expression changes in phytophthora capsici.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a9851c05b92f408a82caee64413a3ed4
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RNA-Seq reveals infection-related gene expression changes in Phytophthora capsici.

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