circTLK1 facilitates the proliferation and metastasis of renal cell carcinoma by regulating miR-495-3p/CBL axis

Renal cell carcinoma (RCC) is a common urological malignancy. Circular RNAs (circRNAs) have been confirmed to play an important regulatory role in various cancers. This study aimed to investigate the role and potential mechanism of circTLK1 (hsa_circ_0004442) in RCC. The levels of circTLK1, Cbl prot...

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Autores principales:	Lei Xiangli, Yang Meiling, Xiao Zhifang, Zhang Heng, Tan Shuai
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	circtlk1 mir-495-3p cbl renal cell carcinoma Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a98804189b9f4ad393484e4e697f019e
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spelling	oai:doaj.org-article:a98804189b9f4ad393484e4e697f019e2021-12-05T14:10:41ZcircTLK1 facilitates the proliferation and metastasis of renal cell carcinoma by regulating miR-495-3p/CBL axis2391-541210.1515/biol-2021-0041https://doaj.org/article/a98804189b9f4ad393484e4e697f019e2021-04-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0041https://doaj.org/toc/2391-5412Renal cell carcinoma (RCC) is a common urological malignancy. Circular RNAs (circRNAs) have been confirmed to play an important regulatory role in various cancers. This study aimed to investigate the role and potential mechanism of circTLK1 (hsa_circ_0004442) in RCC. The levels of circTLK1, Cbl proto-oncogene (CBL), and microRNA-495-3p (miR-495-3p) were detected by quantitative reverse transcription polymerase chain reaction or western blot. Cell proliferation, cycle arrest and apoptosis, migration, and invasion were assessed by colony formation, flow cytometry, scratch, and transwell assays. The levels of E-cadherin and Vimentin were measured by western blot. The targeting relationship between miR-495-3p and miR-495-3p or CBL was verified by dual-luciferase reporter assay. Tumor growth in vivo was evaluated by xenograft assay. The results found that circTLK1 and CBL were up-regulated in RCC tissues and cells. Silencing of circTLK1 or CBL inhibited proliferation and metastasis and accelerated apoptosis in RCC cells. In addition, circTLK1 directly bound to miR-495-3p, and CBL was the target of miR-495-3p. circTLK1 sponged miR-495-3p to increase CBL expression. Moreover, knockdown of circTLK1 suppressed tumor growth in vivo. In conclusion, down-regulation of circTLK1 restrained proliferation and metastasis and promoted apoptosis in RCC cells by modulating miR-495-3p/CBL axis.Lei XiangliYang MeilingXiao ZhifangZhang HengTan ShuaiDe Gruyterarticlecirctlk1mir-495-3pcblrenal cell carcinomaBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 362-374 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circtlk1 mir-495-3p cbl renal cell carcinoma Biology (General) QH301-705.5
spellingShingle	circtlk1 mir-495-3p cbl renal cell carcinoma Biology (General) QH301-705.5 Lei Xiangli Yang Meiling Xiao Zhifang Zhang Heng Tan Shuai circTLK1 facilitates the proliferation and metastasis of renal cell carcinoma by regulating miR-495-3p/CBL axis
description	Renal cell carcinoma (RCC) is a common urological malignancy. Circular RNAs (circRNAs) have been confirmed to play an important regulatory role in various cancers. This study aimed to investigate the role and potential mechanism of circTLK1 (hsa_circ_0004442) in RCC. The levels of circTLK1, Cbl proto-oncogene (CBL), and microRNA-495-3p (miR-495-3p) were detected by quantitative reverse transcription polymerase chain reaction or western blot. Cell proliferation, cycle arrest and apoptosis, migration, and invasion were assessed by colony formation, flow cytometry, scratch, and transwell assays. The levels of E-cadherin and Vimentin were measured by western blot. The targeting relationship between miR-495-3p and miR-495-3p or CBL was verified by dual-luciferase reporter assay. Tumor growth in vivo was evaluated by xenograft assay. The results found that circTLK1 and CBL were up-regulated in RCC tissues and cells. Silencing of circTLK1 or CBL inhibited proliferation and metastasis and accelerated apoptosis in RCC cells. In addition, circTLK1 directly bound to miR-495-3p, and CBL was the target of miR-495-3p. circTLK1 sponged miR-495-3p to increase CBL expression. Moreover, knockdown of circTLK1 suppressed tumor growth in vivo. In conclusion, down-regulation of circTLK1 restrained proliferation and metastasis and promoted apoptosis in RCC cells by modulating miR-495-3p/CBL axis.
format	article
author	Lei Xiangli Yang Meiling Xiao Zhifang Zhang Heng Tan Shuai
author_facet	Lei Xiangli Yang Meiling Xiao Zhifang Zhang Heng Tan Shuai
author_sort	Lei Xiangli
title	circTLK1 facilitates the proliferation and metastasis of renal cell carcinoma by regulating miR-495-3p/CBL axis
title_short	circTLK1 facilitates the proliferation and metastasis of renal cell carcinoma by regulating miR-495-3p/CBL axis
title_full	circTLK1 facilitates the proliferation and metastasis of renal cell carcinoma by regulating miR-495-3p/CBL axis
title_fullStr	circTLK1 facilitates the proliferation and metastasis of renal cell carcinoma by regulating miR-495-3p/CBL axis
title_full_unstemmed	circTLK1 facilitates the proliferation and metastasis of renal cell carcinoma by regulating miR-495-3p/CBL axis
title_sort	circtlk1 facilitates the proliferation and metastasis of renal cell carcinoma by regulating mir-495-3p/cbl axis
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/a98804189b9f4ad393484e4e697f019e
work_keys_str_mv	AT leixiangli circtlk1facilitatestheproliferationandmetastasisofrenalcellcarcinomabyregulatingmir4953pcblaxis AT yangmeiling circtlk1facilitatestheproliferationandmetastasisofrenalcellcarcinomabyregulatingmir4953pcblaxis AT xiaozhifang circtlk1facilitatestheproliferationandmetastasisofrenalcellcarcinomabyregulatingmir4953pcblaxis AT zhangheng circtlk1facilitatestheproliferationandmetastasisofrenalcellcarcinomabyregulatingmir4953pcblaxis AT tanshuai circtlk1facilitatestheproliferationandmetastasisofrenalcellcarcinomabyregulatingmir4953pcblaxis
_version_	1718371858849464320

circTLK1 facilitates the proliferation and metastasis of renal cell carcinoma by regulating miR-495-3p/CBL axis

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